Restriction map for
pF1KB3024
(Circular) (Six-base) MAPSORT of: pF1KB3024.seq Check: 3400 from: 1 to: 7169
pF1KB3024 7169 bp
With 182 enzymes: SgfI *
December 26, 2011 13:17 ..
AatII G_ACGT'C
Cuts at: 2356 2356
Size: 7169
AccI GT'mk_AC
Cuts at: 1264 2600 3251 4207 1264
Size: 1336 651 956 4226
Fragments arranged by size:
4226 1336 956 651
Acc65I G'GTAC_C
Cuts at: 3471 4185 3471
Size: 714 6455
AclI AA'CG_TT
Cuts at: 7009 7009
Size: 7169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3617 4020 4994 5426 6173 6441 3617
Size: 403 974 432 747 268 4345
Fragments arranged by size:
4345 974 747 432 403 268
AfeI AGC'GCT
Cuts at: 3352 3352
Size: 7169
AflIII A'CryG_T
Cuts at: 1229 4521 5625 6602 1229
Size: 3292 1104 977 1796
Fragments arranged by size:
3292 1796 1104 977
AgeI A'CCGG_T
Cuts at: 5557 5557
Size: 7169
AhdI GACnn_n'nnGTC
Cuts at: 1292 1292
Size: 7169
AleI CACnn'nnGTG
Cuts at: 469 1386 2297 3104 469
Size: 917 911 807 4534
Fragments arranged by size:
4534 917 911 807
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1136 1136
Size: 7169
AlwNI CAG_nnn'CTG
Cuts at: 693 1041 1680 2075 2862 3502 4474 6041 693
Size: 348 639 395 787 640 972 1567 1821
Fragments arranged by size:
1821 1567 972 787 640 639 395 348
ApaI G_GGCC'C
Cuts at: 292 1138 1973 3385 3865 292
Size: 846 835 1412 480 3596
Fragments arranged by size:
3596 1412 846 835 480
ApaLI G'TGCA_C
Cuts at: 474 1828 5939 474
Size: 1354 4111 1704
Fragments arranged by size:
4111 1704 1354
ApoI r'AATT_y
Cuts at: 1700 2639 3322 4173 6522 6778 1700
Size: 939 683 851 2349 256 2091
Fragments arranged by size:
2349 2091 939 851 683 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7169
AvaI C'yCGr_G
Cuts at: 260 514 965 1252 1415 1946 3182 3507 3854
Size: 254 451 287 163 531 1236 325 347
Cuts at: 3854 3979 4189 260
Size: 125 210 3240
Fragments arranged by size:
3240 1236 531 451 347 325 287 254
210 163 125
BamHI G'GATC_C
Cuts at: 1324 2414 2537 2615 3173 4194 1324
Size: 1090 123 78 558 1021 4299
Fragments arranged by size:
4299 1090 1021 558 123 78
BanI G'GyrC_C
Cuts at: 709 1273 1714 1836 3048 3471 3536 3624 3889
Size: 564 441 122 1212 423 65 88 265
Cuts at: 3889 3964 4185 4599 4847 4882 709
Size: 75 221 414 248 35 2996
Fragments arranged by size:
2996 1212 564 441 423 414 265 248
221 122 88 75 65 35
BanII G_rGCy'C
Cuts at: 258 292 431 1003 1138 1973 2048 2734 2854
Size: 34 139 572 135 835 75 686 120
Cuts at: 2854 3385 3593 3865 4095 4183 5213 258
Size: 531 208 272 230 88 1030 2214
Fragments arranged by size:
2214 1030 835 686 572 531 272 230
208 139 135 120 88 75 34
BbeI G_GCGC'C
Cuts at: 1277 1718 4603 4851 1277
Size: 441 2885 248 3595
Fragments arranged by size:
3595 2885 441 248
BbsI GAAGACnn'nnnn_
Cuts at: 1662 1662
Size: 7169
BbvCI CC'TCA_GC
Cuts at: 1663 2557 3458 4013 1663
Size: 894 901 555 4819
Fragments arranged by size:
4819 901 894 555
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1937 1971 90
Size: 34 1813 34 5288
Fragments arranged by size:
5288 1813 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5067 5828 7169 5067
Size: 761 1341 5067
Fragments arranged by size:
5067 1341 761
BclI T'GATC_A
Cuts at: 3257 4689 3257
Size: 1432 5737
BglI GCCn_nnn'nGGC
Cuts at: 262 2756 262
Size: 2494 4675
BglII A'GATC_T
Cuts at: 839 1505 4684 6721 839
Size: 666 3179 2037 1287
Fragments arranged by size:
3179 2037 1287 666
BlpI GC'TnA_GC
Cuts at: 4281 4281
Size: 7169
Bme1580I G_kGCm'C
Cuts at: 292 478 1074 1138 1416 1832 1841 1955 1973
Size: 186 596 64 278 416 9 114 18
Cuts at: 1973 2163 3053 3385 3865 4794 4887 5943 292
Size: 190 890 332 480 929 93 1056 1518
Fragments arranged by size:
1518 1056 929 890 596 480 416 332
278 190 186 114 93 64 18 9
BmgBI CAC'GTC
Cuts at: 1320 4155 1320
Size: 2835 4334
BmrI ACTGGGnnnn_n'
Cuts at: 1789 1971 4074 4381 4462 4797 6941 1789
Size: 182 2103 307 81 335 2144 2017
Fragments arranged by size:
2144 2103 2017 335 307 182 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5536 5536
Size: 7169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1558 2926 3091 3445 3607 1558
Size: 1368 165 354 162 5120
Fragments arranged by size:
5120 1368 354 165 162
Bpu10I CC'TnA_GC
Cuts at: 1663 2557 3458 4013 1663
Size: 894 901 555 4819
Fragments arranged by size:
4819 901 894 555
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 4345 5224 5716 6014 207
Size: 4138 879 492 298 1362
Fragments arranged by size:
4138 1362 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 511 752 3620 6873 511
Size: 241 2868 3253 807
Fragments arranged by size:
3253 2868 807 241
BsaAI yAC'GTr
Cuts at: 648 2715 4522 648
Size: 2067 1807 3295
Fragments arranged by size:
3295 2067 1807
BsaBI GATnn'nnATC
Cuts at: 6 2419 2536 4683 6720 6
Size: 2413 117 2147 2037 455
Fragments arranged by size:
2413 2147 2037 455 117
BsaHI Gr'CG_yC
Cuts at: 1274 1715 2353 2844 4600 4848 5550 7046 1274
Size: 441 638 491 1756 248 702 1496 1397
Fragments arranged by size:
1756 1496 1397 702 638 491 441 248
BsaWI w'CCGG_w
Cuts at: 238 2133 2444 4358 4879 5557 5831 5978 6389
Size: 1895 311 1914 521 678 274 147 411
Cuts at: 6389 238
Size: 1018
Fragments arranged by size:
1914 1895 1018 678 521 411 311 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 374 404 1540 1570 3108 3138 3301 3331 3549
Size: 30 1136 30 1538 30 163 30 218
Cuts at: 3549 3579 374
Size: 30 3964
Fragments arranged by size:
3964 1538 1136 218 163 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 187 190 421 424 2269 3475 3616 4016
Size: 9 3 231 3 1845 1206 141 400
Cuts at: 4016 178
Size: 3331
Fragments arranged by size:
3331 1845 1206 400 231 141 9 3
3
BseYI C'CCAG_C
Cuts at: 128 351 422 713 875 1010 1203 1551 1740
Size: 223 71 291 162 135 193 348 189
Cuts at: 1740 1755 1958 2200 3177 3224 3347 3384 3476
Size: 15 203 242 977 47 123 37 92
Cuts at: 3476 3514 3715 3803 3839 4041 4099 4594 5352
Size: 38 201 88 36 202 58 495 758
Cuts at: 5352 5929 6425 128
Size: 577 496 872
Fragments arranged by size:
977 872 758 577 496 495 348 291
242 223 203 202 201 193 189 162
135 123 92 88 71 58 47 38
37 36 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 586 658 1113 1180 1314 1333 1419 1690 2271
Size: 72 455 67 134 19 86 271 581
Cuts at: 2271 2800 3222 586
Size: 529 422 4533
Fragments arranged by size:
4533 581 529 455 422 271 134 86
72 67 19
BsiEI CG_ry'CG
Cuts at: 104 4711 4757 5965 104
Size: 4607 46 1208 1308
Fragments arranged by size:
4607 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 397 466 478 653 1445 1832 4183 4961 5151
Size: 69 12 175 792 387 2351 778 190
Cuts at: 5151 5943 397
Size: 792 1623
Fragments arranged by size:
2351 1623 792 792 778 387 190 175
69 12
BsmI GAATG_Cn'
Cuts at: 2032 2070 2378 2032
Size: 38 308 6823
Fragments arranged by size:
6823 308 38
BsmBI CGTCTCn'nnnn_
Cuts at: 2542 2542
Size: 7169
Bsp1286I G_dGCh'C
Cuts at: 258 292 397 431 466 478 653 1003 1074
Size: 34 105 34 35 12 175 350 71
Cuts at: 1074 1138 1416 1445 1832 1841 1955 1973 2048
Size: 64 278 29 387 9 114 18 75
Cuts at: 2048 2163 2734 2854 3053 3385 3593 3865 4095
Size: 115 571 120 199 332 208 272 230
Cuts at: 4095 4183 4794 4887 4961 5151 5213 5943 258
Size: 88 611 93 74 190 62 730 1484
Fragments arranged by size:
1484 730 611 571 387 350 332 278
272 230 208 199 190 175 120 115
114 105 93 88 75 74 71 64
62 35 34 34 29 18 12 9
BspEI T'CCGG_A
Cuts at: 2444 6389 2444
Size: 3945 3224
BspHI T'CATG_A
Cuts at: 6345 6345
Size: 7169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 599 666 1688 2870 3206 3802 3891 4219 4433
Size: 67 1022 1182 336 596 89 328 214
Cuts at: 4433 4735 5116 599
Size: 302 381 2652
Fragments arranged by size:
2652 1182 1022 596 381 336 328 302
214 89 67
BsrBI CCG'CTC
Cuts at: 50 962 1572 2941 3216 3416 4086 5461 5515
Size: 912 610 1369 275 200 670 1375 54
Cuts at: 5515 6999 7164 50
Size: 1484 165 55
Fragments arranged by size:
1484 1375 1369 912 670 610 275 200
165 55 54
BsrDI GCAATG_nn'
Cuts at: 5081 5081
Size: 7169
BsrFI r'CCGG_y
Cuts at: 478 1060 1123 1259 1892 3086 5167 5348 5557
Size: 582 63 136 633 1194 2081 181 209
Cuts at: 5557 478
Size: 2090
Fragments arranged by size:
2090 2081 1194 633 582 209 181 136
63
BssHII G'CGCG_C
Cuts at: 135 271 305 135
Size: 136 34 6999
Fragments arranged by size:
6999 136 34
BssSI C'ACGA_G
Cuts at: 5440 5798 5440
Size: 358 6811
BstAPI GCAn_nnn'nTGC
Cuts at: 693 1836 2079 4546 693
Size: 1143 243 2467 3316
Fragments arranged by size:
3316 2467 1143 243
BstBI TT'CG_AA
Cuts at: 825 5530 825
Size: 4705 2464
BstEII G'GTnAC_C
Cuts at: 1212 1621 3661 1212
Size: 409 2040 4720
Fragments arranged by size:
4720 2040 409
BstXI CCAn_nnnn'nTGG
Cuts at: 114 405 3037 3396 114
Size: 291 2632 359 3887
Fragments arranged by size:
3887 2632 359 291
BstYI r'GATC_y
Cuts at: 1 839 1324 1505 2414 2441 2537 2578 2615
Size: 838 485 181 909 27 96 41 37
Cuts at: 2615 3173 3188 4194 4661 4684 5019 5265 6277
Size: 558 15 1006 467 23 335 246 1012
Cuts at: 6277 6363 6375 6721 1
Size: 86 12 346 449
Fragments arranged by size:
1012 1006 909 838 558 485 467 449
346 335 246 181 96 86 41 37
27 23 15 12
BstZ17I GTA'TAC
Cuts at: 1265 1265
Size: 7169
BtgI C'CryG_G
Cuts at: 283 372 398 2774 2838 3968 283
Size: 89 26 2376 64 1130 3484
Fragments arranged by size:
3484 2376 1130 89 64 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 639 2527 5119 5300 5463 639
Size: 1888 2592 181 163 2345
Fragments arranged by size:
2592 2345 1888 181 163
BtsI GCAGTG_nn'
Cuts at: 395 682 1087 1120 1197 1562 1791 1883 1953
Size: 287 405 33 77 365 229 92 70
Cuts at: 1953 3418 4412 395
Size: 1465 994 3152
Fragments arranged by size:
3152 1465 994 405 365 287 229 92
77 70 33
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1161 1196 1161
Size: 35 7134
DraI TTT'AAA
Cuts at: 4168 4168
Size: 7169
DraIII CAC_nnn'GTG
Cuts at: 1836 1979 1836
Size: 143 7026
DrdI GACnn_nn'nnGTC
Cuts at: 1666 4875 5733 1666
Size: 3209 858 3102
Fragments arranged by size:
3209 3102 858
EaeI y'GGCC_r
Cuts at: 740 1642 1894 2648 2936 3295 3335 4754 4928
Size: 902 252 754 288 359 40 1419 174
Cuts at: 4928 5319 5346 6691 740
Size: 391 27 1345 1218
Fragments arranged by size:
1419 1345 1218 902 754 391 359 288
252 174 40 27
EagI C'GGCC_G
Cuts at: 4754 4754
Size: 7169
EarI CTCTTCn'nnn_
Cuts at: 1604 3593 5192 5402 1604
Size: 1989 1599 210 3371
Fragments arranged by size:
3371 1989 1599 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 526 3073 5377 5687 5833 6979 526
Size: 2547 2304 310 146 1146 716
Fragments arranged by size:
2547 2304 1146 716 310 146
EcoICRI GAG'CTC
Cuts at: 4181 4181
Size: 7169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1558 2926 3091 3445 3607 3617 4020 4994 5426
Size: 1368 165 354 162 10 403 974 432
Cuts at: 5426 6173 6441 1558
Size: 747 268 2286
Fragments arranged by size:
2286 1368 974 747 432 403 354 268
165 162 10
EcoNI CCTnn'n_nnAGG
Cuts at: 547 785 547
Size: 238 6931
EcoO109I rG'GnC_Cy
Cuts at: 288 289 678 791 1135 1548 1920 1969 2172
Size: 1 389 113 344 413 372 49 203
Cuts at: 2172 2859 3342 3444 3570 3606 3741 3861 3862
Size: 687 483 102 126 36 135 120 1
Cuts at: 3862 4074 4308 288
Size: 212 234 3149
Fragments arranged by size:
3149 687 483 413 389 372 344 234
212 203 135 126 120 113 102 49
36 1 1
EcoRI G'AATT_C
Cuts at: 1700 3322 4173 1700
Size: 1622 851 4696
Fragments arranged by size:
4696 1622 851
EcoRV GAT'ATC
Cuts at: 627 627
Size: 7169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 930 1524 5188 930
Size: 594 3664 2911
Fragments arranged by size:
3664 2911 594
FspI TGC'GCA
Cuts at: 4950 4950
Size: 7169
HaeII r_GCGC'y
Cuts at: 1277 1718 2588 3070 3354 3515 4603 4851 5873
Size: 441 870 482 284 161 1088 248 1022
Cuts at: 5873 6852 1277
Size: 979 1594
Fragments arranged by size:
1594 1088 1022 979 870 482 441 284
248 161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1278 1309 1310 1341 1540 1572 1781 1813 3301
Size: 31 1 31 199 32 209 32 1488
Cuts at: 3301 3333 5011 5041 5043 5073 1278
Size: 32 1678 30 2 30 3374
Fragments arranged by size:
3374 1678 1488 209 199 32 32 32
31 31 30 30 2 1
HincII GTy'rAC
Cuts at: 1986 4208 1986
Size: 2222 4947
KasI G'GCGC_C
Cuts at: 1273 1714 4599 4847 1273
Size: 441 2885 248 3595
Fragments arranged by size:
3595 2885 441 248
KpnI G_GTAC'C
Cuts at: 3475 4189 3475
Size: 714 6455
MluI A'CGCG_T
Cuts at: 6602 6602
Size: 7169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 95 1583 3253 5840 6024 95
Size: 1488 1670 2587 184 1240
Fragments arranged by size:
2587 1670 1488 1240 184
MscI TGG'CCA
Cuts at: 2650 3337 4930 2650
Size: 687 1593 4889
Fragments arranged by size:
4889 1593 687
MslI CAynn'nnrTG
Cuts at: 469 487 1386 2297 2656 3035 3104 3259 5158
Size: 18 899 911 359 379 69 155 1899
Cuts at: 5158 5285 469
Size: 127 2353
Fragments arranged by size:
2353 1899 911 899 379 359 155 127
69 18
MspA1I CmG'CkG
Cuts at: 422 765 867 933 1007 1076 1280 1386 1751
Size: 343 102 66 74 69 204 106 365
Cuts at: 1751 1938 2195 2204 2496 2784 2840 3729 4280
Size: 187 257 9 292 288 56 889 551
Cuts at: 4280 4594 4954 5967 6212 422
Size: 314 360 1013 245 1379
Fragments arranged by size:
1379 1013 889 551 365 360 343 314
292 288 257 245 204 187 106 102
74 69 66 56 9
NaeI GCC'GGC
Cuts at: 1125 3088 5350 1125
Size: 1963 2262 2944
Fragments arranged by size:
2944 2262 1963
NarI GG'CG_CC
Cuts at: 1274 1715 4600 4848 1274
Size: 441 2885 248 3595
Fragments arranged by size:
3595 2885 441 248
NcoI C'CATG_G
Cuts at: 372 398 372
Size: 26 7143
NdeI CA'TA_TG
Cuts at: 2739 2739
Size: 7169
NgoMIV G'CCGG_C
Cuts at: 1123 3086 5348 1123
Size: 1963 2262 2944
Fragments arranged by size:
2944 2262 1963
NspI r_CATG'y
Cuts at: 534 2232 2267 4222 4722 5629 534
Size: 1698 35 1955 500 907 2074
Fragments arranged by size:
2074 1955 1698 907 500 35
PasI CC'CwG_GG
Cuts at: 1841 2051 3922 3996 1841
Size: 210 1871 74 5014
Fragments arranged by size:
5014 1871 210 74
PciI A'CATG_T
Cuts at: 5625 5625
Size: 7169
PflMI CCAn_nnn'nTGG
Cuts at: 2151 3059 2151
Size: 908 6261
PfoI T'CCnGG_A
Cuts at: 629 1481 2828 3305 629
Size: 852 1347 477 4493
Fragments arranged by size:
4493 1347 852 477
PmeI GTTT'AAAC
Cuts at: 4168 4168
Size: 7169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6308 6340 6308
Size: 32 7137
PpuMI rG'GwC_Cy
Cuts at: 678 1548 2172 2859 3342 3444 3570 3741 4074
Size: 870 624 687 483 102 126 171 333
Cuts at: 4074 678
Size: 3773
Fragments arranged by size:
3773 870 687 624 483 333 171 126
102
PspOMI G'GGCC_C
Cuts at: 288 1134 1969 3381 3861 288
Size: 846 835 1412 480 3596
Fragments arranged by size:
3596 1412 846 835 480
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3967 3999 4123 4155 3967
Size: 32 124 32 6981
Fragments arranged by size:
6981 124 32 32
PstI C_TGCA'G
Cuts at: 623 689 821 1993 2042 2474 3799 4216 4901
Size: 66 132 1172 49 432 1325 417 685
Cuts at: 4901 623
Size: 2891
Fragments arranged by size:
2891 1325 1172 685 432 417 132 66
49
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7169
PvuII CAG'CTG
Cuts at: 933 1280 1751 1938 2784 4594 4954 933
Size: 347 471 187 846 1810 360 3148
Fragments arranged by size:
3148 1810 846 471 360 347 187
RsrII CG'GwC_CG
Cuts at: 5364 5364
Size: 7169
SacI G_AGCT'C
Cuts at: 4183 4183
Size: 7169
SacII CC_GC'GG
Cuts at: 2841 2841
Size: 7169
SalI G'TCGA_C
Cuts at: 4206 4206
Size: 7169
SanDI GG'GwC_CC
Cuts at: 1548 3342 1548
Size: 1794 5375
SapI GCTCTTCn'nnn_
Cuts at: 5192 5402 5192
Size: 210 6959
SbfI CC_TGCA'GG
Cuts at: 623 4216 623
Size: 3593 3576
ScaI AGT'ACT
Cuts at: 2036 3985 6686 2036
Size: 1949 2701 2519
Fragments arranged by size:
2701 2519 1949
SexAI A'CCwGG_T
Cuts at: 3446 3446
Size: 7169
SfcI C'TryA_G
Cuts at: 33 619 685 817 1989 2038 2470 3724 3795
Size: 586 66 132 1172 49 432 1254 71
Cuts at: 3795 4212 4897 5890 6081 33
Size: 417 685 993 191 1121
Fragments arranged by size:
1254 1172 1121 993 685 586 432 417
191 132 71 66 49
SfoI GGC'GCC
Cuts at: 1275 1716 4601 4849 1275
Size: 441 2885 248 3595
Fragments arranged by size:
3595 2885 441 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7169
SgrAI Cr'CCGG_yG
Cuts at: 3086 3086
Size: 7169
SmaI CCC'GGG
Cuts at: 262 3184 3509 4191 262
Size: 2922 325 682 3240
Fragments arranged by size:
3240 2922 682 325
SmlI C'TyrA_G
Cuts at: 186 4324 5239 5731 5993 186
Size: 4138 915 492 262 1362
Fragments arranged by size:
4138 1362 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7169
SphI G_CATG'C
Cuts at: 2267 4222 4722 2267
Size: 1955 500 4714
Fragments arranged by size:
4714 1955 500
StuI AGG'CCT
Cuts at: 806 806
Size: 7169
StyI C'CwwG_G
Cuts at: 372 398 2888 3395 3683 4303 4657 372
Size: 26 2490 507 288 620 354 2884
Fragments arranged by size:
2884 2490 620 507 354 288 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 481 727 1828 1990 2305 3112 4113 4752 4885
Size: 246 1101 162 315 807 1001 639 133
Cuts at: 4885 5344 5553 481
Size: 459 209 2097
Fragments arranged by size:
2097 1101 1001 807 639 459 315 246
209 162 133
TatI w'GTAC_w
Cuts at: 2034 2450 3983 6684 2034
Size: 416 1533 2701 2519
Fragments arranged by size:
2701 2519 1533 416
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 374 406 3108 3140 3549 3581 6686 6718 374
Size: 32 2702 32 409 32 3105 32 825
Fragments arranged by size:
3105 2702 825 409 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2824 4966 2824
Size: 2142 5027
XbaI T'CTAG_A
Cuts at: 4200 4200
Size: 7169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1489 2781 3060 3618 4664 1489
Size: 1292 279 558 1046 3994
Fragments arranged by size:
3994 1292 1046 558 279
XmaI C'CCGG_G
Cuts at: 260 3182 3507 4189 260
Size: 2922 325 682 3240
Fragments arranged by size:
3240 2922 682 325
ZraI GAC'GTC
Cuts at: 2354 2354
Size: 7169
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BsrGI Bsu36I ClaI FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI SfiI SnaBI SrfI SspI SwaI XhoI
XmnI