Restriction map for pF1KB9268
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaHI       BseAI       
BsiEI       BsiWI       BsoBI       BspDI       BspEI       
BspMI       BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SwaI        TaqI        Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AcsI         1432  GAATTT               [GA]^AATT[CT]
AflIII         81  ACATGT               A^C[GA][CT]GT
AhdI         1106  GACTCGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         922  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          592  CCTGAGG              CC^T[ACGT]AGG
ApoI         1432  GAATTT               [GA]^AATT[CT]
AspEI        1106  GACTCGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1660  GAGCTC               G[AT]GC[AT]^C
AvaII        1123  GGTCC                G^G[AT]CC
BalI          491  TGGCCA               TGG^CCA
BanII        1660  GAGCTC               G[GA]GC[CT]^C
BcgI          831  CGAAGCTGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         569  GGATAC               GTATCC(6/5)
BfaI         1429  CTAG                 C^TAG
BsaBI         690  GATGTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1693  GAGACC               GGTCTC(1/5)
BsaWI         192  TCCGGT               [AT]^CCGG[AT]
BsgI         1580  CTGCAC               GTGCAG(16/14)
BsiHKAI      1660  GAGCTC               G[AT]GC[AT]^C
BsmBI         900  GAGACG               CGTCTC(1/5)
BsmFI        1124  GTCCC                GGGAC(10/14)
BspLU11I       81  ACATGT               A^CATGT
BstYI          19  GGATCT               [GA]^GATC[CT]
Bsu36I        592  CCTGAGG              CC^T[ACGT]AGG
Cfr13I       1123  GGTCC                G^G[ACGT]CC
Eam1105I     1106  GACTCGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        592  CCTGAGG              CC^T[ACGT]AGG
MaeI         1429  CTAG                 C^TAG
MaeII        1049  ACGT                 A^CGT
MamI          690  GATGTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI           19  GGATCT               [GA]^GATC[CT]
MluNI         491  TGGCCA               TGG^CCA
MscI          491  TGGCCA               TGG^CCA
NcoI          207  CCATGG               C^CATGG
NspI           81  ACATGT               [GA]CATG^[CT]
PshAI          31  GACACTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         1660  GAGCTC               GAGCT^C
SapI          241  GAAGAGC              GCTCTTC(1/4)
Sau96I       1123  GGTCC                G^G[ACGT]CC
SspI          912  AATATT               AAT^ATT
StuI          589  AGGCCT               AGG^CCT
XcmI         1227  CCAGGAGAGTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          19  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AciI          214  GCGG                 CCGC(-3/-1)
              217  GCGG                
AlwI          861  GATCC                GGATC(4/5)
               19  GGATC               
Asp700I       241  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1555  GAAGATTTTC          
BanI          195  GGTGCC               G^G[CT][GA]CC
             1704  GGTGCC              
BbsI           97  GAAGAC               GAAGAC(2/6)
              400  GAAGAC              
BclI         1029  TGATCA               T^GATCA
             1166  TGATCA              
BcnI          476  CCGGG                CC^[GC]GG
              652  CCCGG               
BpuAI          97  GAAGAC               GAAGAC(2/6)
              400  GAAGAC              
Bsp1407I      327  TGTACA               T^GTACA
             1284  TGTACA              
BspHI         159  TCATGA               T^CATGA
              500  TCATGA              
BsrGI         327  TGTACA               T^GTACA
             1284  TGTACA              
BstF5I        465  GGATG                GGATG(2/0)
             1392  GGATG               
BstXI        1179  CCAGCACTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1708  CCAGTGAGCTGG        
DsaI          207  CCATGG               C^C[GA][CT]GG
             1127  CCGTGG              
EarI          241  GAAGAG               CTCTTC(1/4)
             1023  GAAGAG              
FbaI         1029  TGATCA               T^GATCA
             1166  TGATCA              
FokI          465  GGATG                GGATG(9/13)
             1392  GGATG               
HincII         12  GTCAAC               GT[CT]^[GA]AC
              562  GTCAAC              
HindII         12  GTCAAC               GT[CT]^[GA]AC
              562  GTCAAC              
HphI          127  GGTGA                GGTGA(8/7)
              544  GGTGA               
Ksp632I       241  GAAGAG               CTCTTC(1/4)
             1023  GAAGAG              
MslI          172  CACTACTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              538  CATGTTGGTG          
MspA1I         70  CAGCTG               C[AC]G^C[GT]G
             1043  CAGCTG              
NciI          476  CCGGG                CC^[GC]GG
              652  CCCGG               
PstI           73  CTGCAG               CTGCA^G
              515  CTGCAG              
PvuII          70  CAGCTG               CAG^CTG
             1043  CAGCTG              
RcaI          159  TCATGA               T^CATGA
              500  TCATGA              
SfaNI         805  GATGC                GCATC(5/9)
              838  GATGC               
SfcI           73  CTGCAG               C^T[GA][CT]AG
              515  CTGCAG              
SspBI         327  TGTACA               T^GTACA
             1284  TGTACA              
TfiI          156  GATTC                G^A[AT]TC
              497  GAATC               
XmnI          241  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1555  GAAGATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AfaI        AluI        BbvI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmI        
Bsp1286I    BsrI        BssKI       BstNI       Cac8I       
DdeI        DpnII       EaeI        EcoRII      EcoT14I     
Fnu4HI      HaeIII      HapII       HinfI       HpaII       
ItaI        MaeIII      MboI        MboII       MnlI        
MseI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
ScrFI       StyI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
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