Restriction map for pF1KE1010
##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaII       AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoRV       EcoT14I     FbaI        FseI        FspI        
HgaI        Hin1I       HinfI       HpaI        HphI        
KasI        KpnI        KspI        MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspA1I      MunI        MvaI        MvnI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tsp45I      
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AluI          563  AGCT                 AG^CT
ApaI          118  GGGCCC               GGGCC^C
AvaI          218  CTCGGG               C^[CT]CG[GA]G
BanI          277  GGTGCC               G^G[CT][GA]CC
BanII         118  GGGCCC               G[GA]GC[CT]^C
BcgI           76  GCAACGTCGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          414  CCCGG                CC^[GC]GG
BfaI          605  CTAG                 C^TAG
BmyI          118  GGGCCC               G[AGT]GC[ACT]^C
BpmI           49  CTGGAG               CTGGAG(16/14)
BsaJI         217  CCTCGG               C^C[ACGT][ACGT]GG
BsmI          390  GAATGC               GAATGC(1/-1)
BsoBI         218  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      118  GGGCCC               G[AGT]GC[ACT]^C
BsrFI         280  GCCGGC               [GA]^CCGG[CT]
BssKI         414  CCCGG                ^CC[ACGT]GG
CfoI          205  GCGC                 GCG^C
Cfr10I        280  GCCGGC               [GA]^CCGG[CT]
DdeI          587  CTAAG                C^T[ACGT]AG
DpnII         515  GATC                 ^GATC
DraI          367  TTTAAA               TTT^AAA
EcoRI         637  GAATTC               G^AATTC
EcoT22I       392  ATGCAT               ATGCA^T
HaeII         204  AGCGCC               [GA]GCGC^[CT]
HhaI          205  GCGC                 GCG^C
HinP1I        205  GCGC                 G^CGC
HincII        582  GTTGAC               GT[CT]^[GA]AC
HindII        582  GTTGAC               GT[CT]^[GA]AC
HindIII       562  AAGCTT               A^AGCTT
MaeI          605  CTAG                 C^TAG
MaeII          79  ACGT                 A^CGT
MboI          515  GATC                 ^GATC
MslI          288  CATCACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          280  GCCGGC               GCC^GGC
NciI          414  CCCGG                CC^[GC]GG
NdeII         515  GATC                 ^GATC
NgoMIV        280  GCCGGC               G^CCGGC
NsiI          392  ATGCAT               ATGCA^T
NspI          394  GCATGT               [GA]CATG^[CT]
Sau3AI        515  GATC                 ^GATC
ScrFI         414  CCCGG                CC^[ACGT]GG
SmlI          303  CTTGAG               C^T[CT][GA]AG
TaqI           32  TCGA                 T^CGA
TthHB8I        32  TCGA                 T^CGA
XcmI           38  CCATCTCGGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BsiYI         212  CCCCGCCTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              213  CCCGCCTCGGG         
BslI          212  CCCCGCCTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              213  CCCGCCTCGGG         
BsmAI         446  GTCTC                GTCTC(1/5)
              494  GTCTC               
BstF5I          6  CATCC                GGATG(2/0)
              138  GGATG               
Cac8I         280  GCCGGC               GC[ACGT]^[ACGT]GC
              564  GCTTGC              
EaeI          282  CGGCCA               [CT]^GGCC[GA]
              381  TGGCCG              
FauI          213  CCCGC                CCCGC(4/6)
              273  GCGGG               
FokI            6  CATCC                GGATG(9/13)
              138  GGATG               
HapII         281  CCGG                 C^CGG
              415  CCGG                
HpaII         281  CCGG                 C^CGG
              415  CCGG                
MseI          368  TTAA                 T^TAA
              529  TTAA                
MspI          281  CCGG                 C^CGG
              415  CCGG                
NlaIII         21  CATG                 CATG^
              395  CATG                
SfaNI           5  GCATC                GCATC(5/9)
              237  GCATC               
Tru9I         368  TTAA                 T^TAA
              529  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AciI        AcsI        ApoI        BbvI        BseRI       
BsrI        Cfr13I      EarI        Fnu4HI      HaeIII      
ItaI        Ksp632I     MboII       MnlI        MwoI        
NlaIV       Sau96I      TseI        Tsp509I     TspRI       
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