Restriction map for pF1KE1059
##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AlwI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     BamHI       
BbrPI       BcgI        BciVI       BclI        BfrI        
BsaAI       BsaBI       BsaWI       BseAI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrGI       BssHII      BstBI       
ClaI        DraI        DraIII      DrdI        EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HpaI        
KpnI        MamI        MfeI        MluI        MroI        
MseI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        ScaI        SfiI        
SfuI        SnaBI       SpeI        SspBI       SspI        
SwaI        TfiI        Tru9I       Tsp509I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AflIII       1079  ACGTGT               A^C[GA][CT]GT
AhdI         2389  GACACACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1651  GTGCAC               G^TGCAC
Aor51HI        27  AGCGCT               AGC^GCT
ApaI         2209  GGGCCC               GGGCC^C
ApaLI        1651  GTGCAC               G^TGCAC
AspEI        2389  GACACACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        2423  TGCGCA               TGC^GCA
AvrII        2589  CCTAGG               C^CTAGG
BbeI          149  GGCGCC               GGCGC^C
BbsI         2028  GAAGAC               GAAGAC(2/6)
BfaI         2590  CTAG                 C^TAG
BlnI         2589  CCTAGG               C^CTAGG
BpuAI        2028  GAAGAC               GAAGAC(2/6)
BsiEI        1070  CGGCCG               CG[GA][CT]^CG
BspHI          86  TCATGA               T^CATGA
BssSI        1546  CACGAG               CACGAG(-5/-1)
Bst1107I      667  GTATAC               GTA^TAC
BstEII       2433  GGTGACC              G^GT[ACGT]ACC
BstPI        2433  GGTGACC              G^GT[ACGT]ACC
BstZ17I       667  GTATAC               GTA^TAC
CpoI         2216  CGGACCG              CG^G[AT]CCG
EagI         1070  CGGCCG               C^GGCCG
Eam1105I     2389  GACACACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1070  CGGCCG               C^GGCCG
Eco47III       27  AGCGCT               AGC^GCT
Eco52I       1070  CGGCCG               C^GGCCG
EcoNI         489  CCTGTACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2433  GGTGACC              G^GT[ACGT]ACC
FspI         2423  TGCGCA               TGC^GCA
HincII       1213  GTCGAC               GT[CT]^[GA]AC
HindII       1213  GTCGAC               GT[CT]^[GA]AC
HindIII      1618  AAGCTT               A^AGCTT
KasI          149  GGCGCC               G^GCGCC
KspI         2522  CCGCGG               CCGC^GG
MaeI         2590  CTAG                 C^TAG
NarI          149  GGCGCC               GG^CGCC
RcaI           86  TCATGA               T^CATGA
RsrII        2216  CGGACCG              CG^G[AT]CCG
SacI           61  GAGCTC               GAGCT^C
SacII        2522  CCGCGG               CCGC^GG
SalI         1213  GTCGAC               G^TCGAC
SbfI         1509  CCTGCAGG             CCTGCA^GG
SfoI          149  GGCGCC               GGC^GCC
SgrAI        1449  CGCCGGTG             C[GA]^CCGG[CT]G
SphI         1611  GCATGC               GCATG^C
Sse8387I     1509  CCTGCAGG             CCTGCA^GG
StuI          375  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AocI         1189  CCTGAGG              CC^T[ACGT]AGG
             1843  CCTGAGG             
AspHI          61  GAGCTC               G[AT]GC[AT]^C
             1651  GTGCAC              
AspI          334  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1993  GACTACGTC           
BanII          61  GAGCTC               G[GA]GC[CT]^C
             2209  GGGCCC              
BbvCI         253  CCTCAGC              CCTCAGC(-5/-2)
             2358  CCTCAGC             
BglII         182  AGATCT               A^GATCT
             2327  AGATCT              
BlpI          367  GCTCAGC              GC^T[ACGT]AGC
             1620  GCTTAGC             
Bpu1102I      367  GCTCAGC              GC^T[ACGT]AGC
             1620  GCTTAGC             
BsaI          839  GAGACC               GGTCTC(1/5)
              973  GAGACC              
BseRI         970  GAGGAG               GAGGAG(10/8)
             2017  GAGGAG              
BsiHKAI        61  GAGCTC               G[AT]GC[AT]^C
             1651  GTGCAC              
BsrDI        1580  GCAATG               GCAATG(2/0)
             1796  GCAATG              
BstAPI       1503  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2223  GCAGCTGCTGC         
BstYI         182  AGATCT               [GA]^GATC[CT]
             2327  AGATCT              
Bsu36I       1189  CCTGAGG              CC^T[ACGT]AGG
             1843  CCTGAGG             
CelII         367  GCTCAGC              GC^T[ACGT]AGC
             1620  GCTTAGC             
DpnII         183  GATC                 ^GATC
             2328  GATC                
EarI         1810  CTCTTC               CTCTTC(1/4)
             2233  CTCTTC              
Eco81I       1189  CCTGAGG              CC^T[ACGT]AGG
             1843  CCTGAGG             
Ksp632I      1810  CTCTTC               CTCTTC(1/4)
             2233  CTCTTC              
MboI          183  GATC                 ^GATC
             2328  GATC                
MflI          182  AGATCT               [GA]^GATC[CT]
             2327  AGATCT              
NaeI         1068  GCCGGC               GCC^GGC
             1625  GCCGGC              
NcoI          590  CCATGG               C^CATGG
              812  CCATGG              
NdeII         183  GATC                 ^GATC
             2328  GATC                
NgoMIV       1068  GCCGGC               G^CCGGC
             1625  GCCGGC              
NspI          194  GCATGT               [GA]CATG^[CT]
             1611  GCATGC              
PflFI         334  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1993  GACTACGTC           
SapI         1809  GCTCTTC              GCTCTTC(1/4)
             2232  GCTCTTC             
Sau3AI        183  GATC                 ^GATC
             2328  GATC                
SmlI          178  CTCAAG               C^T[CT][GA]AG
             1717  CTTGAG              
Tth111I       334  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1993  GACTACGTC           
XcmI          249  CCAGCCTCAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2324  CCAAGATCTTTGTGG     
XhoII         182  AGATCT               [GA]^GATC[CT]
             2327  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwNI       AvaI        AvaII       BalI        
BanI        BbvI        BcnI        BglI        BmyI        
BpmI        BsaHI       BsaJI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NlaIII      NlaIV       PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmaI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     Van91I      XmaCI       XmaI        
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