Restriction map for pF1KE1059
 (Linear) (Six-base) MAPSORT of: KIEE1059.seq  Check: 8396  from: 1  to: 2610



KIEE1059.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     725    2610 
   Size:        725    1885
 
AccI GT'mk_AC

Cuts at:      0     341     668     914    1214    2610 
   Size:        341     327     246     300    1396

  Fragments arranged by size: 

                1396     341     327     300     246
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1797    2122    2610 
   Size:       1797     325     488

  Fragments arranged by size: 

                1797     488     325
 
AfeI AGC'GCT

Cuts at:      0      29    2610 
   Size:         29    2581
 
AflIII A'CryG_T

Cuts at:      0    1079    2610 
   Size:       1079    1531
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2394    2610 
   Size:       2394     216
 
AleI CACnn'nnGTG

Cuts at:      0     649    2610 
   Size:        649    1961
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     961    1814    2610 
   Size:        961     853     796

  Fragments arranged by size: 

                 961     853     796
 
AlwNI CAG_nnn'CTG

Cuts at:      0     117     220     511    1509    1662    2100    2229    2610 
   Size:        117     103     291     998     153     438     129     381

  Fragments arranged by size: 

                 998     438     381     291     153     129     117     103
            
 
ApaI G_GGCC'C

Cuts at:      0    2213    2610 
   Size:       2213     397
 
ApaLI G'TGCA_C

Cuts at:      0    1651    2610 
   Size:       1651     959
 
AvaI C'yCGr_G

Cuts at:      0     390     726    1146    1759    1836    2187    2610 
   Size:        390     336     420     613      77     351     423

  Fragments arranged by size: 

                 613     423     420     390     351     336      77
 
AvrII C'CTAG_G

Cuts at:      0    2589    2610 
   Size:       2589      21
 
BanI G'GyrC_C

Cuts at:      0     149     348     498     540     639    1150    1233    1855 
   Size:        149     199     150      42      99     511      83     622

Cuts at:   1855    2610 
   Size:        755

  Fragments arranged by size: 

                 755     622     511     199     150     149      99      83
                  42
 
BanII G_rGCy'C

Cuts at:      0      65    2213    2610 
   Size:         65    2148     397

  Fragments arranged by size: 

                2148     397      65
 
BbeI G_GCGC'C

Cuts at:      0     153    2610 
   Size:        153    2457
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2035    2610 
   Size:       2035     575
 
BbvCI CC'TCA_GC

Cuts at:      0     254    2359    2610 
   Size:        254    2105     251

  Fragments arranged by size: 

                2105     254     251
 
BglI GCCn_nnn'nGGC

Cuts at:      0     122     216     244     507     785    1700    1950    2610 
   Size:        122      94      28     263     278     915     250     660

  Fragments arranged by size: 

                 915     660     278     263     250     122      94      28
            
 
BglII A'GATC_T

Cuts at:      0     182    2327    2610 
   Size:        182    2145     283

  Fragments arranged by size: 

                2145     283     182
 
BlpI GC'TnA_GC

Cuts at:      0     368    1621    2610 
   Size:        368    1253     989

  Fragments arranged by size: 

                1253     989     368
 
Bme1580I G_kGCm'C

Cuts at:      0     353     503     616     644     755     869     950     989 
   Size:        353     150     113      28     111     114      81      39

Cuts at:    989    1124    1153    1169    1608    1655    1760    2213    2610 
   Size:        135      29      16     439      47     105     453     397

  Fragments arranged by size: 

                 453     439     397     353     150     135     114     113
                 111     105      81      47      39      29      28      16
            
 
BmgBI CAC'GTC

Cuts at:      0     864    1080    1089    1119    1650    2421    2610 
   Size:        864     216       9      30     531     771     189

  Fragments arranged by size: 

                 864     771     531     216     189      30       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     285     866    1469    2610 
   Size:        285     581     603    1141

  Fragments arranged by size: 

                1141     603     581     285
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     533     871    1040    1272    1381    1727    2107 
   Size:        118     415     338     169     232     109     346     380

Cuts at:   2107    2263    2610 
   Size:        156     347

  Fragments arranged by size: 

                 415     380     347     346     338     232     169     156
                 118     109
 
Bpu10I CC'TnA_GC

Cuts at:      0     254    2359    2610 
   Size:        254    2105     251

  Fragments arranged by size: 

                2105     254     251
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163    1738    2610 
   Size:        163    1575     872

  Fragments arranged by size: 

                1575     872     163
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     833     967    2610 
   Size:        833     134    1643

  Fragments arranged by size: 

                1643     833     134
 
BsaHI Gr'CG_yC

Cuts at:      0     150    1766    2167    2288    2610 
   Size:        150    1616     401     121     322

  Fragments arranged by size: 

                1616     401     322     150     121
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1455    1485    2610 
   Size:       1455      30    1125

  Fragments arranged by size: 

                1455    1125      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     985    2032    2610 
   Size:        985    1047     578

  Fragments arranged by size: 

                1047     985     578
 
BseYI C'CCAG_C

Cuts at:      0     248     273     326     358     397     420     472     605 
   Size:        248      25      53      32      39      23      52     133

Cuts at:    605     680     775    1242    1260    2556    2570    2610 
   Size:         75      95     467      18    1296      14      40

  Fragments arranged by size: 

                1296     467     248     133      95      75      53      52
                  40      39      32      25      23      18      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1522    1774    1846    2610 
   Size:       1522     252      72     764

  Fragments arranged by size: 

                1522     764     252      72
 
BsiEI CG_ry'CG

Cuts at:      0    1073    2610 
   Size:       1073    1537
 
BsiHKAI G_wGCw'C

Cuts at:      0      65    1655    2610 
   Size:         65    1590     955

  Fragments arranged by size: 

                1590     955      65
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     353     503     616     644     755     869     950 
   Size:         65     288     150     113      28     111     114      81

Cuts at:    950     989    1124    1153    1169    1608    1655    1760    2213 
   Size:         39     135      29      16     439      47     105     453

Cuts at:   2213    2610 
   Size:        397

  Fragments arranged by size: 

                 453     439     397     288     150     135     114     113
                 111     105      81      65      47      39      29      28
                  16
 
BspHI T'CATG_A

Cuts at:      0      86    2610 
   Size:         86    2524
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     725    1517    2459    2610 
   Size:        725     792     942     151

  Fragments arranged by size: 

                 942     792     725     151
 
BsrDI GCAATG_nn'

Cuts at:      0    1587    1803    2610 
   Size:       1587     216     807

  Fragments arranged by size: 

                1587     807     216
 
BsrFI r'CCGG_y

Cuts at:      0    1068    1294    1450    1625    1730    2219    2610 
   Size:       1068     226     156     175     105     489     391

  Fragments arranged by size: 

                1068     489     391     226     175     156     105
 
BssSI C'ACGA_G

Cuts at:      0    1546    2610 
   Size:       1546    1064
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1509    2229    2610 
   Size:       1509     720     381

  Fragments arranged by size: 

                1509     720     381
 
BstEII G'GTnAC_C

Cuts at:      0    2433    2610 
   Size:       2433     177
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     651    1776    2283    2610 
   Size:        651    1125     507     327

  Fragments arranged by size: 

                1125     651     507     327
 
BstYI r'GATC_y

Cuts at:      0     182    2327    2610 
   Size:        182    2145     283

  Fragments arranged by size: 

                2145     283     182
 
BstZ17I GTA'TAC

Cuts at:      0     669    2610 
   Size:        669    1941
 
Bsu36I CC'TnA_GG

Cuts at:      0    1190    1844    2610 
   Size:       1190     654     766

  Fragments arranged by size: 

                1190     766     654
 
BtgI C'CryG_G

Cuts at:      0     590     812    2282    2303    2522    2610 
   Size:        590     222    1470      21     219      88

  Fragments arranged by size: 

                1470     590     222     219      88      21
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2498    2610 
   Size:       2498     112
 
BtsI GCAGTG_nn'

Cuts at:      0     958    1804    1829    2254    2610 
   Size:        958     846      25     425     356

  Fragments arranged by size: 

                 958     846     425     356      25
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     617     652    2610 
   Size:        617      35    1958

  Fragments arranged by size: 

                1958     617      35
 
EaeI y'GGCC_r

Cuts at:      0      20      44     479     786     834    1070    1523    1692 
   Size:         20      24     435     307      48     236     453     169

Cuts at:   1692    2156    2306    2480    2610 
   Size:        464     150     174     130

  Fragments arranged by size: 

                 464     453     435     307     236     174     169     150
                 130      48      24      20
 
EagI C'GGCC_G

Cuts at:      0    1070    2610 
   Size:       1070    1540
 
EarI CTCTTCn'nnn_

Cuts at:      0    1816    2239    2610 
   Size:       1816     423     371

  Fragments arranged by size: 

                1816     423     371
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2569    2610 
   Size:       2569      41
 
EcoICRI GAG'CTC

Cuts at:      0      63    2610 
   Size:         63    2547
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     533     871    1040    1272    1381    1727    1797 
   Size:        118     415     338     169     232     109     346      70

Cuts at:   1797    2107    2122    2263    2610 
   Size:        310      15     141     347

  Fragments arranged by size: 

                 415     347     346     338     310     232     169     141
                 118     109      70      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     493    2610 
   Size:        493    2117
 
EcoO109I rG'GnC_Cy

Cuts at:      0     114     217     394     508     517     579     826     885 
   Size:        114     103     177     114       9      62     247      59

Cuts at:    885    1383    1506    1680    1722    1840    2209    2210    2610 
   Size:        498     123     174      42     118     369       1     400

  Fragments arranged by size: 

                 498     400     369     247     177     174     123     118
                 114     114     103      62      59      42       9       1
            
 
FspI TGC'GCA

Cuts at:      0    2425    2610 
   Size:       2425     185
 
HaeII r_GCGC'y

Cuts at:      0      31     153     280    1278    1415    1451    2366    2610 
   Size:         31     122     127     998     137      36     915     244

  Fragments arranged by size: 

                 998     915     244     137     127     122      36      31
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      49      81     444     476    2380    2412    2610 
   Size:         49      32     363      32    1904      32     198

  Fragments arranged by size: 

                1904     363     198      49      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1215    2610 
   Size:       1215    1395
 
HindIII A'AGCT_T

Cuts at:      0    1618    2610 
   Size:       1618     992
 
KasI G'GCGC_C

Cuts at:      0     149    2610 
   Size:        149    2461
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     923    1535    2610 
   Size:        923     612    1075

  Fragments arranged by size: 

                1075     923     612
 
MscI TGG'CCA

Cuts at:      0      22     788    2158    2308    2482    2610 
   Size:         22     766    1370     150     174     128

  Fragments arranged by size: 

                1370     766     174     150     128      22
 
MslI CAynn'nnrTG

Cuts at:      0      91     301     649    1796    2610 
   Size:         91     210     348    1147     814

  Fragments arranged by size: 

                1147     814     348     210      91
 
MspA1I CmG'CkG

Cuts at:      0     605    1246    1497    1632    1776    2085    2100    2226 
   Size:        605     641     251     135     144     309      15     126

Cuts at:   2226    2497    2524    2598    2610 
   Size:        271      27      74      12

  Fragments arranged by size: 

                 641     605     309     271     251     144     135     126
                  74      27      15      12
 
NaeI GCC'GGC

Cuts at:      0    1070    1627    2610 
   Size:       1070     557     983

  Fragments arranged by size: 

                1070     983     557
 
NarI GG'CG_CC

Cuts at:      0     150    2610 
   Size:        150    2460
 
NcoI C'CATG_G

Cuts at:      0     590     812    2610 
   Size:        590     222    1798

  Fragments arranged by size: 

                1798     590     222
 
NgoMIV G'CCGG_C

Cuts at:      0    1068    1625    2610 
   Size:       1068     557     985

  Fragments arranged by size: 

                1068     985     557
 
NspI r_CATG'y

Cuts at:      0     198    1615    2610 
   Size:        198    1417     995

  Fragments arranged by size: 

                1417     995     198
 
PasI CC'CwG_GG

Cuts at:      0     505    2610 
   Size:        505    2105
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     511    1431    1662    2154    2181    2373    2535    2610 
   Size:        511     920     231     492      27     192     162      75

  Fragments arranged by size: 

                 920     511     492     231     192     162      75      27
            
 
PpuMI rG'GwC_Cy

Cuts at:      0     394     579     826    1506    1722    2610 
   Size:        394     185     247     680     216     888

  Fragments arranged by size: 

                 888     680     394     247     216     185
 
PspOMI G'GGCC_C

Cuts at:      0    2209    2610 
   Size:       2209     401
 
PstI C_TGCA'G

Cuts at:      0    1400    1514    1637    2610 
   Size:       1400     114     123     973

  Fragments arranged by size: 

                1400     973     123     114
 
PvuII CAG'CTG

Cuts at:      0     605    1246    1632    1776    2085    2100    2226    2598 
   Size:        605     641     386     144     309      15     126     372

Cuts at:   2598    2610 
   Size:         12

  Fragments arranged by size: 

                 641     605     386     372     309     144     126      15
                  12
 
RsrII CG'GwC_CG

Cuts at:      0    2217    2610 
   Size:       2217     393
 
SacI G_AGCT'C

Cuts at:      0      65    2610 
   Size:         65    2545
 
SacII CC_GC'GG

Cuts at:      0    2525    2610 
   Size:       2525      85
 
SalI G'TCGA_C

Cuts at:      0    1213    2610 
   Size:       1213    1397
 
SanDI GG'GwC_CC

Cuts at:      0     394     826    2610 
   Size:        394     432    1784

  Fragments arranged by size: 

                1784     432     394
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1816    2239    2610 
   Size:       1816     423     371

  Fragments arranged by size: 

                1816     423     371
 
SbfI CC_TGCA'GG

Cuts at:      0    1514    2610 
   Size:       1514    1096
 
SexAI A'CCwGG_T

Cuts at:      0     344     494     536     575    2610 
   Size:        344     150      42      39    2035

  Fragments arranged by size: 

                2035     344     150      42      39
 
SfcI C'TryA_G

Cuts at:      0    1396    1510    1633    2005    2442    2610 
   Size:       1396     114     123     372     437     168

  Fragments arranged by size: 

                1396     437     372     168     123     114
 
SfoI GGC'GCC

Cuts at:      0     151    2610 
   Size:        151    2459
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1450    2610 
   Size:       1450    1160
 
SmaI CCC'GGG

Cuts at:      0     392     728    1148    1838    2189    2610 
   Size:        392     336     420     690     351     421

  Fragments arranged by size: 

                 690     421     420     392     351     336
 
SmlI C'TyrA_G

Cuts at:      0     178    1717    2610 
   Size:        178    1539     893

  Fragments arranged by size: 

                1539     893     178
 
SphI G_CATG'C

Cuts at:      0    1615    2610 
   Size:       1615     995
 
SrfI GCCC'GGGC

Cuts at:      0    1148    2610 
   Size:       1148    1462
 
StuI AGG'CCT

Cuts at:      0     377    2610 
   Size:        377    2233
 
StyI C'CwwG_G

Cuts at:      0     590     812    1001    1328    1823    2408    2489    2589 
   Size:        590     222     189     327     495     585      81     100

Cuts at:   2589    2610 
   Size:         21

  Fragments arranged by size: 

                 590     585     495     327     222     189     100      81
                  21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2584    2610 
   Size:       2584      26
 
TatI w'GTAC_w

Cuts at:      0     293    2610 
   Size:        293    2317
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     112     144    2610 
   Size:        112      32    2466

  Fragments arranged by size: 

                2466     112      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     337    1996    2610 
   Size:        337    1659     614

  Fragments arranged by size: 

                1659     614     337
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     256    2331    2610 
   Size:        256    2075     279

  Fragments arranged by size: 

                2075     279     256
 
XmaI C'CCGG_G

Cuts at:      0     390     726    1146    1836    2187    2610 
   Size:        390     336     420     690     351     423

  Fragments arranged by size: 

                 690     423     420     390     351     336
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI
    AlwNI     ApaI    ApaLI     AvaI    AvrII     BanI    BanII     BbeI
     BbsI    BbvCI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI
    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI    CspCI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI    PflMI    PpuMI   PspOMI     PstI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfcI     SfoI    SgrAI     SmaI     SmlI     SphI     SrfI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AloI     ApoI     AscI
     AseI    AsiSI     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI
     BmtI     BplI    BsaAI    BsaBI    BsaWI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrGI   BssHII    BstBI     ClaI     DraI   DraIII
     DrdI    EcoRI    EcoRV     FalI     FseI    FspAI     HpaI     KpnI
     MfeI     MluI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI     ScaI     SfiI     SgfI    SnaBI     SpeI     SspI
     SwaI     XbaI     XhoI     XmnI     ZraI
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