Restriction map for pF1KB6010
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AgeI        Alw44I      AlwNI       Aor51HI     
ApaLI       AscI        Asp718I     AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvCI       BfrI        BglI        BglII       
BlnI        BsaAI       BsaHI       BsaI        BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspMI       
BsrBI       BssHII      BssSI       BstAPI      BstEII      
BstPI       BstUI       CfoI        ClaI        CpoI        
EagI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     FauI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HpaI        KasI        KpnI        KspI        MluI        
MluNI       MscI        MvnI        NaeI        NarI        
NgoMIV      NotI        NruI        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SwaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccI         2442  GTATAC               GT^[AC][GT]AC
AccIII       1847  TCCGGA               T^CCGGA
AflIII       2423  ACATGT               A^C[GA][CT]GT
AhdI         3163  GACACCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3400  CCTGAGG              CC^T[ACGT]AGG
ApaI          144  GGGCCC               GGGCC^C
AseI          715  ATTAAT               AT^TAAT
AsnI          715  ATTAAT               AT^TAAT
AspEI        3163  GACACCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1556  GTGCTC               G[AT]GC[AT]^C
AspI         1358  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcgI          913  CGAGCTATTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         591  GGATAC               GTATCC(6/5)
BclI         2883  TGATCA               T^GATCA
BcnI         3195  CCGGG                CC^[GC]GG
BlpI         2475  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2475  GCTCAGC              GC^T[ACGT]AGC
BsaWI        1847  TCCGGA               [AT]^CCGG[AT]
BseAI        1847  TCCGGA               T^CCGGA
BsiHKAI      1556  GTGCTC               G[AT]GC[AT]^C
BsmAI        2168  GAGAC                GTCTC(1/5)
BsmI          855  GAATGC               GAATGC(1/-1)
Bsp1407I      879  TGTACA               T^GTACA
BspEI        1847  TCCGGA               T^CCGGA
BspLU11I     2423  ACATGT               A^CATGT
BsrGI         879  TGTACA               T^GTACA
Bst1107I     2442  GTATAC               GTA^TAC
BstBI         434  TTCGAA               TT^CGAA
BstNI         660  CCTGG                CC^[AT]GG
BstXI         222  CCAACAGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2442  GTATAC               GTA^TAC
Bsu36I       3400  CCTGAGG              CC^T[ACGT]AGG
CelII        2475  GCTCAGC              GC^T[ACGT]AGC
DraII         143  AGGGCCC              [GA]G^G[ACGT]CC[CT]
DraIII       1517  CACTTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1264  CGGCCA               [CT]^GGCC[GA]
Eam1105I     3163  GACACCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3400  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      143  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRII        660  CCTGG                ^CC[AT]GG
FbaI         2883  TGATCA               T^GATCA
MfeI         3506  CAATTG               C^AATTG
MroI         1847  TCCGGA               T^CCGGA
MunI         3506  CAATTG               C^AATTG
MvaI          660  CCTGG                CC^[AT]GG
NciI         3195  CCGGG                CC^[GC]GG
NdeI          412  CATATG               CA^TATG
NheI         3144  GCTAGC               G^CTAGC
NlaIV         144  GGGCCC               GG[ACGT]^[ACGT]CC
NspV          434  TTCGAA               TT^CGAA
PflFI        1358  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1516  CCACTTAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI         715  ATTAAT               AT^TAAT
ScaI         1778  AGTACT               AGT^ACT
SfuI          434  TTCGAA               TT^CGAA
SspBI         879  TGTACA               T^GTACA
StuI         2357  AGGCCT               AGG^CCT
Tth111I      1358  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1516  CCACTTAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1404  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BanII         144  GGGCCC               G[GA]GC[CT]^C
             3382  GAGCCC              
BpmI           11  CTCCAG               CTGGAG(16/14)
             1283  CTGGAG              
BseRI         176  GAGGAG               GAGGAG(10/8)
             1327  GAGGAG              
BsmFI        1454  GGGAC                GGGAC(10/14)
             1624  GGGAC               
BspHI        3074  TCATGA               T^CATGA
             3413  TCATGA              
BsrFI        1262  ACCGGC               [GA]^CCGG[CT]
             1997  ACCGGC              
BssKI         660  CCTGG                ^CC[ACGT]GG
             3195  CCGGG               
Cfr10I       1262  ACCGGC               [GA]^CCGG[CT]
             1997  ACCGGC              
DrdI          625  GACTCACAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1031  GACTCAAATGTC        
EcoRI         451  GAATTC               G^AATTC
              529  GAATTC              
EcoT14I       485  CCATGG               C^C[AT][AT]GG
             3578  CCATGG              
EcoT22I      1006  ATGCAT               ATGCA^T
             2048  ATGCAT              
MaeII        2333  ACGT                 A^CGT
             2859  ACGT                
MspA1I       1280  CAGCTG               C[AC]G^C[GT]G
             2642  CAGCTG              
NcoI          485  CCATGG               C^CATGG
             3578  CCATGG              
NsiI         1006  ATGCAT               ATGCA^T
             2048  ATGCAT              
PvuII        1280  CAGCTG               CAG^CTG
             2642  CAGCTG              
RcaI         3074  TCATGA               T^CATGA
             3413  TCATGA              
ScrFI         660  CCTGG                CC^[ACGT]GG
             3195  CCGGG               
SphI         1425  GCATGC               GCATG^C
             3260  GCATGC              
SspI          861  AATATT               AAT^ATT
             1852  AATATT              
StyI          485  CCATGG               C^C[AT][AT]GG
             3578  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AvaII       BbsI        BbvI        
BfaI        BmyI        BpuAI       BsaBI       BsaJI       
BsgI        BsiYI       BslI        Bsp1286I    BsrDI       
BsrI        BstF5I      BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        DsaI        EarI        
EcoRV       Fnu4HI      FokI        HaeIII      HapII       
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MamI        
MboI        MboII       MflI        MnlI        MseI        
MslI        MspI        MwoI        NdeII       NlaIII      
NspI        PleI        PstI        RsaI        SapI        
Sau3AI      Sau96I      SfaNI       SfcI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com