Restriction map for pF1KB6010
 (Linear) (Six-base) MAPSORT of: KIBB6010.seq  Check: 4451  from: 1  to: 3693



KIBB6010.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AccI GT'mk_AC

Cuts at:      0    2443    3693 
   Size:       2443    1250
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     467     805    1293    2376    3299    3649    3693 
   Size:        467     338     488    1083     923     350      44

  Fragments arranged by size: 

                1083     923     488     467     350     338      44
 
AflIII A'CryG_T

Cuts at:      0    2423    3693 
   Size:       2423    1270
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3168    3693 
   Size:       3168     525
 
AleI CACnn'nnGTG

Cuts at:      0    2202    3693 
   Size:       2202    1491
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1571    1844    3693 
   Size:       1571     273    1849

  Fragments arranged by size: 

                1849    1571     273
 
ApaI G_GGCC'C

Cuts at:      0     148    3693 
   Size:        148    3545
 
ApoI r'AATT_y

Cuts at:      0     451     529     908    1288    1990    2586    2735    2935 
   Size:        451      78     379     380     702     596     149     200

Cuts at:   2935    2985    3013    3135    3693 
   Size:         50      28     122     558

  Fragments arranged by size: 

                 702     596     558     451     380     379     200     149
                 122      78      50      28
 
AseI AT'TA_AT

Cuts at:      0     716    3693 
   Size:        716    2977
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1865    1898    3693 
   Size:       1865      33    1795

  Fragments arranged by size: 

                1865    1795      33
 
BanII G_rGCy'C

Cuts at:      0     148    3386    3693 
   Size:        148    3238     307

  Fragments arranged by size: 

                3238     307     148
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1362    1666    2490    3693 
   Size:       1362     304     824    1203

  Fragments arranged by size: 

                1362    1203     824     304
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     902     936    3693 
   Size:        902      34    2757

  Fragments arranged by size: 

                2757     902      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     585    3693 
   Size:        585    3108
 
BclI T'GATC_A

Cuts at:      0    2883    3693 
   Size:       2883     810
 
BfrBI ATG'CAT

Cuts at:      0    1008    2050    3693 
   Size:       1008    1042    1643

  Fragments arranged by size: 

                1643    1042    1008
 
BlpI GC'TnA_GC

Cuts at:      0    2476    3693 
   Size:       2476    1217
 
Bme1580I G_kGCm'C

Cuts at:      0     148    3693 
   Size:        148    3545
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1491    3693 
   Size:       1491    2202
 
BmtI G_CTAG'C

Cuts at:      0    3148    3693 
   Size:       3148     545
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1309    3693 
   Size:       1309    2384
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1304    3693 
   Size:       1304    2389
 
Bpu10I CC'TnA_GC

Cuts at:      0    2177    3693 
   Size:       2177    1516
 
BsaBI GATnn'nnATC

Cuts at:      0      32    2051    3464    3693 
   Size:         32    2019    1413     229

  Fragments arranged by size: 

                2019    1413     229      32
 
BsaWI w'CCGG_w

Cuts at:      0    1847    3693 
   Size:       1847    1846
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     863     893    3693 
   Size:        863      30    2800

  Fragments arranged by size: 

                2800     863      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     191    1342    3693 
   Size:        191    1151    2351

  Fragments arranged by size: 

                2351    1151     191
 
BseYI C'CCAG_C

Cuts at:      0     147    3693 
   Size:        147    3546
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2549    2557    3313    3693 
   Size:       2549       8     756     380

  Fragments arranged by size: 

                2549     756     380       8
 
BsiHKAI G_wGCw'C

Cuts at:      0    1560    3693 
   Size:       1560    2133
 
BsmI GAATG_Cn'

Cuts at:      0     861    3693 
   Size:        861    2832
 
Bsp1286I G_dGCh'C

Cuts at:      0     148    1560    3386    3693 
   Size:        148    1412    1826     307

  Fragments arranged by size: 

                1826    1412     307     148
 
BspEI T'CCGG_A

Cuts at:      0    1847    3693 
   Size:       1847    1846
 
BspHI T'CATG_A

Cuts at:      0    3074    3413    3693 
   Size:       3074     339     280

  Fragments arranged by size: 

                3074     339     280
 
BsrDI GCAATG_nn'

Cuts at:      0     559    2458    2906    3693 
   Size:        559    1899     448     787

  Fragments arranged by size: 

                1899     787     559     448
 
BsrFI r'CCGG_y

Cuts at:      0    1262    1997    3693 
   Size:       1262     735    1696

  Fragments arranged by size: 

                1696    1262     735
 
BsrGI T'GTAC_A

Cuts at:      0     879    3693 
   Size:        879    2814
 
BstBI TT'CG_AA

Cuts at:      0     435    3693 
   Size:        435    3258
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     229    3693 
   Size:        229    3464
 
BstYI r'GATC_y

Cuts at:      0    1320    1844    3549    3693 
   Size:       1320     524    1705     144

  Fragments arranged by size: 

                1705    1320     524     144
 
BstZ17I GTA'TAC

Cuts at:      0    2444    3693 
   Size:       2444    1249
 
Bsu36I CC'TnA_GG

Cuts at:      0    3401    3693 
   Size:       3401     292
 
BtgI C'CryG_G

Cuts at:      0     485    1613    1703    3578    3693 
   Size:        485    1128      90    1875     115

  Fragments arranged by size: 

                1875    1128     485     115      90
 
BtsI GCAGTG_nn'

Cuts at:      0    1214    2081    2197    2209    3325    3693 
   Size:       1214     867     116      12    1116     368

  Fragments arranged by size: 

                1214    1116     867     368     116      12
 
DraI TTT'AAA

Cuts at:      0     867     986    2282    2946    2984    3693 
   Size:        867     119    1296     664      38     709

  Fragments arranged by size: 

                1296     867     709     664     119      38
 
DraIII CAC_nnn'GTG

Cuts at:      0    1522    3693 
   Size:       1522    2171
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     631    1037    3693 
   Size:        631     406    2656

  Fragments arranged by size: 

                2656     631     406
 
EaeI y'GGCC_r

Cuts at:      0    1264    3693 
   Size:       1264    2429
 
EarI CTCTTCn'nnn_

Cuts at:      0     923    1312    1344    1505    1614    1919    2068    3093 
   Size:        923     389      32     161     109     305     149    1025

Cuts at:   3093    3494    3693 
   Size:        401     199

  Fragments arranged by size: 

                1025     923     401     389     305     199     161     149
                 109      32
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     467     805    1293    1304    2376    3299    3649    3693 
   Size:        467     338     488      11    1072     923     350      44

  Fragments arranged by size: 

                1072     923     488     467     350     338      44      11
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     144    3693 
   Size:        144    3549
 
EcoRI G'AATT_C

Cuts at:      0     451     529    3693 
   Size:        451      78    3164

  Fragments arranged by size: 

                3164     451      78
 
EcoRV GAT'ATC

Cuts at:      0      87    2652    2694    3693 
   Size:         87    2565      42     999

  Fragments arranged by size: 

                2565     999      87      42
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1534    2286    3693 
   Size:       1534     752    1407

  Fragments arranged by size: 

                1534    1407     752
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26    2681    2713    2806    2838    3154    3186    3408 
   Size:         26    2655      32      93      32     316      32     222

Cuts at:   3408    3426    3440    3458    3534    3566    3693 
   Size:         18      14      18      76      32     127

  Fragments arranged by size: 

                2655     316     222     127      93      76      32      32
                  32      32      26      18      18      14
 
HincII GTy'rAC

Cuts at:      0    1966    2066    2317    3203    3693 
   Size:       1966     100     251     886     490

  Fragments arranged by size: 

                1966     886     490     251     100
 
MfeI C'AATT_G

Cuts at:      0    3506    3693 
   Size:       3506     187
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     979    1649    3693 
   Size:        979     670    2044

  Fragments arranged by size: 

                2044     979     670
 
MslI CAynn'nnrTG

Cuts at:      0     538     718     943    1424    1826    2202    2911    2924 
   Size:        538     180     225     481     402     376     709      13

Cuts at:   2924    3373    3693 
   Size:        449     320

  Fragments arranged by size: 

                 709     538     481     449     402     376     320     225
                 180      13
 
MspA1I CmG'CkG

Cuts at:      0    1282    2644    3693 
   Size:       1282    1362    1049

  Fragments arranged by size: 

                1362    1282    1049
 
NcoI C'CATG_G

Cuts at:      0     485    3578    3693 
   Size:        485    3093     115

  Fragments arranged by size: 

                3093     485     115
 
NdeI CA'TA_TG

Cuts at:      0     413    3693 
   Size:        413    3280
 
NheI G'CTAG_C

Cuts at:      0    3144    3693 
   Size:       3144     549
 
NsiI A_TGCA'T

Cuts at:      0    1010    2052    3693 
   Size:       1010    1042    1641

  Fragments arranged by size: 

                1641    1042    1010
 
NspI r_CATG'y

Cuts at:      0    1429    1452    1895    2175    2427    3264    3693 
   Size:       1429      23     443     280     252     837     429

  Fragments arranged by size: 

                1429     837     443     429     280     252      23
 
PciI A'CATG_T

Cuts at:      0    2423    3693 
   Size:       2423    1270
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1522    3693 
   Size:       1522    2171
 
PsiI TTA'TAA

Cuts at:      0    1067    2678    3464    3693 
   Size:       1067    1611     786     229

  Fragments arranged by size: 

                1611    1067     786     229
 
PspOMI G'GGCC_C

Cuts at:      0     144    3693 
   Size:        144    3549
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     785     817    3693 
   Size:        785      32    2876

  Fragments arranged by size: 

                2876     785      32
 
PstI C_TGCA'G

Cuts at:      0    1221    2204    2649    3693 
   Size:       1221     983     445    1044

  Fragments arranged by size: 

                1221    1044     983     445
 
PvuII CAG'CTG

Cuts at:      0    1282    2644    3693 
   Size:       1282    1362    1049

  Fragments arranged by size: 

                1362    1282    1049
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1312    1505    3494    3693 
   Size:       1312     193    1989     199

  Fragments arranged by size: 

                1989    1312     199     193
 
ScaI AGT'ACT

Cuts at:      0    1780    3693 
   Size:       1780    1913
 
SfcI C'TryA_G

Cuts at:      0      40    1217    1736    2200    2645    3233    3693 
   Size:         40    1177     519     464     445     588     460

  Fragments arranged by size: 

                1177     588     519     464     460     445      40
 
SphI G_CATG'C

Cuts at:      0    1429    3264    3693 
   Size:       1429    1835     429

  Fragments arranged by size: 

                1835    1429     429
 
SspI AAT'ATT

Cuts at:      0     863    1854    3693 
   Size:        863     991    1839

  Fragments arranged by size: 

                1839     991     863
 
StuI AGG'CCT

Cuts at:      0    2359    3693 
   Size:       2359    1334
 
StyI C'CwwG_G

Cuts at:      0     485    3578    3693 
   Size:        485    3093     115

  Fragments arranged by size: 

                3093     485     115
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     341    3693 
   Size:        341    3352
 
TatI w'GTAC_w

Cuts at:      0     879    1161    1778    2206    3693 
   Size:        879     282     617     428    1487

  Fragments arranged by size: 

                1487     879     617     428     282
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1435    1467    1622    1654    3693 
   Size:       1435      32     155      32    2039

  Fragments arranged by size: 

                2039    1435     155      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1361    3693 
   Size:       1361    2332
 
XbaI T'CTAG_A

Cuts at:      0    1404    3693 
   Size:       1404    2289
 
XmnI GAAnn'nnTTC

Cuts at:      0     508    1208    2012    3671    3693 
   Size:        508     700     804    1659      22

  Fragments arranged by size: 

                1659     804     700     508      22
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI     AleI     AlfI     ApaI     ApoI
     AseI     BaeI    BanII     BbsI     BcgI    BciVI     BclI    BfrBI
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BsrDI    BsrFI    BsrGI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI     BtsI     DraI   DraIII     DrdI     EaeI
     EarI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    Hin4I   HincII
     MfeI     MmeI     MslI   MspA1I     NcoI     NdeI     NheI     NsiI
     NspI     PciI    PflMI     PsiI   PspOMI     PsrI     PstI    PvuII
     SapI     ScaI     SfcI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AloI
    AlwNI    ApaLI     AscI    AsiSI     AvaI    AvrII    BamHI     BanI
     BbeI    BbvCI     BglI    BglII    BmgBI    BpuEI     BsaI    BsaAI
    BsaHI    BsiEI    BsiWI    BsmBI    BspMI    BsrBI   BssHII    BssSI
   BstAPI   BstEII    BtgZI     ClaI    CspCI     EagI     EciI  EcoICRI
    EcoNI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MluI     MscI     NaeI     NarI   NgoMIV     NotI     NruI
     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SwaI     XcmI     XhoI     XmaI     ZraI
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