Restriction map for pF1KE1028
 (Circular) (Six-base) MAPSORT of: pF1KE1028.seq  Check: 5354  from: 1  to: 6764



pF1KE1028  6764 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    111     111 
   Size:       6764
 
AccI GT'mk_AC

Cuts at:   2422    3802    2422 
   Size:       1380    5384
 
Acc65I G'GTAC_C

Cuts at:   3780    3780 
   Size:       6764
 
AclI AA'CG_TT

Cuts at:   1080    6604    1080 
   Size:       5524    1240
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    159    1397    2512    2782    3210    4589    5021    5374    6036 
   Size:       1238    1115     270     428    1379     432     353     662

Cuts at:   6036     159 
   Size:        887

  Fragments arranged by size: 

                1379    1238    1115     887     662     432     428     353
                 270
 
AfeI AGC'GCT

Cuts at:    489    2815     489 
   Size:       2326    4438
 
AflIII A'CryG_T

Cuts at:   1980    4116    5916    6197    1980 
   Size:       2136    1800     281    2547

  Fragments arranged by size: 

                2547    2136    1800     281
 
AgeI A'CCGG_T

Cuts at:    368     368 
   Size:       6764
 
AleI CACnn'nnGTG

Cuts at:   1753    1753 
   Size:       6764
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1571    1571 
   Size:       6764
 
AlwNI CAG_nnn'CTG

Cuts at:   1104    2782    2985    3552    4069    5507    1104 
   Size:       1678     203     567     517    1438    2361

  Fragments arranged by size: 

                2361    1678    1438     567     517     203
 
ApaLI G'TGCA_C

Cuts at:   5602    5602 
   Size:       6764
 
ApoI r'AATT_y

Cuts at:    947    1156    2287    2933    3081    3661    3768    6117    6373 
   Size:        209    1131     646     148     580     107    2349     256

Cuts at:   6373     947 
   Size:       1338

  Fragments arranged by size: 

                2349    1338    1131     646     580     256     209     148
                 107
 
AseI AT'TA_AT

Cuts at:     20    1325    2228      20 
   Size:       1305     903    4556

  Fragments arranged by size: 

                4556    1305     903
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6764
 
AvaI C'yCGr_G

Cuts at:   1445    2585    3784    1445 
   Size:       1140    1199    4425

  Fragments arranged by size: 

                4425    1199    1140
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    590     623     590 
   Size:         33    6731
 
BamHI G'GATC_C

Cuts at:   3789    3789 
   Size:       6764
 
BanI G'GyrC_C

Cuts at:    415    3780    4194    4442    4477     415 
   Size:       3365     414     248      35    2702

  Fragments arranged by size: 

                3365    2702     414     248      35
 
BanII G_rGCy'C

Cuts at:    774    3778    4808     774 
   Size:       3004    1030    2730

  Fragments arranged by size: 

                3004    2730    1030
 
BbeI G_GCGC'C

Cuts at:   4198    4446    4198 
   Size:        248    6516
 
BbsI GAAGACnn'nnnn_

Cuts at:   1376    1376 
   Size:       6764
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    796     830     796 
   Size:         34    6730
 
BciVI GTATCCnnnnn_n'

Cuts at:   1890    4662    5718    6764    1890 
   Size:       2772    1056    1046    1890

  Fragments arranged by size: 

                2772    1890    1056    1046
 
BclI T'GATC_A

Cuts at:   1934    2241    3707    4284    1934 
   Size:        307    1466     577    4414

  Fragments arranged by size: 

                4414    1466     577     307
 
BglII A'GATC_T

Cuts at:   1572    4279    6316    1572 
   Size:       2707    2037    2020

  Fragments arranged by size: 

                2707    2037    2020
 
BlpI GC'TnA_GC

Cuts at:    839     884    3560    3876     839 
   Size:         45    2676     316    3727

  Fragments arranged by size: 

                3727    2676     316      45
 
Bme1580I G_kGCm'C

Cuts at:   4389    4482    5606    4389 
   Size:         93    1124    5547

  Fragments arranged by size: 

                5547    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   2111    2458    3976    4057    4392    6536    2111 
   Size:        347    1518      81     335    2144    2339

  Fragments arranged by size: 

                2339    2144    1518     347     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5131    5131 
   Size:       6764
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1420    1874    3410    1420 
   Size:        454    1536    4774

  Fragments arranged by size: 

                4774    1536     454
 
Bpu10I CC'TnA_GC

Cuts at:   2372    2372 
   Size:       6764
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1190    1288    3034    3293    3667    3940    4819    5533    5831 
   Size:         98    1746     259     374     273     879     714     298

Cuts at:   5831    1190 
   Size:       2123

  Fragments arranged by size: 

                2123    1746     879     714     374     298     273     259
                  98
 
BsaI GGTCTCn'nnnn_

Cuts at:   2266    6468    2266 
   Size:       4202    2562
 
BsaAI yAC'GTr

Cuts at:   4117    4117 
   Size:       6764
 
BsaBI GATnn'nnATC

Cuts at:      6    4278    6315       6 
   Size:       4272    2037     455

  Fragments arranged by size: 

                4272    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   4195    4443    5145    6641    4195 
   Size:        248     702    1496    4318

  Fragments arranged by size: 

                4318    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    368    3953    4474    5152    5563    5710    5984     368 
   Size:       3585     521     678     411     147     274    1148

  Fragments arranged by size: 

                3585    1148     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   2027    2276    4189    4947    5612    6020    2027 
   Size:        249    1913     758     665     408    2771

  Fragments arranged by size: 

                2771    1913     758     665     408     249
 
BsiEI CG_ry'CG

Cuts at:    104    4306    4352    5582     104 
   Size:       4202      46    1230    1286

  Fragments arranged by size: 

                4202    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   2097    3778    4556    4746    5606    2097 
   Size:       1681     778     190     860    3255

  Fragments arranged by size: 

                3255    1681     860     778     190
 
BsmI GAATG_Cn'

Cuts at:   1542    1542 
   Size:       6764
 
BsmBI CGTCTCn'nnnn_

Cuts at:    759     759 
   Size:       6764
 
Bsp1286I G_dGCh'C

Cuts at:    774    2097    3778    4389    4482    4556    4746    4808    5606 
   Size:       1323    1681     611      93      74     190      62     798

Cuts at:   5606     774 
   Size:       1932

  Fragments arranged by size: 

                1932    1681    1323     798     611     190      93      74
                  62
 
BspHI T'CATG_A

Cuts at:   2856    5196    2856 
   Size:       2340    4424
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    111    2213    3814    4028    4330    4711     111 
   Size:       2102    1601     214     302     381    2164

  Fragments arranged by size: 

                2164    2102    1601     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    5056    5110    6594    6759      50 
   Size:       5006      54    1484     165      55

  Fragments arranged by size: 

                5006    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   4676    4676 
   Size:       6764
 
BsrFI r'CCGG_y

Cuts at:    368    4762    4943     368 
   Size:       4394     181    2189

  Fragments arranged by size: 

                4394    2189     181
 
BsrGI T'GTAC_A

Cuts at:    110    3603     110 
   Size:       3493    3271
 
BssSI C'ACGA_G

Cuts at:    320     812    5035    5743     320 
   Size:        492    4223     708    1341

  Fragments arranged by size: 

                4223    1341     708     492
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1104    1558    2898    4141    1104 
   Size:        454    1340    1243    3727

  Fragments arranged by size: 

                3727    1340    1243     454
 
BstBI TT'CG_AA

Cuts at:    141     402    3006    5125     141 
   Size:        261    2604    2119    1780

  Fragments arranged by size: 

                2604    2119    1780     261
 
BstEII G'GTnAC_C

Cuts at:    371    2071     371 
   Size:       1700    5064
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3388    3388 
   Size:       6764
 
BstYI r'GATC_y

Cuts at:      1     168     211    1227    1425    1572    3789    4256    4279 
   Size:        167      43    1016     198     147    2217     467      23

Cuts at:   4279    4614    4860    5166    5178    5264    6316       1 
   Size:        335     246     306      12      86    1052     449

  Fragments arranged by size: 

                2217    1052    1016     467     449     335     306     246
                 198     167     147      86      43      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4714    4895    5058    4714 
   Size:        181     163    6420

  Fragments arranged by size: 

                6420     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1763    3025    3369    4007    1763 
   Size:       1262     344     638    4520

  Fragments arranged by size: 

                4520    1262     638     344
 
ClaI AT'CG_AT

Cuts at:    380    3145     380 
   Size:       2765    3999
 
DraI TTT'AAA

Cuts at:   1625    2292    3763    1625 
   Size:        667    1471    4626

  Fragments arranged by size: 

                4626    1471     667
 
DraIII CAC_nnn'GTG

Cuts at:   3757    3757 
   Size:       6764
 
DrdI GACnn_nn'nnGTC

Cuts at:   4470    5814    4470 
   Size:       1344    5420
 
EaeI y'GGCC_r

Cuts at:   3722    4349    4523    4914    4941    6286    3722 
   Size:        627     174     391      27    1345    4200

  Fragments arranged by size: 

                4200    1345     627     391     174      27
 
EagI C'GGCC_G

Cuts at:   4349    4349 
   Size:       6764
 
EarI CTCTTCn'nnn_

Cuts at:    399     692    1526    2335    2485    2797    2912    3116    4787 
   Size:        293     834     809     150     312     115     204    1671

Cuts at:   4787    4997     399 
   Size:        210    2166

  Fragments arranged by size: 

                2166    1671     834     809     312     293     210     204
                 150     115
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4972    5714    5860    6574    4972 
   Size:        742     146     714    5162

  Fragments arranged by size: 

                5162     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   3776    3776 
   Size:       6764
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    159    1397    1420    1874    2512    2782    3210    3410    4589 
   Size:       1238      23     454     638     270     428     200    1179

Cuts at:   4589    5021    5374    6036     159 
   Size:        432     353     662     887

  Fragments arranged by size: 

                1238    1179     887     662     638     454     432     428
                 353     270     200      23
 
EcoO109I rG'GnC_Cy

Cuts at:   3903    3903 
   Size:       6764
 
EcoRI G'AATT_C

Cuts at:   3768    3768 
   Size:       6764
 
EcoRV GAT'ATC

Cuts at:    915     915 
   Size:       6764
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    415     527     994    4783     415 
   Size:        112     467    3789    2396

  Fragments arranged by size: 

                3789    2396     467     112
 
FspI TGC'GCA

Cuts at:   2209    4545    2209 
   Size:       2336    4428
 
HaeII r_GCGC'y

Cuts at:    491    2817    4198    4446    5676    6447     491 
   Size:       2326    1381     248    1230     771     808

  Fragments arranged by size: 

                2326    1381    1230     808     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1938    1970    2871    2903    4606    4636    4638    4668    1938 
   Size:         32     901      32    1703      30       2      30    4034

  Fragments arranged by size: 

                4034    1703     901      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   2524    3054    3200    3378    3803    2524 
   Size:        530     146     178     425    5485

  Fragments arranged by size: 

                5485     530     425     178     146
 
HindIII A'AGCT_T

Cuts at:   1970    2403    1970 
   Size:        433    6331
 
HpaI GTT'AAC

Cuts at:   3054    3200    3054 
   Size:        146    6618
 
KasI G'GCGC_C

Cuts at:   4194    4442    4194 
   Size:        248    6516
 
KpnI G_GTAC'C

Cuts at:   3784    3784 
   Size:       6764
 
MfeI C'AATT_G

Cuts at:    161     161 
   Size:       6764
 
MluI A'CGCG_T

Cuts at:   6197    6197 
   Size:       6764
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1001    3375    5523    5707    1001 
   Size:       2374    2148     184    2058

  Fragments arranged by size: 

                2374    2148    2058     184
 
MscI TGG'CCA

Cuts at:   3724    4525    3724 
   Size:        801    5963
 
MslI CAynn'nnrTG

Cuts at:   1753    2090    2843    3701    4753    4880    1753 
   Size:        337     753     858    1052     127    3637

  Fragments arranged by size: 

                3637    1052     858     753     337     127
 
MspA1I CmG'CkG

Cuts at:    288    2031    3655    3875    4189    4549    5333    5578     288 
   Size:       1743    1624     220     314     360     784     245    1474

  Fragments arranged by size: 

                1743    1624    1474     784     360     314     245     220
            
 
NaeI GCC'GGC

Cuts at:   4945    4945 
   Size:       6764
 
NarI GG'CG_CC

Cuts at:   4195    4443    4195 
   Size:        248    6516
 
NdeI CA'TA_TG

Cuts at:   2840    2840 
   Size:       6764
 
NgoMIV G'CCGG_C

Cuts at:   4943    4943 
   Size:       6764
 
NspI r_CATG'y

Cuts at:   1690    1754    1984    2413    2901    3610    3817    4317    5920 
   Size:         64     230     429     488     709     207     500    1603

Cuts at:   5920    1690 
   Size:       2534

  Fragments arranged by size: 

                2534    1603     709     500     488     429     230     207
                  64
 
PciI A'CATG_T

Cuts at:   1980    5916    1980 
   Size:       3936    2828
 
PflMI CCAn_nnn'nTGG

Cuts at:   3552    3552 
   Size:       6764
 
PfoI T'CCnGG_A

Cuts at:   1851    1851 
   Size:       6764
 
PmeI GTTT'AAAC

Cuts at:   3763    3763 
   Size:       6764
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5210    5242    5210 
   Size:         32    6732
 
PspXI vC'TCGA_Gb

Cuts at:   1445    2585    1445 
   Size:       1140    5624
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   3587    3619    3587 
   Size:         32    6732
 
PstI C_TGCA'G

Cuts at:   3811    4496    3811 
   Size:        685    6079
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6764
 
PvuII CAG'CTG

Cuts at:   2031    3655    4189    4549    2031 
   Size:       1624     534     360    4246

  Fragments arranged by size: 

                4246    1624     534     360
 
RsrII CG'GwC_CG

Cuts at:   4959    4959 
   Size:       6764
 
SacI G_AGCT'C

Cuts at:   3778    3778 
   Size:       6764
 
SalI G'TCGA_C

Cuts at:   3801    3801 
   Size:       6764
 
SapI GCTCTTCn'nnn_

Cuts at:   4787    4997    4787 
   Size:        210    6554
 
SbfI CC_TGCA'GG

Cuts at:   3811    3811 
   Size:       6764
 
ScaI AGT'ACT

Cuts at:   6281    6281 
   Size:       6764
 
SexAI A'CCwGG_T

Cuts at:   3252    3252 
   Size:       6764
 
SfcI C'TryA_G

Cuts at:     33    3807    4492    5460    5651      33 
   Size:       3774     685     968     191    1146

  Fragments arranged by size: 

                3774    1146     968     685     191
 
SfoI GGC'GCC

Cuts at:   4196    4444    4196 
   Size:        248    6516
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6764
 
SmaI CCC'GGG

Cuts at:   3786    3786 
   Size:       6764
 
SmlI C'TyrA_G

Cuts at:   1205    1303    1445    2585    3013    3308    3646    3919    4834 
   Size:         98     142    1140     428     295     338     273     915

Cuts at:   4834    5548    5810    1205 
   Size:        714     262    2159

  Fragments arranged by size: 

                2159    1140     915     714     428     338     295     273
                 262     142      98
 
SpeI A'CTAG_T

Cuts at:     67     850      67 
   Size:        783    5981
 
SphI G_CATG'C

Cuts at:   2413    2901    3817    4317    2413 
   Size:        488     916     500    4860

  Fragments arranged by size: 

                4860     916     500     488
 
SspI AAT'ATT

Cuts at:   1471    2217    1471 
   Size:        746    6018
 
StuI AGG'CCT

Cuts at:   3636    3636 
   Size:       6764
 
StyI C'CwwG_G

Cuts at:   1034    3470    3898    4252    1034 
   Size:       2436     428     354    3546

  Fragments arranged by size: 

                3546    2436     428     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   4347    4480    4939    5148    4347 
   Size:        133     459     209    5963

  Fragments arranged by size: 

                5963     459     209     133
 
TatI w'GTAC_w

Cuts at:    110    3041    3165    3603    6279     110 
   Size:       2931     124     438    2676     595

  Fragments arranged by size: 

                2931    2676     595     438     124
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3389    3421    6281    6313    3389 
   Size:         32    2860      32    3840

  Fragments arranged by size: 

                3840    2860      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2077    4561    2077 
   Size:       2484    4280
 
XbaI T'CTAG_A

Cuts at:    714    2299    3795     714 
   Size:       1585    1496    3683

  Fragments arranged by size: 

                3683    1585    1496
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   4259    4259 
   Size:       6764
 
XhoI C'TCGA_G

Cuts at:   1445    2585    1445 
   Size:       1140    5624
 
XmaI C'CCGG_G

Cuts at:   3784    3784 
   Size:       6764
 
XmnI GAAnn'nnTTC

Cuts at:   2362    3191    2362 
   Size:        829    5935
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
     BaeI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI     PpiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     AloI     ApaI     AscI    AvrII    BbvCI
    BfrBI     BglI    BmgBI     BmtI    BsaXI    BseRI     BsgI    BsiWI
    BspEI   BssHII  BstZ17I   Bsu36I     BtgI    CspCI    EcoNI     FseI
    FspAI     NcoI     NheI     NotI     NruI     NsiI     PacI     PasI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    SacII    SanDI     SfiI
    SgrAI    SnaBI     SrfI     SwaI     ZraI
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