Restriction map for pF1KB9480
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# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AvrII       BamHI       BbrPI       BciVI       
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsiWI       BsmI        
BspDI       BspLU11I    BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
Eco47III    Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MluI        MseI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacII       SalI        
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SspI        SwaI        Tru9I       
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccIII        185  TCCGGA               T^CCGGA
AcsI         1642  AAATTC               [GA]^AATT[CT]
AflIII       1139  ACGTGT               A^C[GA][CT]GT
AgeI          311  ACCGGT               A^CCGGT
AhdI         2449  GACTTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        445  GTGCAC               G^TGCAC
ApaLI         445  GTGCAC               G^TGCAC
ApoI         1642  AAATTC               [GA]^AATT[CT]
Asp700I      1128  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2449  GACTTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1221  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         2031  CTCGGG               C^[CT]CG[GA]G
BbeI         1696  GGCGCC               GGCGC^C
BcgI          800  GCACAGTTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          929  TGATCA               T^GATCA
BcnI          420  CCGGG                CC^[GC]GG
BfaI         2116  CTAG                 C^TAG
BglI         1935  GCCCTTCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI         185  TCCGGA               T^CCGGA
BsiEI        1808  CGGCCG               CG[GA][CT]^CG
BsmBI         322  CGTCTC               CGTCTC(1/5)
BsoBI        2031  CTCGGG               C^[CT]CG[GA]G
BspEI         185  TCCGGA               T^CCGGA
BspHI        1901  TCATGA               T^CATGA
BsrBI         874  GAGCGG               CCGCTC(-3/-3)
BssHII       1801  GCGCGC               G^CGCGC
BstAPI         99  GCAGGGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI         1808  CGGCCG               C^GGCCG
Eam1105I     2449  GACTTCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1808  CGGCCG               C^GGCCG
Eco52I       1808  CGGCCG               C^GGCCG
EcoNI         183  CCTCCGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          929  TGATCA               T^GATCA
KasI         1696  GGCGCC               G^GCGCC
MaeI         2116  CTAG                 C^TAG
MroI          185  TCCGGA               T^CCGGA
NaeI         2004  GCCGGC               GCC^GGC
NarI         1696  GGCGCC               GG^CGCC
NciI          420  CCGGG                CC^[GC]GG
NgoMIV       2004  GCCGGC               G^CCGGC
PflFI        1221  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2046  CCAGGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         311  ACCGGT               A^CCGGT
PpuMI         539  GGGACCT              [GA]G^G[AT]CC[CT]
RcaI         1901  TCATGA               T^CATGA
SacI         2370  GAGCTC               GAGCT^C
SapI         2085  GCTCTTC              GCTCTTC(1/4)
SbfI           96  CCTGCAGG             CCTGCA^GG
ScaI         1001  AGTACT               AGT^ACT
SfoI         1696  GGCGCC               GGC^GCC
SphI          665  GCATGC               GCATG^C
Sse8387I       96  CCTGCAGG             CCTGCA^GG
Tsp509I      1643  AATT                 ^AATT
Tth111I      1221  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2046  CCAGGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1128  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccII        1802  CGCG                 CG^CG
             2058  CGCG                
AviII        1757  TGCGCA               TGC^GCA
             2102  TGCGCA              
BalI           17  TGGCCA               TGG^CCA
              948  TGGCCA              
BanI           28  GGCACC               G^G[CT][GA]CC
             1696  GGCGCC              
BbsI         1033  GAAGAC               GAAGAC(2/6)
             1888  GTCTTC              
BbvCI        1724  CCTCAGC              CCTCAGC(-5/-2)
             1651  GCTGAGG             
BpuAI        1033  GAAGAC               GAAGAC(2/6)
             1888  GTCTTC              
BsaWI         185  TCCGGA               [AT]^CCGG[AT]
              311  ACCGGT              
BseRI        1330  CTCCTC               GAGGAG(10/8)
             2410  GAGGAG              
Bsp1407I     1778  TGTACA               T^GTACA
             1949  TGTACA              
BsrFI         311  ACCGGT               [GA]^CCGG[CT]
             2004  GCCGGC              
BsrGI        1778  TGTACA               T^GTACA
             1949  TGTACA              
BstUI        1802  CGCG                 CG^CG
             2058  CGCG                
BstXI         125  CCACCGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1391  CCAAGCCTGTGG        
BstYI         416  AGATCC               [GA]^GATC[CT]
             2024  AGATCC              
Cfr10I        311  ACCGGT               [GA]^CCGG[CT]
             2004  GCCGGC              
DraII         101  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              539  GGGACCT             
EcoO109I      101  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              539  GGGACCT             
FspI         1757  TGCGCA               TGC^GCA
             2102  TGCGCA              
HaeII          73  AGCGCC               [GA]GCGC^[CT]
             1696  GGCGCC              
HincII        346  GTTGAC               GT[CT]^[GA]AC
              526  GTCAAC              
HindII        346  GTTGAC               GT[CT]^[GA]AC
              526  GTCAAC              
MflI          416  AGATCC               [GA]^GATC[CT]
             2024  AGATCC              
MluNI          17  TGGCCA               TGG^CCA
              948  TGGCCA              
MscI           17  TGGCCA               TGG^CCA
              948  TGGCCA              
MspA1I        463  CAGCGG               C[AC]G^C[GT]G
             1623  CCGCTG              
MvnI         1802  CGCG                 CG^CG
             2058  CGCG                
NcoI         1370  CCATGG               C^CATGG
             1745  CCATGG              
NspI          665  GCATGC               [GA]CATG^[CT]
             1952  ACATGC              
SexAI        1967  ACCTGGT              A^CC[AT]GGT
             2045  ACCAGGT             
SspBI        1778  TGTACA               T^GTACA
             1949  TGTACA              
StuI         1767  AGGCCT               AGG^CCT
             1841  AGGCCT              
TfiI          737  GAATC                G^A[AT]TC
             2487  GATTC               
XcmI          357  CCAAAGGGACAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2178  CCACGTCTGCTTTGG     
XhoII         416  AGATCC               [GA]^GATC[CT]
             2024  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccI        AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaII       BanII       
BbvI        BmyI        BpmI        BsaHI       BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EarI        EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     
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