Restriction map for pF1KB5451
 (Linear) (Six-base) MAPSORT of: KIBB5451.seq  Check: 844  from: 1  to: 1116



KIBB5451.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      46    1116 
   Size:         46    1070
 
AatII G_ACGT'C

Cuts at:      0     469    1116 
   Size:        469     647
 
AccI GT'mk_AC

Cuts at:      0     140    1116 
   Size:        140     976
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     545    1057    1116 
   Size:        115     430     512      59

  Fragments arranged by size: 

                 512     430     115      59
 
AflIII A'CryG_T

Cuts at:      0    1043    1116 
   Size:       1043      73
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1102    1116 
   Size:       1102      14
 
AlwNI CAG_nnn'CTG

Cuts at:      0     588     956    1116 
   Size:        588     368     160

  Fragments arranged by size: 

                 588     368     160
 
ApoI r'AATT_y

Cuts at:      0     176     323     513     811    1116 
   Size:        176     147     190     298     305

  Fragments arranged by size: 

                 305     298     190     176     147
 
AvaI C'yCGr_G

Cuts at:      0     231    1020    1116 
   Size:        231     789      96

  Fragments arranged by size: 

                 789     231      96
 
BanI G'GyrC_C

Cuts at:      0     130    1116 
   Size:        130     986
 
BanII G_rGCy'C

Cuts at:      0     272     847    1116 
   Size:        272     575     269

  Fragments arranged by size: 

                 575     272     269
 
BbeI G_GCGC'C

Cuts at:      0     134    1116 
   Size:        134     982
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     551    1116 
   Size:        551     565
 
BglI GCCn_nnn'nGGC

Cuts at:      0     620    1116 
   Size:        620     496
 
BmgBI CAC'GTC

Cuts at:      0     419    1116 
   Size:        419     697
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     868    1116 
   Size:        868     248
 
BsaAI yAC'GTr

Cuts at:      0    1086    1116 
   Size:       1086      30
 
BsaHI Gr'CG_yC

Cuts at:      0     131     311     466    1109    1116 
   Size:        131     180     155     643       7

  Fragments arranged by size: 

                 643     180     155     131       7
 
BsaWI w'CCGG_w

Cuts at:      0     116    1116 
   Size:        116    1000
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     280    1116 
   Size:        280     836
 
BseYI C'CCAG_C

Cuts at:      0     706     943    1116 
   Size:        706     237     173

  Fragments arranged by size: 

                 706     237     173
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1108    1116 
   Size:       1108       8
 
BsiHKAI G_wGCw'C

Cuts at:      0     272    1116 
   Size:        272     844
 
Bsp1286I G_dGCh'C

Cuts at:      0     272     847    1116 
   Size:        272     575     269

  Fragments arranged by size: 

                 575     272     269
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      46    1116 
   Size:         46    1070
 
BsrDI GCAATG_nn'

Cuts at:      0     201    1116 
   Size:        201     915
 
BsrFI r'CCGG_y

Cuts at:      0     190    1116 
   Size:        190     926
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     956    1116 
   Size:        956     160
 
BstEII G'GTnAC_C

Cuts at:      0     984    1116 
   Size:        984     132
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     633     796    1116 
   Size:        633     163     320

  Fragments arranged by size: 

                 633     320     163
 
BtgI C'CryG_G

Cuts at:      0     460    1116 
   Size:        460     656
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     282    1116 
   Size:        282     834
 
EaeI y'GGCC_r

Cuts at:      0     188     341    1097    1116 
   Size:        188     153     756      19

  Fragments arranged by size: 

                 756     188     153      19
 
EarI CTCTTCn'nnn_

Cuts at:      0     866    1063    1116 
   Size:        866     197      53

  Fragments arranged by size: 

                 866     197      53
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     916    1116 
   Size:        916     200
 
EcoICRI GAG'CTC

Cuts at:      0     270    1116 
   Size:        270     846
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     545    1057    1116 
   Size:        115     430     512      59

  Fragments arranged by size: 

                 512     430     115      59
 
EcoO109I rG'GnC_Cy

Cuts at:      0     757    1116 
   Size:        757     359
 
EcoRI G'AATT_C

Cuts at:      0     323    1116 
   Size:        323     793
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     609     883    1116 
   Size:        609     274     233

  Fragments arranged by size: 

                 609     274     233
 
HaeII r_GCGC'y

Cuts at:      0     134    1116 
   Size:        134     982
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      75     107     281     313    1116 
   Size:         75      32     174      32     803

  Fragments arranged by size: 

                 803     174      75      32      32
 
KasI G'GCGC_C

Cuts at:      0     130    1116 
   Size:        130     986
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     872    1116 
   Size:        350     522     244

  Fragments arranged by size: 

                 522     350     244
 
MscI TGG'CCA

Cuts at:      0     343    1099    1116 
   Size:        343     756      17

  Fragments arranged by size: 

                 756     343      17
 
MslI CAynn'nnrTG

Cuts at:      0     631    1013    1084    1116 
   Size:        631     382      71      32

  Fragments arranged by size: 

                 631     382      71      32
 
MspA1I CmG'CkG

Cuts at:      0     172     279     898     947    1116 
   Size:        172     107     619      49     169

  Fragments arranged by size: 

                 619     172     169     107      49
 
NaeI GCC'GGC

Cuts at:      0     192    1116 
   Size:        192     924
 
NarI GG'CG_CC

Cuts at:      0     131    1116 
   Size:        131     985
 
NgoMIV G'CCGG_C

Cuts at:      0     190    1116 
   Size:        190     926
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     541    1116 
   Size:        541     575
 
PspXI vC'TCGA_Gb

Cuts at:      0    1020    1116 
   Size:       1020      96
 
PvuII CAG'CTG

Cuts at:      0     279    1116 
   Size:        279     837
 
SacI G_AGCT'C

Cuts at:      0     272    1116 
   Size:        272     844
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1063    1116 
   Size:       1063      53
 
SfoI GGC'GCC

Cuts at:      0     132    1116 
   Size:        132     984
 
SmlI C'TyrA_G

Cuts at:      0    1020    1116 
   Size:       1020      96
 
StyI C'CwwG_G

Cuts at:      0     446     695     753    1116 
   Size:        446     249      58     363

  Fragments arranged by size: 

                 446     363     249      58
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     665    1116 
   Size:        665     451
 
TatI w'GTAC_w

Cuts at:      0    1046    1116 
   Size:       1046      70
 
XbaI T'CTAG_A

Cuts at:      0     180    1116 
   Size:        180     936
 
XhoI C'TCGA_G

Cuts at:      0    1020    1116 
   Size:       1020      96
 
ZraI GAC'GTC

Cuts at:      0     467    1116 
   Size:        467     649
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AlfI    AlwNI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI     BglI    BmgBI     BsaI
    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrDI    BsrFI   BstAPI   BstEII    BstXI     BtgI    BtgZI
     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV    PflMI    PspXI    PvuII     SacI     SapI     SfoI
     SmlI     StyI    TaqII     TatI     XbaI     XhoI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI
   BssHII    BssSI    BstBI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI    EcoNI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XcmI     XmaI     XmnI
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