Restriction map for pF1KB9659
 (Linear) (Six-base) MAPSORT of: KIBB9659.seq  Check: 3733  from: 1  to: 1464



KIBB9659.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146    1464 
   Size:        146    1318
 
AgeI A'CCGG_T

Cuts at:      0     757    1464 
   Size:        757     707
 
AleI CACnn'nnGTG

Cuts at:      0     506     590     926    1262    1464 
   Size:        506      84     336     336     202

  Fragments arranged by size: 

                 506     336     336     202      84
 
ApoI r'AATT_y

Cuts at:      0     161     245     329     413     581     749     833     917 
   Size:        161      84      84      84     168     168      84      84

Cuts at:    917    1085    1337    1464 
   Size:        168     252     127

  Fragments arranged by size: 

                 252     168     168     168     161     127      84      84
                  84      84      84
 
BanI G'GyrC_C

Cuts at:      0    1244    1464 
   Size:       1244     220
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     134    1051    1464 
   Size:        134     917     413

  Fragments arranged by size: 

                 917     413     134
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     157    1464 
   Size:        157    1307
 
BfrBI ATG'CAT

Cuts at:      0    1005    1464 
   Size:       1005     459
 
BglII A'GATC_T

Cuts at:      0     897    1464 
   Size:        897     567
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1031    1464 
   Size:       1031     433
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1299    1464 
   Size:       1299     165
 
BsaWI w'CCGG_w

Cuts at:      0     757    1464 
   Size:        757     707
 
BsmI GAATG_Cn'

Cuts at:      0     277     697    1007    1464 
   Size:        277     420     310     457

  Fragments arranged by size: 

                 457     420     310     277
 
BsrFI r'CCGG_y

Cuts at:      0     757    1464 
   Size:        757     707
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     313    1464 
   Size:        313    1151
 
BstYI r'GATC_y

Cuts at:      0      85     897    1464 
   Size:         85     812     567

  Fragments arranged by size: 

                 812     567      85
 
BtsI GCAGTG_nn'

Cuts at:      0     642     726    1464 
   Size:        642      84     738

  Fragments arranged by size: 

                 738     642      84
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     264     299     684     719    1464 
   Size:        264      35     385      35     745

  Fragments arranged by size: 

                 745     385     264      35      35
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     678    1464 
   Size:        678     786
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146    1031    1464 
   Size:        146     885     433

  Fragments arranged by size: 

                 885     433     146
 
EcoRI G'AATT_C

Cuts at:      0     161     245     329     413     581     749     833     917 
   Size:        161      84      84      84     168     168      84      84

Cuts at:    917    1085    1337    1464 
   Size:        168     252     127

  Fragments arranged by size: 

                 252     168     168     168     161     127      84      84
                  84      84      84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     105     137     479     511    1464 
   Size:        105      32     342      32     953

  Fragments arranged by size: 

                 953     342     105      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1182    1464 
   Size:       1182     282
 
MslI CAynn'nnrTG

Cuts at:      0     104     170     254     338     422     506     590     674 
   Size:        104      66      84      84      84      84      84      84

Cuts at:    674     758     774     842     926    1094    1178    1262    1346 
   Size:         84      16      68      84     168      84      84      84

Cuts at:   1346    1464 
   Size:        118

  Fragments arranged by size: 

                 168     118     104      84      84      84      84      84
                  84      84      84      84      84      84      68      66
                  16
 
NsiI A_TGCA'T

Cuts at:      0    1007    1464 
   Size:       1007     457
 
NspI r_CATG'y

Cuts at:      0     135     704    1464 
   Size:        135     569     760

  Fragments arranged by size: 

                 760     569     135
 
SmlI C'TyrA_G

Cuts at:      0    1314    1464 
   Size:       1314     150
 
StuI AGG'CCT

Cuts at:      0     292     376     479     796     880    1132    1464 
   Size:        292      84     103     317      84     252     332

  Fragments arranged by size: 

                 332     317     292     252     103      84      84
 
XmnI GAAnn'nnTTC

Cuts at:      0     975    1464 
   Size:        975     489
 

 Enzymes that do cut:

     AcuI     AgeI     AleI     ApoI     BanI     BbsI    BciVI    BfrBI
    BglII     BpmI    BpuEI    BsaWI     BsmI    BsrFI   BstAPI    BstYI
     BtsI    CspCI     EciI  Eco57MI    EcoRI    Hin4I     MmeI     MslI
     NsiI     NspI     SmlI     StuI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI     BclI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI     DraI
   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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