Restriction map for pF1KB9642
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BbeI        BbrPI       BciVI       BclI        BfrI        
BglII       BlnI        BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstUI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco52I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MroI        MseI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

Aor51HI       563  AGCGCT               AGC^GCT
ApaI          665  GGGCCC               GGGCC^C
BamHI        1559  GGATCC               G^GATCC
BbsI          379  GTCTTC               GAAGAC(2/6)
BcgI          345  GCAGGGCAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          885  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      885  GCTCAGC              GC^T[ACGT]AGC
BpuAI         379  GTCTTC               GAAGAC(2/6)
BsaI         1316  GAGACC               GGTCTC(1/5)
BsmBI         604  CGTCTC               CGTCTC(1/5)
BsmI          233  GAATGC               GAATGC(1/-1)
BspMI        1225  ACCTGC               ACCTGC(4/8)
BstAPI        228  GCAGGGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1598  CCATGGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         885  GCTCAGC              GC^T[ACGT]AGC
CfoI          564  GCGC                 GCG^C
DrdI         1042  GACAAACTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      563  AGCGCT               AGC^GCT
HaeII         563  AGCGCT               [GA]GCGC^[CT]
HhaI          564  GCGC                 GCG^C
HinP1I        564  GCGC                 G^CGC
NheI          534  GCTAGC               G^CTAGC
PstI          343  CTGCAG               CTGCA^G
SexAI         500  ACCTGGT              A^CC[AT]GGT
SfaNI           4  GATGC                GCATC(5/9)
SfcI          343  CTGCAG               C^T[GA][CT]AG
SfiI           32  GGCCCCCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI          615  ACTAGT               A^CTAGT
TaqI         1260  TCGA                 T^CGA
Tsp509I       263  AATT                 ^AATT
TthHB8I      1260  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          160  GTAC                 GT^AC
              816  GTAC                
AlwNI         475  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              581  CAGTGCCTG           
BanI          798  GGTGCC               G^G[CT][GA]CC
             1719  GGCACC              
BglI           33  GCCCCCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1209  GCCTCCAGGGC         
BsgI          108  CTGCAC               GTGCAG(16/14)
             1420  GTGCAG              
BstEII       1050  GGTCACC              G^GT[ACGT]ACC
             1753  GGTAACC             
BstPI        1050  GGTCACC              G^GT[ACGT]ACC
             1753  GGTAACC             
EcoNI         252  CCTGCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1337  CCTGGATGAGG         
EcoO65I      1050  GGTCACC              G^GT[ACGT]ACC
             1753  GGTAACC             
MspA1I         87  CAGCTG               C[AC]G^C[GT]G
              659  CAGCTG              
PleI          170  GACTC                GAGTC(4/5)
             1477  GACTC               
PvuII          87  CAGCTG               CAG^CTG
              659  CAGCTG              
RsaI          160  GTAC                 GT^AC
              816  GTAC                
SacI          187  GAGCTC               GAGCT^C
              792  GAGCTC              
SapI         1179  GAAGAGC              GCTCTTC(1/4)
             1773  GCTCTTC             
SphI          928  GCATGC               GCATG^C
             1287  GCATGC              
StuI          387  AGGCCT               AGG^CCT
             1345  AGGCCT              
TfiI          362  GAATC                G^A[AT]TC
              855  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AluI        AlwI        AocI        AspHI       
AvaII       BalI        BanII       BbvCI       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrBI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Bsu36I      
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PpuMI       Sau3AI      Sau96I      ScrFI       
SmlI        StyI        TseI        Tsp45I      TspRI       
Van91I      XcmI        XhoII       
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