Restriction map for pF1KB9611
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AflIII      AgeI        AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AviII       
AvrII       BbrPI       BcgI        BciVI       BclI        
BfrI        BlnI        BsaAI       BsaWI       BseAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoRV       EcoT22I     FbaI        
FseI        FspI        HpaI        KpnI        KspI        
MfeI        MluI        MroI        MseI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    RcaI        
RsrII       SacII       SalI        SapI        ScaI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        SspBI       SspI        SwaI        
TfiI        Tru9I       XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII         139  GACGTC               GACGT^C
AcsI         1504  GAATTC               [GA]^AATT[CT]
AhdI         1369  GACATGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        520  GTGCAC               G^TGCAC
ApaLI         520  GTGCAC               G^TGCAC
ApoI         1504  GAATTC               [GA]^AATT[CT]
AspEI        1369  GACATGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          253  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1137  TGGCCA               TGG^CCA
BamHI         824  GGATCC               G^GATCC
BanI         1113  GGCGCC               G^G[CT][GA]CC
BbeI         1113  GGCGCC               GGCGC^C
BbvCI        1004  CCTCAGC              CCTCAGC(-5/-2)
BfaI          200  CTAG                 C^TAG
BsaBI        1479  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          277  CTGCAC               GTGCAG(16/14)
BsrBI         619  GAGCGG               CCGCTC(-3/-3)
BsrDI        1304  GCAATG               GCAATG(2/0)
BsrFI         485  GCCGGC               [GA]^CCGG[CT]
BstEII        846  GGTCACC              G^GT[ACGT]ACC
BstPI         846  GGTCACC              G^GT[ACGT]ACC
BstXI         440  CCAACTGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        485  GCCGGC               [GA]^CCGG[CT]
DraIII        388  CACGATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI           21  GACCCGCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1369  GACATGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           64  CTCTTC               CTCTTC(1/4)
EcoO65I       846  GGTCACC              G^GT[ACGT]ACC
EcoRI        1504  GAATTC               G^AATTC
HincII        259  GTCAAC               GT[CT]^[GA]AC
HindII        259  GTCAAC               GT[CT]^[GA]AC
HindIII       787  AAGCTT               A^AGCTT
KasI         1113  GGCGCC               G^GCGCC
Ksp632I        64  CTCTTC               CTCTTC(1/4)
MaeI          200  CTAG                 C^TAG
MamI         1479  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1137  TGGCCA               TGG^CCA
MscI         1137  TGGCCA               TGG^CCA
MslI         1704  CACCACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          485  GCCGGC               GCC^GGC
NarI         1113  GGCGCC               GG^CGCC
NgoMIV        485  GCCGGC               G^CCGGC
PaeR7I        591  CTCGAG               C^TCGAG
PflFI         253  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1642  GGGACCC              [GA]G^G[AT]CC[CT]
PvuI         1010  CGATCG               CGAT^CG
PvuII        1148  CAGCTG               CAG^CTG
SacI          691  GAGCTC               GAGCT^C
SbfI          735  CCTGCAGG             CCTGCA^GG
SexAI         932  ACCAGGT              A^CC[AT]GGT
SfoI         1113  GGCGCC               GGC^GCC
SmlI          591  CTCGAG               C^T[CT][GA]AG
Sse8387I      735  CCTGCAGG             CCTGCA^GG
StuI           74  AGGCCT               AGG^CCT
Tsp509I      1505  AATT                 ^AATT
Tth111I       253  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          785  CCAAGCTTATCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          591  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AlwI          825  GATCC                GGATC(4/5)
              824  GGATC               
Asp700I       426  GAACCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              990  GAAGGAGTTC          
AspHI         520  GTGCAC               G[AT]GC[AT]^C
              691  GAGCTC              
AvaI          591  CTCGAG               C^[CT]CG[GA]G
              688  CCCGAG              
BanII         691  GAGCTC               G[GA]GC[CT]^C
             1044  GGGCTC              
BbsI          628  GTCTTC               GAAGAC(2/6)
             1204  GTCTTC              
BglI          184  GCCGTGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1513  GCCGCCTCGGC         
BglII         926  AGATCT               A^GATCT
             1478  AGATCT              
BlpI         1145  GCTCAGC              GC^T[ACGT]AGC
             1150  GCTGAGC             
Bpu1102I     1145  GCTCAGC              GC^T[ACGT]AGC
             1150  GCTGAGC             
BpuAI         628  GTCTTC               GAAGAC(2/6)
             1204  GTCTTC              
BsiEI         159  CGACCG               CG[GA][CT]^CG
             1010  CGATCG              
BsiHKAI       520  GTGCAC               G[AT]GC[AT]^C
              691  GAGCTC              
BsmBI         747  CGTCTC               CGTCTC(1/5)
              529  GAGACG              
BsoBI         591  CTCGAG               C^[CT]CG[GA]G
              688  CCCGAG              
BstAPI        119  GCAAGCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1583  GCATGTCCTGC         
CelII        1145  GCTCAGC              GC^T[ACGT]AGC
             1150  GCTGAGC             
EcoNI         561  CCTGTCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              735  CCTGCAGGAGG         
HaeII        1000  AGCGCC               [GA]GCGC^[CT]
             1113  GGCGCC              
HinfI         352  GAGTC                G^A[ACGT]TC
             1659  GACTC               
MaeII          56  ACGT                 A^CGT
              140  ACGT                
MspA1I       1148  CAGCTG               C[AC]G^C[GT]G
             1457  CCGCTG              
NcoI          228  CCATGG               C^CATGG
             1140  CCATGG              
PleI         1659  GACTC                GAGTC(4/5)
              352  GAGTC               
TaqI          592  TCGA                 T^CGA
              632  TCGA                
TthHB8I       592  TCGA                 T^CGA
              632  TCGA                
XmnI          426  GAACCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              990  GAAGGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AvaII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HpaII       HphI        ItaI        
MaeIII      MboI        MboII       MflI        MnlI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TseI        Tsp45I      
TspRI       Van91I      XhoII       
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