Restriction map for pF1KB9611
 (Linear) (Six-base) MAPSORT of: KIBB9611.seq  Check: 2997  from: 1  to: 1767



KIBB9611.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1766    1767 
   Size:       1766       1
 
AatII G_ACGT'C

Cuts at:      0     143    1767 
   Size:        143    1624
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1351    1667    1767 
   Size:       1351     316     100

  Fragments arranged by size: 

                1351     316     100
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1374    1767 
   Size:       1374     393
 
AleI CACnn'nnGTG

Cuts at:      0    1708    1767 
   Size:       1708      59
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     475    1767 
   Size:        475    1292
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1045    1077    1767 
   Size:       1045      32     690

  Fragments arranged by size: 

                1045     690      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     857    1459    1748    1767 
   Size:        857     602     289      19

  Fragments arranged by size: 

                 857     602     289      19
 
ApaLI G'TGCA_C

Cuts at:      0     520    1767 
   Size:        520    1247
 
ApoI r'AATT_y

Cuts at:      0    1504    1767 
   Size:       1504     263
 
AvaI C'yCGr_G

Cuts at:      0     591     688    1767 
   Size:        591      97    1079

  Fragments arranged by size: 

                1079     591      97
 
BamHI G'GATC_C

Cuts at:      0     824    1767 
   Size:        824     943
 
BanI G'GyrC_C

Cuts at:      0    1113    1767 
   Size:       1113     654
 
BanII G_rGCy'C

Cuts at:      0     695    1048    1767 
   Size:        695     353     719

  Fragments arranged by size: 

                 719     695     353
 
BbeI G_GCGC'C

Cuts at:      0    1117    1767 
   Size:       1117     650
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     621    1197    1767 
   Size:        621     576     570

  Fragments arranged by size: 

                 621     576     570
 
BbvCI CC'TCA_GC

Cuts at:      0    1005    1767 
   Size:       1005     762
 
BglI GCCn_nnn'nGGC

Cuts at:      0     190    1519    1767 
   Size:        190    1329     248

  Fragments arranged by size: 

                1329     248     190
 
BglII A'GATC_T

Cuts at:      0     926    1478    1767 
   Size:        926     552     289

  Fragments arranged by size: 

                 926     552     289
 
BlpI GC'TnA_GC

Cuts at:      0    1146    1151    1767 
   Size:       1146       5     616

  Fragments arranged by size: 

                1146     616       5
 
Bme1580I G_kGCm'C

Cuts at:      0     524     864    1435    1715    1767 
   Size:        524     340     571     280      52

  Fragments arranged by size: 

                 571     524     340     280      52
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     765    1767 
   Size:        765    1002
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     439     625     628     685     735    1177    1767 
   Size:        313     126     186       3      57      50     442     590

  Fragments arranged by size: 

                 590     442     313     186     126      57      50       3
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1005    1767 
   Size:       1005     762
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      13     601    1065    1534    1767 
   Size:         13     588     464     469     233

  Fragments arranged by size: 

                 588     469     464     233      13
 
BsaBI GATnn'nnATC

Cuts at:      0    1483    1767 
   Size:       1483     284
 
BsaHI Gr'CG_yC

Cuts at:      0     140     470    1114    1298    1767 
   Size:        140     330     644     184     469

  Fragments arranged by size: 

                 644     469     330     184     140
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      61      91    1045    1075    1396    1426    1767 
   Size:         61      30     954      30     321      30     341

  Fragments arranged by size: 

                 954     341     321      61      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     309     355     685     754    1767 
   Size:        309      46     330      69    1013

  Fragments arranged by size: 

                1013     330     309      69      46
 
BseYI C'CCAG_C

Cuts at:      0     979    1436    1767 
   Size:        979     457     331

  Fragments arranged by size: 

                 979     457     331
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262    1767 
   Size:        262    1505
 
BsiEI CG_ry'CG

Cuts at:      0     162    1013    1767 
   Size:        162     851     754

  Fragments arranged by size: 

                 851     754     162
 
BsiHKAI G_wGCw'C

Cuts at:      0     524     695    1767 
   Size:        524     171    1072

  Fragments arranged by size: 

                1072     524     171
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     523     753    1767 
   Size:        523     230    1014

  Fragments arranged by size: 

                1014     523     230
 
Bsp1286I G_dGCh'C

Cuts at:      0     524     695     864    1048    1435    1715    1767 
   Size:        524     171     169     184     387     280      52

  Fragments arranged by size: 

                 524     387     280     184     171     169      52
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     284     983    1766    1767 
   Size:        284     699     783       1

  Fragments arranged by size: 

                 783     699     284       1
 
BsrBI CCG'CTC

Cuts at:      0     621    1767 
   Size:        621    1146
 
BsrDI GCAATG_nn'

Cuts at:      0    1311    1767 
   Size:       1311     456
 
BsrFI r'CCGG_y

Cuts at:      0     485    1767 
   Size:        485    1282
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     125    1589    1767 
   Size:        125    1464     178

  Fragments arranged by size: 

                1464     178     125
 
BstEII G'GTnAC_C

Cuts at:      0     846    1767 
   Size:        846     921
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     447    1767 
   Size:        447    1320
 
BstYI r'GATC_y

Cuts at:      0     824     926    1478    1767 
   Size:        824     102     552     289

  Fragments arranged by size: 

                 824     552     289     102
 
BtgI C'CryG_G

Cuts at:      0     228     670    1140    1730    1767 
   Size:        228     442     470     590      37

  Fragments arranged by size: 

                 590     470     442     228      37
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     312    1767 
   Size:        312    1455
 
BtsI GCAGTG_nn'

Cuts at:      0     123     574    1319    1767 
   Size:        123     451     745     448

  Fragments arranged by size: 

                 745     451     448     123
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1719    1754    1767 
   Size:       1719      35      13

  Fragments arranged by size: 

                1719      35      13
 
DraIII CAC_nnn'GTG

Cuts at:      0     393    1767 
   Size:        393    1374
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      27    1767 
   Size:         27    1740
 
EaeI y'GGCC_r

Cuts at:      0     191     865    1137    1767 
   Size:        191     674     272     630

  Fragments arranged by size: 

                 674     630     272     191
 
EarI CTCTTCn'nnn_

Cuts at:      0      70    1767 
   Size:         70    1697
 
EcoICRI GAG'CTC

Cuts at:      0     693    1767 
   Size:        693    1074
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     439     625     628     685     735    1177    1351 
   Size:        313     126     186       3      57      50     442     174

Cuts at:   1351    1667    1767 
   Size:        316     100

  Fragments arranged by size: 

                 442     316     313     186     174     126     100      57
                  50       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     565     739    1767 
   Size:        565     174    1028

  Fragments arranged by size: 

                1028     565     174
 
EcoO109I rG'GnC_Cy

Cuts at:      0     759     804    1643    1767 
   Size:        759      45     839     124

  Fragments arranged by size: 

                 839     759     124      45
 
EcoRI G'AATT_C

Cuts at:      0    1504    1767 
   Size:       1504     263
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     699    1767 
   Size:        699    1068
 
HaeII r_GCGC'y

Cuts at:      0    1004    1117    1767 
   Size:       1004     113     650

  Fragments arranged by size: 

                1004     650     113
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     574     606    1087    1119    1360    1392    1767 
   Size:        574      32     481      32     241      32     375

  Fragments arranged by size: 

                 574     481     375     241      32      32      32
 
HincII GTy'rAC

Cuts at:      0     261    1767 
   Size:        261    1506
 
HindIII A'AGCT_T

Cuts at:      0     787    1767 
   Size:        787     980
 
KasI G'GCGC_C

Cuts at:      0    1113    1767 
   Size:       1113     654
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     464     752    1767 
   Size:        341     123     288    1015

  Fragments arranged by size: 

                1015     341     288     123
 
MscI TGG'CCA

Cuts at:      0    1139    1767 
   Size:       1139     628
 
MslI CAynn'nnrTG

Cuts at:      0    1708    1767 
   Size:       1708      59
 
MspA1I CmG'CkG

Cuts at:      0    1150    1459    1767 
   Size:       1150     309     308

  Fragments arranged by size: 

                1150     309     308
 
NaeI GCC'GGC

Cuts at:      0     487    1767 
   Size:        487    1280
 
NarI GG'CG_CC

Cuts at:      0    1114    1767 
   Size:       1114     653
 
NcoI C'CATG_G

Cuts at:      0     228    1140    1767 
   Size:        228     912     627

  Fragments arranged by size: 

                 912     627     228
 
NgoMIV G'CCGG_C

Cuts at:      0     485    1767 
   Size:        485    1282
 
NspI r_CATG'y

Cuts at:      0     132     378     513    1587    1767 
   Size:        132     246     135    1074     180

  Fragments arranged by size: 

                1074     246     180     135     132
 
PasI CC'CwG_GG

Cuts at:      0    1255    1767 
   Size:       1255     512
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1268    1550    1599    1767 
   Size:       1268     282      49     168

  Fragments arranged by size: 

                1268     282     168      49
 
PfoI T'CCnGG_A

Cuts at:      0     266     416    1767 
   Size:        266     150    1351

  Fragments arranged by size: 

                1351     266     150
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     205     237    1045    1077    1767 
   Size:        205      32     808      32     690

  Fragments arranged by size: 

                 808     690     205      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1643    1767 
   Size:       1643     124
 
PstI C_TGCA'G

Cuts at:      0     383     740     833    1767 
   Size:        383     357      93     934

  Fragments arranged by size: 

                 934     383     357      93
 
PvuI CG_AT'CG

Cuts at:      0    1013    1767 
   Size:       1013     754
 
PvuII CAG'CTG

Cuts at:      0    1150    1767 
   Size:       1150     617
 
SacI G_AGCT'C

Cuts at:      0     695    1767 
   Size:        695    1072
 
SanDI GG'GwC_CC

Cuts at:      0    1643    1767 
   Size:       1643     124
 
SbfI CC_TGCA'GG

Cuts at:      0     740    1767 
   Size:        740    1027
 
SexAI A'CCwGG_T

Cuts at:      0     932    1767 
   Size:        932     835
 
SfcI C'TryA_G

Cuts at:      0     379     736     829    1767 
   Size:        379     357      93     938

  Fragments arranged by size: 

                 938     379     357      93
 
SfoI GGC'GCC

Cuts at:      0    1115    1767 
   Size:       1115     652
 
SmlI C'TyrA_G

Cuts at:      0     591    1767 
   Size:        591    1176
 
StuI AGG'CCT

Cuts at:      0      76    1767 
   Size:         76    1691
 
StyI C'CwwG_G

Cuts at:      0     228     566     950     965    1064    1106    1140    1379 
   Size:        228     338     384      15      99      42      34     239

Cuts at:   1379    1441    1767 
   Size:         62     326

  Fragments arranged by size: 

                 384     338     326     239     228      99      62      42
                  34      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     625     639    1065    1767 
   Size:        625      14     426     702

  Fragments arranged by size: 

                 702     625     426      14
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      61      93     662     694    1396    1428    1767 
   Size:         61      32     569      32     702      32     339

  Fragments arranged by size: 

                 702     569     339      61      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     256    1767 
   Size:        256    1511
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     792    1767 
   Size:        792     975
 
XhoI C'TCGA_G

Cuts at:      0     591    1767 
   Size:        591    1176
 
XmnI GAAnn'nnTTC

Cuts at:      0     430     994    1767 
   Size:        430     564     773

  Fragments arranged by size: 

                 773     564     430
 
ZraI GAC'GTC

Cuts at:      0     141    1767 
   Size:        141    1626
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AhdI     AleI     AlfI     AloI    AlwNI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BglI    BglII     BlpI Bme1580I     BplI     BpmI   Bpu10I
     BsaI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BstAPI
   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI   DraIII
     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI    HaeII    Hin4I   HincII  HindIII     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
    PflMI     PfoI     PpiI    PpuMI     PstI     PvuI    PvuII     SacI
    SanDI     SbfI    SexAI     SfcI     SfoI     SmlI     StuI     StyI
    TaqII     TstI  Tth111I     XcmI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     ApaI
     AscI     AseI    AsiSI    AvrII     BaeI     BcgI    BciVI     BclI
    BfrBI    BmgBI     BmrI     BmtI    BpuEI    BsaAI    BsaWI    BsiWI
     BsmI    BspEI    BspHI    BsrGI   BssHII    BssSI    BstBI  BstZ17I
   Bsu36I     ClaI     DraI     EagI     EciI    EcoRV     FseI     FspI
    FspAI     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI    RsrII    SacII     SalI     SapI     ScaI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI     XbaI     XmaI
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