Restriction map for pF1KE9630
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AocI        
Aor51HI     AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BfrI        BglI        BlnI        BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsoBI       BspEI       BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      Cfr10I      CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     FseI        FspI        
Hin1I       HincII      HindII      HpaI        KasI        
KpnI        KspI        MluI        MroI        MspA1I      
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SpeI        Sse8387I    SwaI        Tth111I     
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI         1821  GTCTAC               GT^[AC][GT]AC
ApaI         3200  GGGCCC               GGGCC^C
BalI         1199  TGGCCA               TGG^CCA
BclI          632  TGATCA               T^GATCA
BcnI            9  CCCGG                CC^[GC]GG
BglII        2421  AGATCT               A^GATCT
BlpI         2928  GCTCAGC              GC^T[ACGT]AGC
BpmI          581  CTGGAG               CTGGAG(16/14)
Bpu1102I     2928  GCTCAGC              GC^T[ACGT]AGC
BsaBI        3118  GATTGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        2000  CGTCTC               CGTCTC(1/5)
BsmI         2699  GAATGC               GAATGC(1/-1)
BspDI        2956  ATCGAT               AT^CGAT
BstAPI       1810  GCACAACTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         273  CCATGGGATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        2928  GCTCAGC              GC^T[ACGT]AGC
CfoI         1009  GCGC                 GCG^C
ClaI         2956  ATCGAT               AT^CGAT
DraIII       1250  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1199  TGGCCA               [CT]^GGCC[GA]
EcoNI        2041  CCTGCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         588  GATATC               GAT^ATC
EcoT22I      1493  ATGCAT               ATGCA^T
FbaI          632  TGATCA               T^GATCA
HaeII        1008  AGCGCC               [GA]GCGC^[CT]
HapII          10  CCGG                 C^CGG
HhaI         1009  GCGC                 GCG^C
HinP1I       1009  GCGC                 G^CGC
HpaII          10  CCGG                 C^CGG
MamI         3118  GATTGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2341  CAATTG               C^AATTG
MluNI        1199  TGGCCA               TGG^CCA
MscI         1199  TGGCCA               TGG^CCA
MspI           10  CCGG                 C^CGG
MunI         2341  CAATTG               C^AATTG
NciI            9  CCCGG                CC^[GC]GG
NsiI         1493  ATGCAT               ATGCA^T
PshAI        2836  GACCTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         2599  GAGCTC               GAGCT^C
ScaI         1231  AGTACT               AGT^ACT
SnaBI        3206  TACGTA               TAC^GTA
StuI         1991  AGGCCT               AGG^CCT
TaqI         2957  TCGA                 T^CGA
Tsp45I        239  GTGAC                ^GT[GC]AC
TthHB8I      2957  TCGA                 T^CGA
XbaI         1476  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AciI          935  CCGC                 CCGC(-3/-1)
              316  GCGG                
AflIII       2006  ACATGT               A^C[GA][CT]GT
             2540  ACATGT              
AlwI         2128  GATCC                GGATC(4/5)
              927  GGATC               
AlwNI         997  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2102  CAGACACTG           
Asp700I      2734  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3088  GAATTTTTTC          
BbsI          811  GAAGAC               GAAGAC(2/6)
             1016  GTCTTC              
BpuAI         811  GAAGAC               GAAGAC(2/6)
             1016  GTCTTC              
BsaAI         968  CACGTA               [CT]AC^GT[GA]
             3206  TACGTA              
BseRI        1148  CTCCTC               GAGGAG(10/8)
             3029  GAGGAG              
BsgI         1808  CTGCAC               GTGCAG(16/14)
             1366  GTGCAG              
Bsp1407I      266  TGTACA               T^GTACA
              302  TGTACA              
BspHI        1236  TCATGA               T^CATGA
             1412  TCATGA              
BspLU11I     2006  ACATGT               A^CATGT
             2540  ACATGT              
BspMI         696  GCAGGT               ACCTGC(4/8)
             2047  GCAGGT              
BsrGI         266  TGTACA               T^GTACA
              302  TGTACA              
FauI          934  CCCGC                CCCGC(4/6)
              316  GCGGG               
HgaI         2110  GACGC                GACGC(5/10)
             1819  GCGTC               
HindIII       721  AAGCTT               A^AGCTT
             1837  AAGCTT              
MaeII         969  ACGT                 A^CGT
             3207  ACGT                
PpuMI        1651  AGGACCT              [GA]G^G[AT]CC[CT]
             2500  GGGACCC             
RcaI         1236  TCATGA               T^CATGA
             1412  TCATGA              
SmlI          461  CTCAAG               C^T[CT][GA]AG
             2669  CTCAAG              
SphI          348  GCATGC               GCATG^C
             2990  GCATGC              
SspBI         266  TGTACA               T^GTACA
              302  TGTACA              
SspI          653  AATATT               AAT^ATT
             3109  AATATT              
XcmI         1187  CCATGTTTGCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2367  CCATCACCTTCTTGG     
XmnI         2734  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3088  GAATTTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AcsI        AfaI        AluI        Alw44I      ApaLI       
ApoI        AspHI       AvaII       BanI        BanII       
BbvI        BfaI        BmyI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EarI        EcoO109I    
EcoRI       EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MvaI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
Van91I      XhoII       
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