Restriction map for pF1KE9630
 (Linear) (Six-base) MAPSORT of: KIEE9630.seq  Check: 24  from: 1  to: 3255



KIEE9630.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 19:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2038    3255 
   Size:       2038    1217
 
AccI GT'mk_AC

Cuts at:      0    1822    3255 
   Size:       1822    1433
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     349     578    1736    3065    3181    3255 
   Size:          7     342     229    1158    1329     116      74

  Fragments arranged by size: 

                1329    1158     342     229     116      74       7
 
AflIII A'CryG_T

Cuts at:      0    2006    2540    3255 
   Size:       2006     534     715

  Fragments arranged by size: 

                2006     715     534
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     371    3255 
   Size:        371    2884
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1002    2107    3255 
   Size:       1002    1105    1148

  Fragments arranged by size: 

                1148    1105    1002
 
ApaI G_GGCC'C

Cuts at:      0    3204    3255 
   Size:       3204      51
 
ApaLI G'TGCA_C

Cuts at:      0     661    1384    1855    3255 
   Size:        661     723     471    1400

  Fragments arranged by size: 

                1400     723     661     471
 
ApoI r'AATT_y

Cuts at:      0     108     163     220     374     742    1240    1957    2335 
   Size:        108      55      57     154     368     498     717     378

Cuts at:   2335    2449    2488    3088    3255 
   Size:        114      39     600     167

  Fragments arranged by size: 

                 717     600     498     378     368     167     154     114
                 108      57      55      39
 
BanI G'GyrC_C

Cuts at:      0    1071    1770    1938    3255 
   Size:       1071     699     168    1317

  Fragments arranged by size: 

                1317    1071     699     168
 
BanII G_rGCy'C

Cuts at:      0     768    1789    2153    2603    3204    3255 
   Size:        768    1021     364     450     601      51

  Fragments arranged by size: 

                1021     768     601     450     364      51
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     818    1009    3255 
   Size:        818     191    2246

  Fragments arranged by size: 

                2246     818     191
 
BclI T'GATC_A

Cuts at:      0     632    3255 
   Size:        632    2623
 
BfrBI ATG'CAT

Cuts at:      0    1495    3255 
   Size:       1495    1760
 
BglII A'GATC_T

Cuts at:      0    2421    3255 
   Size:       2421     834
 
BlpI GC'TnA_GC

Cuts at:      0    2929    3255 
   Size:       2929     326
 
Bme1580I G_kGCm'C

Cuts at:      0     665    1388    1859    3204    3255 
   Size:        665     723     471    1345      51

  Fragments arranged by size: 

                1345     723     665     471      51
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1345    1500    1769    2499    3255 
   Size:       1345     155     269     730     756

  Fragments arranged by size: 

                1345     756     730     269     155
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1163    3255 
   Size:       1163    2092
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     602    3255 
   Size:        602    2653
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     446    2654    3255 
   Size:        446    2208     601

  Fragments arranged by size: 

                2208     601     446
 
BsaAI yAC'GTr

Cuts at:      0     970    3208    3255 
   Size:        970    2238      47

  Fragments arranged by size: 

                2238     970      47
 
BsaBI GATnn'nnATC

Cuts at:      0    3122    3255 
   Size:       3122     133
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1139    3044    3255 
   Size:       1139    1905     211

  Fragments arranged by size: 

                1905    1139     211
 
BseYI C'CCAG_C

Cuts at:      0     992    1125    2146    3255 
   Size:        992     133    1021    1109

  Fragments arranged by size: 

                1109    1021     992     133
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1387    1793    3255 
   Size:       1387     406    1462

  Fragments arranged by size: 

                1462    1387     406
 
BsiHKAI G_wGCw'C

Cuts at:      0     665    1388    1859    2603    3255 
   Size:        665     723     471     744     652

  Fragments arranged by size: 

                 744     723     665     652     471
 
BsmI GAATG_Cn'

Cuts at:      0    2705    3255 
   Size:       2705     550
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2006    3255 
   Size:       2006    1249
 
Bsp1286I G_dGCh'C

Cuts at:      0     665     768    1388    1789    1859    2153    2603    3204 
   Size:        665     103     620     401      70     294     450     601

Cuts at:   3204    3255 
   Size:         51

  Fragments arranged by size: 

                 665     620     601     450     401     294     103      70
                  51
 
BspHI T'CATG_A

Cuts at:      0    1236    1412    3255 
   Size:       1236     176    1843

  Fragments arranged by size: 

                1843    1236     176
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     687    2038    3255 
   Size:        687    1351    1217

  Fragments arranged by size: 

                1351    1217     687
 
BsrDI GCAATG_nn'

Cuts at:      0      66     305     805    1142    2117    2308    3255 
   Size:         66     239     500     337     975     191     947

  Fragments arranged by size: 

                 975     947     500     337     239     191      66
 
BsrGI T'GTAC_A

Cuts at:      0     266     302    3255 
   Size:        266      36    2953

  Fragments arranged by size: 

                2953     266      36
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1816    3255 
   Size:       1816    1439
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     280    3255 
   Size:        280    2975
 
BstYI r'GATC_y

Cuts at:      0     927    2127    2421    3255 
   Size:        927    1200     294     834

  Fragments arranged by size: 

                1200     927     834     294
 
BtgI C'CryG_G

Cuts at:      0     273    2221    2941    3255 
   Size:        273    1948     720     314

  Fragments arranged by size: 

                1948     720     314     273
 
BtsI GCAGTG_nn'

Cuts at:      0    1202    2589    3255 
   Size:       1202    1387     666

  Fragments arranged by size: 

                1387    1202     666
 
ClaI AT'CG_AT

Cuts at:      0    2957    3255 
   Size:       2957     298
 
DraI TTT'AAA

Cuts at:      0     218     503    2459    3255 
   Size:        218     285    1956     796

  Fragments arranged by size: 

                1956     796     285     218
 
DraIII CAC_nnn'GTG

Cuts at:      0    1255    3255 
   Size:       1255    2000
 
EaeI y'GGCC_r

Cuts at:      0    1199    3255 
   Size:       1199    2056
 
EarI CTCTTCn'nnn_

Cuts at:      0      98     621    1566    1712    3255 
   Size:         98     523     945     146    1543

  Fragments arranged by size: 

                1543     945     523     146      98
 
EcoICRI GAG'CTC

Cuts at:      0    2601    3255 
   Size:       2601     654
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       7     349     578     602    1736    3065    3181    3255 
   Size:          7     342     229      24    1134    1329     116      74

  Fragments arranged by size: 

                1329    1134     342     229     116      74      24       7
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2045    3255 
   Size:       2045    1210
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1652    2501    3200    3201    3255 
   Size:       1652     849     699       1      54

  Fragments arranged by size: 

                1652     849     699      54       1
 
EcoRI G'AATT_C

Cuts at:      0     374    1240    2488    3255 
   Size:        374     866    1248     767

  Fragments arranged by size: 

                1248     866     767     374
 
EcoRV GAT'ATC

Cuts at:      0     590    3255 
   Size:        590    2665
 
HaeII r_GCGC'y

Cuts at:      0    1012    3255 
   Size:       1012    2243
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     595     627    1250    1282    1786    1818    2265    2297 
   Size:        595      32     623      32     504      32     447      32

Cuts at:   2297    2797    2829    3255 
   Size:        500      32     426

  Fragments arranged by size: 

                 623     595     504     500     447     426      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     721    1837    3255 
   Size:        721    1116    1418

  Fragments arranged by size: 

                1418    1116     721
 
MfeI C'AATT_G

Cuts at:      0    2341    3255 
   Size:       2341     914
 
MscI TGG'CCA

Cuts at:      0    1201    3255 
   Size:       1201    2054
 
MslI CAynn'nnrTG

Cuts at:      0     353    1417    1936    2545    2729    3007    3013    3255 
   Size:        353    1064     519     609     184     278       6     242

  Fragments arranged by size: 

                1064     609     519     353     278     242     184       6
            
 
NcoI C'CATG_G

Cuts at:      0     273    2221    2941    3255 
   Size:        273    1948     720     314

  Fragments arranged by size: 

                1948     720     314     273
 
NsiI A_TGCA'T

Cuts at:      0    1497    3255 
   Size:       1497    1758
 
NspI r_CATG'y

Cuts at:      0      45     352    2010    2544    2994    3255 
   Size:         45     307    1658     534     450     261

  Fragments arranged by size: 

                1658     534     450     307     261      45
 
PasI CC'CwG_GG

Cuts at:      0    2415    2483    3255 
   Size:       2415      68     772

  Fragments arranged by size: 

                2415     772      68
 
PciI A'CATG_T

Cuts at:      0    2006    2540    3255 
   Size:       2006     534     715

  Fragments arranged by size: 

                2006     715     534
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1255    2483    2511    3255 
   Size:       1255    1228      28     744

  Fragments arranged by size: 

                1255    1228     744      28
 
PfoI T'CCnGG_A

Cuts at:      0     531    3255 
   Size:        531    2724
 
PpuMI rG'GwC_Cy

Cuts at:      0    1652    2501    3255 
   Size:       1652     849     754

  Fragments arranged by size: 

                1652     849     754
 
PshAI GACnn'nnGTC

Cuts at:      0    2840    3255 
   Size:       2840     415
 
PspOMI G'GGCC_C

Cuts at:      0    3200    3255 
   Size:       3200      55
 
PstI C_TGCA'G

Cuts at:      0     347    1113    2049    3255 
   Size:        347     766     936    1206

  Fragments arranged by size: 

                1206     936     766     347
 
SacI G_AGCT'C

Cuts at:      0    2603    3255 
   Size:       2603     652
 
SanDI GG'GwC_CC

Cuts at:      0    2501    3255 
   Size:       2501     754
 
ScaI AGT'ACT

Cuts at:      0    1233    3255 
   Size:       1233    2022
 
SfcI C'TryA_G

Cuts at:      0     343    1109    2045    2604    3255 
   Size:        343     766     936     559     651

  Fragments arranged by size: 

                 936     766     651     559     343
 
SmlI C'TyrA_G

Cuts at:      0     461    2669    3255 
   Size:        461    2208     586

  Fragments arranged by size: 

                2208     586     461
 
SnaBI TAC'GTA

Cuts at:      0    3208    3255 
   Size:       3208      47
 
SphI G_CATG'C

Cuts at:      0     352    2994    3255 
   Size:        352    2642     261

  Fragments arranged by size: 

                2642     352     261
 
SspI AAT'ATT

Cuts at:      0     655    3111    3255 
   Size:        655    2456     144

  Fragments arranged by size: 

                2456     655     144
 
StuI AGG'CCT

Cuts at:      0    1993    3255 
   Size:       1993    1262
 
StyI C'CwwG_G

Cuts at:      0     273     333    2221    2941    3255 
   Size:        273      60    1888     720     314

  Fragments arranged by size: 

                1888     720     314     273      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1392    1788    3255 
   Size:       1392     396    1467

  Fragments arranged by size: 

                1467    1392     396
 
TatI w'GTAC_w

Cuts at:      0     230     266     302    1231    3222    3255 
   Size:        230      36      36     929    1991      33

  Fragments arranged by size: 

                1991     929     230      36      36      33
 
XbaI T'CTAG_A

Cuts at:      0    1476    3255 
   Size:       1476    1779
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1194    2374    3255 
   Size:       1194    1180     881

  Fragments arranged by size: 

                1194    1180     881
 
XmnI GAAnn'nnTTC

Cuts at:      0    2738    3092    3255 
   Size:       2738     354     163

  Fragments arranged by size: 

                2738     354     163
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AlfI    AlwNI     ApaI    ApaLI
     ApoI     BanI    BanII     BbsI     BclI    BfrBI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI    BsaAI    BsaBI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrDI    BsrGI   BstAPI    BstXI    BstYI     BtgI     BtsI     ClaI
     DraI   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV    HaeII    Hin4I  HindIII     MfeI     MscI     MslI
     NcoI     NsiI     NspI     PasI     PciI    PflMI     PfoI    PpuMI
    PshAI   PspOMI     PstI     SacI    SanDI     ScaI     SfcI     SmlI
    SnaBI     SphI     SspI     StuI     StyI    TaqII     TatI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AloI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI    BciVI     BglI    BmgBI     BmtI   Bpu10I
     BsaI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI    BspEI    BsrBI
    BsrFI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
    CspCI     DrdI     EagI     EciI     FalI     FseI     FspI    FspAI
   HincII     HpaI     KasI     KpnI     MluI     MmeI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PmeI
     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SpeI     SrfI     SwaI     TstI  Tth111I     XhoI
     XmaI     ZraI
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