Restriction map for
pF1KB5768
(Linear) (Six-base) MAPSORT of: KIBB5768.seq Check: 8204 from: 1 to: 2280
KIBB5768.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 1976 2075 2280
Size: 1976 99 205
Fragments arranged by size:
1976 205 99
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 151 951 2280
Size: 122 29 800 1329
Fragments arranged by size:
1329 800 122 29
AflIII A'CryG_T
Cuts at: 0 1967 2280
Size: 1967 313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1410 2280
Size: 1410 870
ApaLI G'TGCA_C
Cuts at: 0 2113 2280
Size: 2113 167
ApoI r'AATT_y
Cuts at: 0 415 655 673 1240 1816 2034 2280
Size: 415 240 18 567 576 218 246
Fragments arranged by size:
576 567 415 246 240 218 18
BanI G'GyrC_C
Cuts at: 0 1340 2280
Size: 1340 940
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 980 1799 2280
Size: 10 970 819 481
Fragments arranged by size:
970 819 481 10
BciVI GTATCCnnnnn_n'
Cuts at: 0 1610 1873 2280
Size: 1610 263 407
Fragments arranged by size:
1610 407 263
BclI T'GATC_A
Cuts at: 0 1443 2280
Size: 1443 837
Bme1580I G_kGCm'C
Cuts at: 0 2117 2280
Size: 2117 163
BmrI ACTGGGnnnn_n'
Cuts at: 0 603 2280
Size: 603 1677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 1928 1935 2280
Size: 785 1143 7 345
Fragments arranged by size:
1143 785 345 7
Bpu10I CC'TnA_GC
Cuts at: 0 403 2280
Size: 403 1877
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 846 2041 2280
Size: 846 1195 239
Fragments arranged by size:
1195 846 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 647 2002 2280
Size: 647 1355 278
Fragments arranged by size:
1355 647 278
BsaAI yAC'GTr
Cuts at: 0 890 2280
Size: 890 1390
BsaBI GATnn'nnATC
Cuts at: 0 2015 2280
Size: 2015 265
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 404 434 1868 1898 2280
Size: 404 30 1434 30 382
Fragments arranged by size:
1434 404 382 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1565 1741 2280
Size: 1565 176 539
Fragments arranged by size:
1565 539 176
BseYI C'CCAG_C
Cuts at: 0 1773 1955 2280
Size: 1773 182 325
Fragments arranged by size:
1773 325 182
BsiHKAI G_wGCw'C
Cuts at: 0 123 2027 2117 2280
Size: 123 1904 90 163
Fragments arranged by size:
1904 163 123 90
BsmI GAATG_Cn'
Cuts at: 0 300 2280
Size: 300 1980
Bsp1286I G_dGCh'C
Cuts at: 0 123 2027 2117 2280
Size: 123 1904 90 163
Fragments arranged by size:
1904 163 123 90
BspHI T'CATG_A
Cuts at: 0 1428 2280
Size: 1428 852
BsrDI GCAATG_nn'
Cuts at: 0 414 857 1242 1703 2183 2280
Size: 414 443 385 461 480 97
Fragments arranged by size:
480 461 443 414 385 97
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2137 2280
Size: 2137 143
BstYI r'GATC_y
Cuts at: 0 531 2280
Size: 531 1749
BtgI C'CryG_G
Cuts at: 0 2091 2199 2280
Size: 2091 108 81
Fragments arranged by size:
2091 108 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 734 2280
Size: 734 1546
BtsI GCAGTG_nn'
Cuts at: 0 925 1766 1943 2266 2280
Size: 925 841 177 323 14
Fragments arranged by size:
925 841 323 177 14
ClaI AT'CG_AT
Cuts at: 0 800 2280
Size: 800 1480
DraI TTT'AAA
Cuts at: 0 143 2280
Size: 143 2137
EarI CTCTTCn'nnn_
Cuts at: 0 555 599 2280
Size: 555 44 1681
Fragments arranged by size:
1681 555 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 151 785 951 1928 1935 2280
Size: 122 29 634 166 977 7 345
Fragments arranged by size:
977 634 345 166 122 29 7
EcoO109I rG'GnC_Cy
Cuts at: 0 826 2280
Size: 826 1454
EcoRI G'AATT_C
Cuts at: 0 2034 2280
Size: 2034 246
EcoRV GAT'ATC
Cuts at: 0 1673 2280
Size: 1673 607
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 217 1620 2280
Size: 217 1403 660
Fragments arranged by size:
1403 660 217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1557 1589 1662 1694 2280
Size: 1557 32 73 32 586
Fragments arranged by size:
1557 586 73 32 32
HindIII A'AGCT_T
Cuts at: 0 358 2280
Size: 358 1922
MluI A'CGCG_T
Cuts at: 0 1967 2280
Size: 1967 313
MslI CAynn'nnrTG
Cuts at: 0 1825 2239 2280
Size: 1825 414 41
Fragments arranged by size:
1825 414 41
MspA1I CmG'CkG
Cuts at: 0 1506 1807 1955 2280
Size: 1506 301 148 325
Fragments arranged by size:
1506 325 301 148
NdeI CA'TA_TG
Cuts at: 0 489 666 1858 2280
Size: 489 177 1192 422
Fragments arranged by size:
1192 489 422 177
PflMI CCAn_nnn'nTGG
Cuts at: 0 763 2280
Size: 763 1517
PfoI T'CCnGG_A
Cuts at: 0 1460 2280
Size: 1460 820
PmlI CAC'GTG
Cuts at: 0 890 2280
Size: 890 1390
PpuMI rG'GwC_Cy
Cuts at: 0 826 2280
Size: 826 1454
PsiI TTA'TAA
Cuts at: 0 161 236 2280
Size: 161 75 2044
Fragments arranged by size:
2044 161 75
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2215 2247 2280
Size: 2215 32 33
Fragments arranged by size:
2215 33 32
PstI C_TGCA'G
Cuts at: 0 216 1636 2280
Size: 216 1420 644
Fragments arranged by size:
1420 644 216
PvuII CAG'CTG
Cuts at: 0 1506 1807 1955 2280
Size: 1506 301 148 325
Fragments arranged by size:
1506 325 301 148
ScaI AGT'ACT
Cuts at: 0 230 1381 2280
Size: 230 1151 899
Fragments arranged by size:
1151 899 230
SfcI C'TryA_G
Cuts at: 0 212 1272 1632 2280
Size: 212 1060 360 648
Fragments arranged by size:
1060 648 360 212
SmlI C'TyrA_G
Cuts at: 0 861 2020 2280
Size: 861 1159 260
Fragments arranged by size:
1159 861 260
SspI AAT'ATT
Cuts at: 0 240 575 747 1100 1348 1963 2062 2280
Size: 240 335 172 353 248 615 99 218
Fragments arranged by size:
615 353 335 248 240 218 172 99
StyI C'CwwG_G
Cuts at: 0 1709 1737 2280
Size: 1709 28 543
Fragments arranged by size:
1709 543 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 936 1765 2251 2280
Size: 936 829 486 29
Fragments arranged by size:
936 829 486 29
TatI w'GTAC_w
Cuts at: 0 228 1379 2280
Size: 228 1151 901
Fragments arranged by size:
1151 901 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2191 2223 2280
Size: 2191 32 57
Fragments arranged by size:
2191 57 32
XbaI T'CTAG_A
Cuts at: 0 339 2280
Size: 339 1941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1952 2280
Size: 1952 328
XmnI GAAnn'nnTTC
Cuts at: 0 153 200 2280
Size: 153 47 2080
Fragments arranged by size:
2080 153 47
Enzymes that do cut:
AccI AcuI AflIII AlfI ApaLI ApoI BanI BbsI
BciVI BclI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspHI BsrDI BstAPI BstYI BtgI BtgZI BtsI ClaI
DraI EarI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I
HindIII MluI MslI MspA1I NdeI PflMI PfoI PmlI
PpuMI PsiI PsrI PstI PvuII ScaI SfcI SmlI
SspI StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BsaHI BsaWI
BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MmeI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PpiI PshAI PspOMI PspXI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XhoI XmaI ZraI