Restriction map for pF1KE1050
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BbeI        BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BpuAI       BsaBI       
BseAI       BsgI        BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HinfI       
HpaI        KasI        KpnI        MaeI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NarI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmeI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SalI        SapI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        SspBI       SspI        SwaI        TfiI        
Tru9I       Tth111I     Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AcyI          280  GACGCC               G[GA]^CG[CT]C
AlwNI        1380  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           52  CCTCAGG              CC^T[ACGT]AGG
BamHI        1046  GGATCC               G^GATCC
BlpI          297  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      297  GCTGAGC              GC^T[ACGT]AGC
BsaHI         280  GACGCC               G[GA]^CG[CT]C
BsaI          687  GGTCTC               GGTCTC(1/5)
BsmBI         437  CGTCTC               CGTCTC(1/5)
BspMI        1090  ACCTGC               ACCTGC(4/8)
BsrBI         427  CCGCTC               CCGCTC(-3/-3)
BstAPI       1370  GCAAGGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         831  CCAGTGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I         52  CCTCAGG              CC^T[ACGT]AGG
CelII         297  GCTGAGC              GC^T[ACGT]AGC
Eco81I         52  CCTCAGG              CC^T[ACGT]AGG
EcoT14I       213  CCATGG               C^C[AT][AT]GG
HaeII        1381  AGCGCC               [GA]GCGC^[CT]
Hin1I         280  GACGCC               G[GA]^CG[CT]C
MslI         1089  CACCTGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          195  GCCGGC               GCC^GGC
NcoI          213  CCATGG               C^CATGG
NdeI          365  CATATG               CA^TATG
NgoMIV        195  GCCGGC               G^CCGGC
NspI          925  ACATGC               [GA]CATG^[CT]
PpuMI         208  GGGTCCC              [GA]G^G[AT]CC[CT]
SacI         1399  GAGCTC               GAGCT^C
SbfI          697  CCTGCAGG             CCTGCA^GG
Sse8387I      697  CCTGCAGG             CCTGCA^GG
StyI          213  CCATGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AfaI          220  GTAC                 GT^AC
              822  GTAC                
AvaI           81  CCCGGG               C^[CT]CG[GA]G
              585  CCCGGG              
BalI          828  TGGCCA               TGG^CCA
              998  TGGCCA              
BanI           76  GGCACC               G^G[CT][GA]CC
              735  GGTGCC              
BbrPI         901  CACGTG               CAC^GTG
             1020  CACGTG              
BsaWI         180  ACCGGA               [AT]^CCGG[AT]
             1043  ACCGGA              
BsmFI         177  GGGAC                GGGAC(10/14)
              210  GTCCC               
BsoBI          81  CCCGGG               C^[CT]CG[GA]G
              585  CCCGGG              
BsrFI          93  ACCGGC               [GA]^CCGG[CT]
              195  GCCGGC              
BsrI          529  CCAGT                ACTGG(1/-1)
              831  CCAGT               
BstYI        1046  GGATCC               [GA]^GATC[CT]
             1151  AGATCC              
CfoI         1382  GCGC                 GCG^C
             1389  GCGC                
Cfr10I         93  ACCGGC               [GA]^CCGG[CT]
              195  GCCGGC              
EarI          704  GAAGAG               CTCTTC(1/4)
              710  GAAGAG              
EcoNI         601  CCTCTCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1222  CCTGAGCCAGG         
HhaI         1382  GCGC                 GCG^C
             1389  GCGC                
HinP1I       1382  GCGC                 G^CGC
             1389  GCGC                
Ksp632I       704  GAAGAG               CTCTTC(1/4)
              710  GAAGAG              
KspI           72  CCGCGG               CCGC^GG
             1107  CCGCGG              
MflI         1046  GGATCC               [GA]^GATC[CT]
             1151  AGATCC              
MluNI         828  TGGCCA               TGG^CCA
              998  TGGCCA              
MscI          828  TGGCCA               TGG^CCA
              998  TGGCCA              
PmaCI         901  CACGTG               CAC^GTG
             1020  CACGTG              
PmlI          901  CACGTG               CAC^GTG
             1020  CACGTG              
RsaI          220  GTAC                 GT^AC
              822  GTAC                
SacII          72  CCGCGG               CCGC^GG
             1107  CCGCGG              
ScaI          219  AGTACT               AGT^ACT
              821  AGTACT              
SmaI           81  CCCGGG               CCC^GGG
              585  CCCGGG              
TaqI         1115  TCGA                 T^CGA
             1266  TCGA                
Tsp509I       133  AATT                 ^AATT
             1251  AATT                
TthHB8I      1115  TCGA                 T^CGA
             1266  TCGA                
XhoII        1046  GGATCC               [GA]^GATC[CT]
             1151  AGATCC              
XmaCI          81  CCCGGG               C^CCGGG
              585  CCCGGG              
XmaI           81  CCCGGG               C^CCGGG
              585  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        ApaI        
AspHI       AvaII       BanII       BbvI        BcnI        
BglI        BmyI        BpmI        BsaAI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BssKI       BstF5I      BstNI       BstUI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PstI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StuI        TseI        
Tsp45I      TspRI       
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