Restriction map for pF1KE1047
 (Circular) (Six-base) MAPSORT of: pF1KE1047.seq  Check: 9115  from: 1  to: 4151



pF1KE1047  4151 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1082    1082 
   Size:       4151
 
AccI GT'mk_AC

Cuts at:    521    1189     521 
   Size:        668    3483
 
Acc65I G'GTAC_C

Cuts at:   1167    1167 
   Size:       4151
 
AclI AA'CG_TT

Cuts at:   3991    3991 
   Size:       4151
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    601    1976    2408    3155    3423     601 
   Size:       1375     432     747     268    1329

  Fragments arranged by size: 

                1375    1329     747     432     268
 
AflIII A'CryG_T

Cuts at:    942    1503    2607    3584     942 
   Size:        561    1104     977    1509

  Fragments arranged by size: 

                1509    1104     977     561
 
AgeI A'CCGG_T

Cuts at:   2539    2539 
   Size:       4151
 
AlwNI CAG_nnn'CTG

Cuts at:    351     819     984    1456    3023     351 
   Size:        468     165     472    1567    1479

  Fragments arranged by size: 

                1567    1479     472     468     165
 
ApaLI G'TGCA_C

Cuts at:   2921    2921 
   Size:       4151
 
ApoI r'AATT_y

Cuts at:    772    1155    3504    3760     772 
   Size:        383    2349     256    1163

  Fragments arranged by size: 

                2349    1163     383     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4151
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4151
 
AvaI C'yCGr_G

Cuts at:    238     716    1171     238 
   Size:        478     455    3218

  Fragments arranged by size: 

                3218     478     455
 
BamHI G'GATC_C

Cuts at:   1176    1176 
   Size:       4151
 
BanI G'GyrC_C

Cuts at:   1167    1581    1829    1864    1167 
   Size:        414     248      35    3454

  Fragments arranged by size: 

                3454     414     248      35
 
BanII G_rGCy'C

Cuts at:    862    1165    2195     862 
   Size:        303    1030    2818

  Fragments arranged by size: 

                2818    1030     303
 
BbeI G_GCGC'C

Cuts at:   1585    1833    1585 
   Size:        248    3903
 
BbsI GAAGACnn'nnnn_

Cuts at:    451     542     451 
   Size:         91    4060
 
BciVI GTATCCnnnnn_n'

Cuts at:   2049    2810    4151    2049 
   Size:        761    1341    2049

  Fragments arranged by size: 

                2049    1341     761
 
BclI T'GATC_A

Cuts at:   1671    1671 
   Size:       4151
 
BglI GCCn_nnn'nGGC

Cuts at:    799     799 
   Size:       4151
 
BglII A'GATC_T

Cuts at:    641    1666    3703     641 
   Size:       1025    2037    1089

  Fragments arranged by size: 

                2037    1089    1025
 
BlpI GC'TnA_GC

Cuts at:    841    1263     841 
   Size:        422    3729
 
Bme1580I G_kGCm'C

Cuts at:   1776    1869    2925    1776 
   Size:         93    1056    3002

  Fragments arranged by size: 

                3002    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1131    1363    1444    1779    3923    1131 
   Size:        232      81     335    2144    1359

  Fragments arranged by size: 

                2144    1359     335     232      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2518    2518 
   Size:       4151
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    216     526     533     216 
   Size:        310       7    3834

  Fragments arranged by size: 

                3834     310       7
 
Bpu10I CC'TnA_GC

Cuts at:    352     352 
   Size:       4151
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1327    2206    2698    2996    1327 
   Size:        879     492     298    2482

  Fragments arranged by size: 

                2482     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3855    3855 
   Size:       4151
 
BsaAI yAC'GTr

Cuts at:    657    1504     657 
   Size:        847    3304
 
BsaBI GATnn'nnATC

Cuts at:      6     116     646    1665    3702       6 
   Size:        110     530    1019    2037     455

  Fragments arranged by size: 

                2037    1019     530     455     110
 
BsaHI Gr'CG_yC

Cuts at:    539    1582    1830    2532    4028     539 
   Size:       1043     248     702    1496     662

  Fragments arranged by size: 

                1496    1043     702     662     248
 
BsaWI w'CCGG_w

Cuts at:   1340    1861    2539    2813    2960    3371    1340 
   Size:        521     678     274     147     411    2120

  Fragments arranged by size: 

                2120     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    913    1074     913 
   Size:        161    3990
 
BseYI C'CCAG_C

Cuts at:    449     575     924     951    1038    1576    2334    2911    3407 
   Size:        126     349      27      87     538     758     577     496

Cuts at:   3407     449 
   Size:       1193

  Fragments arranged by size: 

                1193     758     577     538     496     349     126      87
                  27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    295     499     513     295 
   Size:        204      14    3933

  Fragments arranged by size: 

                3933     204      14
 
BsiEI CG_ry'CG

Cuts at:    104    1693    1739    2947     104 
   Size:       1589      46    1208    1308

  Fragments arranged by size: 

                1589    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    656    1061    1165    1943    2133    2925     656 
   Size:        405     104     778     190     792    1882

  Fragments arranged by size: 

                1882     792     778     405     190     104
 
Bsp1286I G_dGCh'C

Cuts at:    656     862    1061    1165    1776    1869    1943    2133    2195 
   Size:        206     199     104     611      93      74     190      62

Cuts at:   2195    2925     656 
   Size:        730    1882

  Fragments arranged by size: 

                1882     730     611     206     199     190     104      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   3371    3371 
   Size:       4151
 
BspHI T'CATG_A

Cuts at:   3327    3327 
   Size:       4151
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1082    1201    1415    1717    2098    1082 
   Size:        119     214     302     381    3135

  Fragments arranged by size: 

                3135     381     302     214     119
 
BsrBI CCG'CTC

Cuts at:     50     327    2443    2497    3981    4146      50 
   Size:        277    2116      54    1484     165      55

  Fragments arranged by size: 

                2116    1484     277     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2063    2063 
   Size:       4151
 
BsrFI r'CCGG_y

Cuts at:   1004    2149    2330    2539    1004 
   Size:       1145     181     209    2616

  Fragments arranged by size: 

                2616    1145     209     181
 
BssSI C'ACGA_G

Cuts at:   2422    2780    2422 
   Size:        358    3793
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1528    1528 
   Size:       4151
 
BstBI TT'CG_AA

Cuts at:   2512    2512 
   Size:       4151
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114     114 
   Size:       4151
 
BstYI r'GATC_y

Cuts at:      1     117     641    1176    1643    1666    2001    2247    3259 
   Size:        116     524     535     467      23     335     246    1012

Cuts at:   3259    3345    3357    3703       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     535     524     467     449     346     335     246
                 116      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     387     487     583     893     107 
   Size:        280     100      96     310    3365

  Fragments arranged by size: 

                3365     310     280     100      96
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2101    2282    2445    2101 
   Size:        181     163    3807

  Fragments arranged by size: 

                3807     181     163
 
BtsI GCAGTG_nn'

Cuts at:    301     852    1394     301 
   Size:        551     542    3058

  Fragments arranged by size: 

                3058     551     542
 
DraI TTT'AAA

Cuts at:   1150    1150 
   Size:       4151
 
DrdI GACnn_nn'nnGTC

Cuts at:   1857    2715    1857 
   Size:        858    3293
 
EaeI y'GGCC_r

Cuts at:    422     659    1736    1910    2301    2328    3673     422 
   Size:        237    1077     174     391      27    1345     900

  Fragments arranged by size: 

                1345    1077     900     391     237     174      27
 
EagI C'GGCC_G

Cuts at:   1736    1736 
   Size:       4151
 
EarI CTCTTCn'nnn_

Cuts at:    208    1013    2174    2384     208 
   Size:        805    1161     210    1975

  Fragments arranged by size: 

                1975    1161     805     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    780    2359    2669    2815    3961     780 
   Size:       1579     310     146    1146     970

  Fragments arranged by size: 

                1579    1146     970     310     146
 
EcoICRI GAG'CTC

Cuts at:   1163    1163 
   Size:       4151
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    216     526     533     601    1976    2408    3155    3423     216 
   Size:        310       7      68    1375     432     747     268     944

  Fragments arranged by size: 

                1375     944     747     432     310     268      68       7
            
 
EcoO109I rG'GnC_Cy

Cuts at:    699    1133    1290     699 
   Size:        434     157    3560

  Fragments arranged by size: 

                3560     434     157
 
EcoRI G'AATT_C

Cuts at:   1155    1155 
   Size:       4151
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2170    2170 
   Size:       4151
 
FspI TGC'GCA

Cuts at:    592    1932     592 
   Size:       1340    2811
 
HaeII r_GCGC'y

Cuts at:    351     460     558    1585    1833    2855    3834     351 
   Size:        109      98    1027     248    1022     979     668

  Fragments arranged by size: 

                1027    1022     979     668     248     109      98
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1993    2023    2025    2055    1993 
   Size:         30       2      30    4089

  Fragments arranged by size: 

                4089      30      30       2
 
HincII GTy'rAC

Cuts at:   1190    1190 
   Size:       4151
 
KasI G'GCGC_C

Cuts at:   1581    1829    1581 
   Size:        248    3903
 
KpnI G_GTAC'C

Cuts at:   1171    1171 
   Size:       4151
 
MluI A'CGCG_T

Cuts at:   3584    3584 
   Size:       4151
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    659    2822    3006     659 
   Size:       2163     184    1804

  Fragments arranged by size: 

                2163    1804     184
 
MscI TGG'CCA

Cuts at:   1912    1912 
   Size:       4151
 
MslI CAynn'nnrTG

Cuts at:   2140    2267    2140 
   Size:        127    4024
 
MspA1I CmG'CkG

Cuts at:    309     389     453     489     585     767     924    1262    1576 
   Size:         80      64      36      96     182     157     338     314

Cuts at:   1576    1936    2949    3194     309 
   Size:        360    1013     245    1266

  Fragments arranged by size: 

                1266    1013     360     338     314     245     182     157
                  96      80      64      36
 
NaeI GCC'GGC

Cuts at:   2332    2332 
   Size:       4151
 
NarI GG'CG_CC

Cuts at:   1582    1830    1582 
   Size:        248    3903
 
NcoI C'CATG_G

Cuts at:    107     893     107 
   Size:        786    3365
 
NgoMIV G'CCGG_C

Cuts at:   2330    2330 
   Size:       4151
 
NspI r_CATG'y

Cuts at:   1204    1704    2611    1204 
   Size:        500     907    2744

  Fragments arranged by size: 

                2744     907     500
 
PasI CC'CwG_GG

Cuts at:    797     819     797 
   Size:         22    4129
 
PciI A'CATG_T

Cuts at:   2607    2607 
   Size:       4151
 
PflMI CCAn_nnn'nTGG

Cuts at:    958     984     958 
   Size:         26    4125
 
PfoI T'CCnGG_A

Cuts at:    603     603 
   Size:       4151
 
PmeI GTTT'AAAC

Cuts at:   1150    1150 
   Size:       4151
 
PmlI CAC'GTG

Cuts at:    657     657 
   Size:       4151
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3290    3322    3290 
   Size:         32    4119
 
PpuMI rG'GwC_Cy

Cuts at:    699    1133     699 
   Size:        434    3717
 
PstI C_TGCA'G

Cuts at:    308     464     563     815    1198    1883     308 
   Size:        156      99     252     383     685    2576

  Fragments arranged by size: 

                2576     685     383     252     156      99
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4151
 
PvuII CAG'CTG

Cuts at:    309     453    1576    1936     309 
   Size:        144    1123     360    2524

  Fragments arranged by size: 

                2524    1123     360     144
 
RsrII CG'GwC_CG

Cuts at:   2346    2346 
   Size:       4151
 
SacI G_AGCT'C

Cuts at:   1165    1165 
   Size:       4151
 
SacII CC_GC'GG

Cuts at:    390     490     586     390 
   Size:        100      96    3955

  Fragments arranged by size: 

                3955     100      96
 
SalI G'TCGA_C

Cuts at:   1188    1188 
   Size:       4151
 
SanDI GG'GwC_CC

Cuts at:   1133    1133 
   Size:       4151
 
SapI GCTCTTCn'nnn_

Cuts at:   2174    2384    2174 
   Size:        210    3941
 
SbfI CC_TGCA'GG

Cuts at:   1198    1198 
   Size:       4151
 
ScaI AGT'ACT

Cuts at:   3668    3668 
   Size:       4151
 
SexAI A'CCwGG_T

Cuts at:    878     878 
   Size:       4151
 
SfcI C'TryA_G

Cuts at:     33     304     460     559     811    1194    1879    2872    3063 
   Size:        271     156      99     252     383     685     993     191

Cuts at:   3063      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     685     383     271     252     191     156
                  99
 
SfoI GGC'GCC

Cuts at:   1583    1831    1583 
   Size:        248    3903
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4151
 
SmaI CCC'GGG

Cuts at:   1173    1173 
   Size:       4151
 
SmlI C'TyrA_G

Cuts at:   1306    2221    2713    2975    1306 
   Size:        915     492     262    2482

  Fragments arranged by size: 

                2482     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4151
 
SphI G_CATG'C

Cuts at:   1204    1704    1204 
   Size:        500    3651
 
StuI AGG'CCT

Cuts at:    503     787     503 
   Size:        284    3867
 
StyI C'CwwG_G

Cuts at:    107     893     957    1285    1639     107 
   Size:        786      64     328     354    2619

  Fragments arranged by size: 

                2619     786     354     328      64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1734    1867    2326    2535    1734 
   Size:        133     459     209    3350

  Fragments arranged by size: 

                3350     459     209     133
 
TatI w'GTAC_w

Cuts at:   3666    3666 
   Size:       4151
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    585     617    3668    3700     585 
   Size:         32    3051      32    1036

  Fragments arranged by size: 

                3051    1036      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1137    1948    1137 
   Size:        811    3340
 
XbaI T'CTAG_A

Cuts at:   1182    1182 
   Size:       4151
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1646    1646 
   Size:       4151
 
XmaI C'CCGG_G

Cuts at:   1171    1171 
   Size:       4151
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
    BsaXI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI    PshAI     PsiI   PspOMI    PspXI     PsrI     SfiI    SgrAI
    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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