Restriction map for pF1KB6494
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BcgI        BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        ClaI        CpoI        
DraII       DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MamI        MboII       MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NarI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfcI        SfiI        SfoI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AcsI          639  GAATTT               [GA]^AATT[CT]
AflIII        707  ACATGT               A^C[GA][CT]GT
ApoI          639  GAATTT               [GA]^AATT[CT]
AspHI         539  GAGCAC               G[AT]GC[AT]^C
BbvCI         527  CCTCAGC              CCTCAGC(-5/-2)
BciVI         715  GTATCC               GTATCC(6/5)
BclI          485  TGATCA               T^GATCA
BglII         397  AGATCT               A^GATCT
BseRI          33  CTCCTC               GAGGAG(10/8)
BsgI          296  CTGCAC               GTGCAG(16/14)
BsiHKAI       539  GAGCAC               G[AT]GC[AT]^C
BsmFI          66  GTCCC                GGGAC(10/14)
BspLU11I      707  ACATGT               A^CATGT
BsrDI         749  GCAATG               GCAATG(2/0)
BsrFI          49  GCCGGC               [GA]^CCGG[CT]
BsrI          315  CCAGT                ACTGG(1/-1)
BstAPI        132  GCACATTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI          16  TTCGAA               TT^CGAA
BstF5I        777  GGATG                GGATG(2/0)
Cfr10I         49  GCCGGC               [GA]^CCGG[CT]
Cfr13I        621  GGCCC                G^G[ACGT]CC
DraI          790  TTTAAA               TTT^AAA
DraIII        133  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          302  CCATGG               C^C[GA][CT]GG
EcoT14I       302  CCATGG               C^C[AT][AT]GG
FauI           88  CCCGC                CCCGC(4/6)
FbaI          485  TGATCA               T^GATCA
FokI          777  GGATG                GGATG(9/13)
HincII        322  GTTGAC               GT[CT]^[GA]AC
HindII        322  GTTGAC               GT[CT]^[GA]AC
HinfI          14  GATTC                G^A[ACGT]TC
HphI          464  TCACC                GGTGA(8/7)
MaeII         308  ACGT                 A^CGT
MaeIII        220  GTCAC                ^GT[ACGT]AC
MslI          222  CACTATTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI           49  GCCGGC               GCC^GGC
NcoI          302  CCATGG               C^CATGG
NdeI          371  CATATG               CA^TATG
NgoMIV         49  GCCGGC               G^CCGGC
NspV           16  TTCGAA               TT^CGAA
PflMI         601  CCAGCAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I        621  GGCCC                G^G[ACGT]CC
SexAI         629  ACCAGGT              A^CC[AT]GGT
SfaNI         802  GATGC                GCATC(5/9)
SfuI           16  TTCGAA               TT^CGAA
SmaI           69  CCCGGG               CCC^GGG
StyI          302  CCATGG               C^C[AT][AT]GG
TaqI           17  TCGA                 T^CGA
TfiI           14  GATTC                G^A[AT]TC
Tsp45I        220  GTCAC                ^GT[GC]AC
TthHB8I        17  TCGA                 T^CGA
Van91I        601  CCAGCAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          467  CCACACATATTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          69  CCCGGG               C^CCGGG
XmaI           69  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AciI           89  CCGC                 CCGC(-3/-1)
               53  GCGG                
AfaI          146  GTAC                 GT^AC
              502  GTAC                
Asp700I       456  GAATGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              588  GAATAGCTTC          
AvaI           69  CCCGGG               C^[CT]CG[GA]G
              122  CCCGAG              
BanII         119  GAGCCC               G[GA]GC[CT]^C
              125  GAGCCC              
BcnI           69  CCCGG                CC^[GC]GG
               70  CCGGG               
BpmI          362  CTCCAG               CTGGAG(16/14)
              445  CTGGAG              
BsoBI          69  CCCGGG               C^[CT]CG[GA]G
              122  CCCGAG              
BstYI         397  AGATCT               [GA]^GATC[CT]
              684  GGATCT              
HapII          50  CCGG                 C^CGG
               70  CCGG                
HpaII          50  CCGG                 C^CGG
               70  CCGG                
MflI          397  AGATCT               [GA]^GATC[CT]
              684  GGATCT              
MspI           50  CCGG                 C^CGG
               70  CCGG                
NciI           69  CCCGG                CC^[GC]GG
               70  CCGGG               
NspI          243  ACATGC               [GA]CATG^[CT]
              707  ACATGT              
RsaI          146  GTAC                 GT^AC
              502  GTAC                
SmlI           98  CTCAAG               C^T[CT][GA]AG
              688  CTCAAG              
Tsp509I       195  AATT                 ^AATT
              640  AATT                
XhoII         397  AGATCT               [GA]^GATC[CT]
              684  GGATCT              
XmnI          456  GAATGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              588  GAATAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AluI        AlwI        BbvI        BmyI        BsaJI       
BsiYI       BslI        Bsp1286I    BssKI       BstNI       
Cac8I       DdeI        DpnII       EcoRII      Fnu4HI      
HaeIII      ItaI        MboI        MnlI        MseI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       PvuII       Sau3AI      ScrFI       Tru9I       
TseI        TspRI       
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