Restriction map for pF1KE1029
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AflIII      Alw44I      ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AvaI        AviII       AvrII       BbeI        BbrPI       
BcgI        BciVI       BfrI        BlnI        BsaBI       
BsaHI       BsiEI       BsiWI       BsoBI       BspDI       
BspLU11I    BsrBI       BssHII      BstBI       BstEII      
BstPI       BstUI       ClaI        CpoI        EagI        
EclXI       Eco52I      EcoNI       EcoO65I     EcoRV       
FseI        FspI        Hin1I       HpaI        KasI        
KpnI        KspI        MamI        MluI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SfiI        SfoI        SfuI        
SmaI        SnaBI       SphI        Sse8387I    SspI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccIII       3398  TCCGGA               T^CCGGA
AgeI           30  ACCGGT               A^CCGGT
AhdI           19  GACCAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      3287  AGCGCT               AGC^GCT
ApaI          223  GGGCCC               GGGCC^C
AspEI          19  GACCAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         207  GAGCTC               G[AT]GC[AT]^C
BalI          615  TGGCCA               TGG^CCA
BanI         3726  GGTGCC               G^G[CT][GA]CC
BbvCI         727  CCTCAGC              CCTCAGC(-5/-2)
BglII        3044  AGATCT               A^GATCT
BsaAI        3301  TACGTG               [CT]AC^GT[GA]
BseAI        3398  TCCGGA               T^CCGGA
BsiHKAI       207  GAGCTC               G[AT]GC[AT]^C
BspEI        3398  TCCGGA               T^CCGGA
BspHI         449  TCATGA               T^CATGA
BssSI        1487  CTCGTG               CACGAG(-5/-1)
Bst1107I     2644  GTATAC               GTA^TAC
BstAPI         90  GCACCGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         587  CCAATGACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2644  GTATAC               GTA^TAC
Eam1105I       19  GACCAGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3287  AGCGCT               AGC^GCT
HaeII        3287  AGCGCT               [GA]GCGC^[CT]
MfeI         2078  CAATTG               C^AATTG
MluNI         615  TGGCCA               TGG^CCA
MroI         3398  TCCGGA               T^CCGGA
MscI          615  TGGCCA               TGG^CCA
MunI         2078  CAATTG               C^AATTG
NspI         3660  GCATGT               [GA]CATG^[CT]
PflMI        3689  CCAGCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI          30  ACCGGT               A^CCGGT
PpuMI        2225  AGGTCCT              [GA]G^G[AT]CC[CT]
RcaI          449  TCATGA               T^CATGA
SacI          207  GAGCTC               GAGCT^C
SapI         1417  GCTCTTC              GCTCTTC(1/4)
ScaI          164  AGTACT               AGT^ACT
SgrAI          29  CACCGGTG             C[GA]^CCGG[CT]G
SmlI         3123  CTCAAG               C^T[CT][GA]AG
SpeI         1506  ACTAGT               A^CTAGT
StuI         3632  AGGCCT               AGG^CCT
SwaI          459  ATTTAAAT             ATTT^AAAT
Van91I       3689  CCAGCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

BamHI          56  GGATCC               G^GATCC
             3395  GGATCC              
BclI         1206  TGATCA               T^GATCA
             3536  TGATCA              
BfaI         1507  CTAG                 C^TAG
             3355  CTAG                
BlpI         1492  GCTGAGC              GC^T[ACGT]AGC
             3160  GCTGAGC             
Bpu1102I     1492  GCTGAGC              GC^T[ACGT]AGC
             3160  GCTGAGC             
BsmBI        1090  CGTCTC               CGTCTC(1/5)
             2836  CGTCTC              
Bsp1407I     1581  TGTACA               T^GTACA
             1708  TGTACA              
BspMI         738  ACCTGC               ACCTGC(4/8)
             3054  GCAGGT              
BsrFI          30  ACCGGT               [GA]^CCGG[CT]
              834  GCCGGT              
BsrGI        1581  TGTACA               T^GTACA
             1708  TGTACA              
CelII        1492  GCTGAGC              GC^T[ACGT]AGC
             3160  GCTGAGC             
CfoI          376  GCGC                 GCG^C
             3288  GCGC                
Cfr10I         30  ACCGGT               [GA]^CCGG[CT]
              834  GCCGGT              
DraII        2225  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             3719  AGGGCCT             
DraIII         91  CACCGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2386  CACCATGTG           
DrdI          138  GACCATGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2218  GACACCAAGGTC        
EaeI          615  TGGCCA               [CT]^GGCC[GA]
              782  CGGCCA              
EcoO109I     2225  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             3719  AGGGCCT             
EcoT22I       815  ATGCAT               ATGCA^T
             2446  ATGCAT              
FauI          226  CCCGC                CCCGC(4/6)
             1922  CCCGC               
FbaI         1206  TGATCA               T^GATCA
             3536  TGATCA              
HgaI           51  GCGTC                GACGC(5/10)
             3612  GCGTC               
HhaI          376  GCGC                 GCG^C
             3288  GCGC                
HinP1I        376  GCGC                 G^CGC
             3288  GCGC                
HindIII      1915  AAGCTT               A^AGCTT
             3193  AAGCTT              
MaeI         1507  CTAG                 C^TAG
             3355  CTAG                
NsiI          815  ATGCAT               ATGCA^T
             2446  ATGCAT              
PstI          403  CTGCAG               CTGCA^G
             3022  CTGCAG              
SexAI         185  ACCAGGT              A^CC[AT]GGT
              392  ACCAGGT             
SspBI        1581  TGTACA               T^GTACA
             1708  TGTACA              
XcmI         1946  CCAAATTTCGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3464  CCATGGAGAATGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        AvaII       
BanII       BbsI        BbvI        BcnI        BglI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Bsu36I      Cac8I       Cfr13I      DdeI        DpnII       
DraI        DsaI        EarI        Eco81I      EcoRI       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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