Restriction map for pF1KB5623
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       AccI        AciI        AcyI        AgeI        
AhdI        AocI        Aor51HI     ApaI        AscI        
AspEI       AspI        AvaI        AviII       BbeI        
BbsI        BbvCI       BcgI        BclI        BglI        
BglII       BlpI        Bpu1102I    BpuAI       BsaHI       
BsgI        BsiWI       BsmBI       BsoBI       BspDI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       Hin1I       
HindIII     HpaI        KasI        KspI        MfeI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PshAI       PvuI        RsrII       SacII       
SalI        SapI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I       1976  GGTACC               G^GTACC
AccIII       2199  TCCGGA               T^CCGGA
AclI           83  AACGTT               AA^CGTT
Alw44I       1063  GTGCAC               G^TGCAC
ApaLI        1063  GTGCAC               G^TGCAC
AseI         2352  ATTAAT               AT^TAAT
AsnI         2352  ATTAAT               AT^TAAT
Asp700I      1077  GAATAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1976  GGTACC               G^GTACC
AvaII        1732  GGTCC                G^G[AT]CC
AvrII        2460  CCTAGG               C^CTAGG
BalI         2613  TGGCCA               TGG^CCA
BamHI         771  GGATCC               G^GATCC
BbrPI        1888  CACGTG               CAC^GTG
BcnI         2079  CCCGG                CC^[GC]GG
BlnI         2460  CCTAGG               C^CTAGG
BsaAI        1888  CACGTG               [CT]AC^GT[GA]
BsaI         2302  GAGACC               GGTCTC(1/5)
BsaWI        2199  TCCGGA               [AT]^CCGG[AT]
BseAI        2199  TCCGGA               T^CCGGA
BsiEI        2879  CGACCG               CG[GA][CT]^CG
BsmI          589  GCATTC               GAATGC(1/-1)
BspEI        2199  TCCGGA               T^CCGGA
BspHI        1049  TCATGA               T^CATGA
BspLU11I      579  ACATGT               A^CATGT
BspMI        1185  ACCTGC               ACCTGC(4/8)
BsrDI          41  CATTGC               GCAATG(2/0)
BstXI         376  CCAAATTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI         1204  GCGC                 GCG^C
EaeI         2613  TGGCCA               [CT]^GGCC[GA]
EarI          951  GAAGAG               CTCTTC(1/4)
HhaI         1204  GCGC                 GCG^C
HinP1I       1204  GCGC                 G^CGC
KpnI         1976  GGTACC               GGTAC^C
Ksp632I       951  GAAGAG               CTCTTC(1/4)
MluI          130  ACGCGT               A^CGCGT
MluNI        2613  TGGCCA               TGG^CCA
MroI         2199  TCCGGA               T^CCGGA
MscI         2613  TGGCCA               TGG^CCA
MspA1I       2726  CAGCTG               C[AC]G^C[GT]G
NciI         2079  CCCGG                CC^[GC]GG
PmaCI        1888  CACGTG               CAC^GTG
PmlI         1888  CACGTG               CAC^GTG
PpuMI        1731  AGGTCCC              [GA]G^G[AT]CC[CT]
PshBI        2352  ATTAAT               AT^TAAT
Psp1406I       83  AACGTT               AA^CGTT
PstI         1568  CTGCAG               CTGCA^G
PvuII        2726  CAGCTG               CAG^CTG
RcaI         1049  TCATGA               T^CATGA
SfcI         1568  CTGCAG               C^T[GA][CT]AG
SpeI         2152  ACTAGT               A^CTAGT
SphI         1377  GCATGC               GCATG^C
XmnI         1077  GAATAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII         131  CGCG                 CG^CG
             1205  CGCG                
AflII         945  CTTAAG               C^TTAAG
             1170  CTTAAG              
AflIII        130  ACGCGT               A^C[GA][CT]GT
              579  ACATGT              
BfrI          945  CTTAAG               C^TTAAG
             1170  CTTAAG              
BsaBI         204  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2223  GATTCATATC          
Bsp1407I      417  TGTACA               T^GTACA
              862  TGTACA              
BsrGI         417  TGTACA               T^GTACA
              862  TGTACA              
BstUI         131  CGCG                 CG^CG
             1205  CGCG                
DsaI         1110  CCATGG               C^C[GA][CT]GG
             2240  CCATGG              
HapII        2080  CCGG                 C^CGG
             2200  CCGG                
HincII       1015  GTCAAC               GT[CT]^[GA]AC
             2493  GTTGAC              
HindII       1015  GTCAAC               GT[CT]^[GA]AC
             2493  GTTGAC              
HpaII        2080  CCGG                 C^CGG
             2200  CCGG                
MamI          204  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2223  GATTCATATC          
MspI         2080  CCGG                 C^CGG
             2200  CCGG                
MvnI          131  CGCG                 CG^CG
             1205  CGCG                
NcoI         1110  CCATGG               C^CATGG
             2240  CCATGG              
NspI          579  ACATGT               [GA]CATG^[CT]
             1377  GCATGC              
SacI          304  GAGCTC               GAGCT^C
             1444  GAGCTC              
ScaI          705  AGTACT               AGT^ACT
             1163  AGTACT              
SexAI         275  ACCAGGT              A^CC[AT]GGT
             1959  ACCTGGT             
SspBI         417  TGTACA               T^GTACA
              862  TGTACA              
XcmI         1190  CCACAGAGTACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1523  CCAACAACAGTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        AlwNI       
ApoI        AspHI       BanI        BanII       BbvI        
BciVI       BfaI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HgaI        HinfI       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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