Restriction map for pF1KB4741
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

Acc65I      AccI        AccIII      AflII       AgeI        
AhdI        AlwNI       ApaI        AscI        Asp718I     
AspEI       AspI        AviII       AvrII       BbeI        
BbrPI       BbvCI       BciVI       BcnI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrFI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoNI       EcoO65I     
EcoRV       FseI        FspI        HapII       HindIII     
HpaI        HpaII       KasI        KpnI        MamI        
MfeI        MluI        MroI        MspI        MunI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PvuI        RcaI        
RsrII       SacI        SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SwaI        Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII        3184  GACGTC               GACGT^C
AccII        1499  CGCG                 CG^CG
AclI         1064  AACGTT               AA^CGTT
AcyI         3184  GACGTC               G[GA]^CG[CT]C
AflIII        196  ACATGT               A^C[GA][CT]GT
Alw44I       2068  GTGCAC               G^TGCAC
AocI         1990  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       148  AGCGCT               AGC^GCT
ApaLI        2068  GTGCAC               G^TGCAC
AvaI         1835  CTCGGG               C^[CT]CG[GA]G
BalI         1972  TGGCCA               TGG^CCA
BanII         135  GAGCCC               G[GA]GC[CT]^C
BbsI         1466  GAAGAC               GAAGAC(2/6)
BcgI         2185  CGACAGAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          839  TGATCA               T^GATCA
BglII        1143  AGATCT               A^GATCT
BpuAI        1466  GAAGAC               GAAGAC(2/6)
BsaHI        3184  GACGTC               G[GA]^CG[CT]C
BsmBI        2339  CGTCTC               CGTCTC(1/5)
BsmI         2031  GAATGC               GAATGC(1/-1)
BsoBI        1835  CTCGGG               C^[CT]CG[GA]G
Bsp1407I      448  TGTACA               T^GTACA
BspLU11I      196  ACATGT               A^CATGT
BsrGI         448  TGTACA               T^GTACA
BssSI        3366  CACGAG               CACGAG(-5/-1)
BstUI        1499  CGCG                 CG^CG
Bsu36I       1990  CCTGAGG              CC^T[ACGT]AGG
DraII        1695  AGGTCCC              [GA]G^G[ACGT]CC[CT]
Eco47III      148  AGCGCT               AGC^GCT
Eco81I       1990  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     1695  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I      2918  CCAAGG               C^C[AT][AT]GG
FbaI          839  TGATCA               T^GATCA
HaeII         148  AGCGCT               [GA]GCGC^[CT]
HgaI          352  GCGTC                GACGC(5/10)
Hin1I        3184  GACGTC               G[GA]^CG[CT]C
KspI         1498  CCGCGG               CCGC^GG
MluNI        1972  TGGCCA               TGG^CCA
MscI         1972  TGGCCA               TGG^CCA
MvnI         1499  CGCG                 CG^CG
PpuMI        1695  AGGTCCC              [GA]G^G[AT]CC[CT]
Psp1406I     1064  AACGTT               AA^CGTT
PvuII        1968  CAGCTG               CAG^CTG
SacII        1498  CCGCGG               CCGC^GG
ScaI         2738  AGTACT               AGT^ACT
SspBI         448  TGTACA               T^GTACA
StyI         2918  CCAAGG               C^C[AT][AT]GG
XcmI          970  CCAAGCCAGGTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AseI          399  ATTAAT               AT^TAAT
             2925  ATTAAT              
AsnI          399  ATTAAT               AT^TAAT
             2925  ATTAAT              
Asp700I       427  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2031  GAATGCCTTC          
BamHI        1995  GGATCC               G^GATCC
             2322  GGATCC              
BanI         1986  GGTGCC               G^G[CT][GA]CC
             3313  GGTGCC              
BsaI         2638  GAGACC               GGTCTC(1/5)
             2717  GAGACC              
BseRI         965  CTCCTC               GAGGAG(10/8)
             2008  CTCCTC              
CfoI          149  GCGC                 GCG^C
             2091  GCGC                
DsaI         1498  CCGCGG               C^C[GA][CT]GG
             1980  CCACGG              
EaeI         1813  TGGCCG               [CT]^GGCC[GA]
             1972  TGGCCA              
EcoT22I      1018  ATGCAT               ATGCA^T
             2573  ATGCAT              
FauI         1497  CCCGC                CCCGC(4/6)
             1500  GCGGG               
HhaI          149  GCGC                 GCG^C
             2091  GCGC                
HinP1I        149  GCGC                 G^CGC
             2091  GCGC                
HincII       1567  GTTGAC               GT[CT]^[GA]AC
             2779  GTTGAC              
HindII       1567  GTTGAC               GT[CT]^[GA]AC
             2779  GTTGAC              
MspA1I       1498  CCGCGG               C[AC]G^C[GT]G
             1968  CAGCTG              
NdeI         2453  CATATG               CA^TATG
             2762  CATATG              
NsiI         1018  ATGCAT               ATGCA^T
             2573  ATGCAT              
PshBI         399  ATTAAT               AT^TAAT
             2925  ATTAAT              
XmnI          427  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2031  GAATGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BbvI        BfaI        
BmyI        BpmI        BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        DraIII      EarI        EcoRI       EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspI        StuI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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