Restriction map for pF1KB4741
 (Linear) (Six-base) MAPSORT of: KIBB4741.seq  Check: 2326  from: 1  to: 3435



KIBB4741.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AatII G_ACGT'C

Cuts at:      0    3188    3435 
   Size:       3188     247
 
AclI AA'CG_TT

Cuts at:      0    1065    3435 
   Size:       1065    2370
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2022    2034    2624    2653    2999    3353    3371    3435 
   Size:       2022      12     590      29     346     354      18      64

  Fragments arranged by size: 

                2022     590     354     346      64      29      18      12
            
 
AfeI AGC'GCT

Cuts at:      0     150    3435 
   Size:        150    3285
 
AflIII A'CryG_T

Cuts at:      0     196    3435 
   Size:        196    3239
 
AleI CACnn'nnGTG

Cuts at:      0    2075    3435 
   Size:       2075    1360
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2093    2924    3435 
   Size:       2093     831     511

  Fragments arranged by size: 

                2093     831     511
 
ApaLI G'TGCA_C

Cuts at:      0    2068    3435 
   Size:       2068    1367
 
ApoI r'AATT_y

Cuts at:      0     858    1483    2277    2356    2436    2970    3256    3435 
   Size:        858     625     794      79      80     534     286     179

  Fragments arranged by size: 

                 858     794     625     534     286     179      80      79
            
 
AseI AT'TA_AT

Cuts at:      0     400    2926    3435 
   Size:        400    2526     509

  Fragments arranged by size: 

                2526     509     400
 
AvaI C'yCGr_G

Cuts at:      0    1835    3435 
   Size:       1835    1600
 
BamHI G'GATC_C

Cuts at:      0    1995    2322    3435 
   Size:       1995     327    1113

  Fragments arranged by size: 

                1995    1113     327
 
BanI G'GyrC_C

Cuts at:      0    1986    3313    3435 
   Size:       1986    1327     122

  Fragments arranged by size: 

                1986    1327     122
 
BanII G_rGCy'C

Cuts at:      0     139    3435 
   Size:        139    3296
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1473    3435 
   Size:       1473    1962
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2174    2208    3435 
   Size:       2174      34    1227

  Fragments arranged by size: 

                2174    1227      34
 
BclI T'GATC_A

Cuts at:      0     839    3435 
   Size:        839    2596
 
BfrBI ATG'CAT

Cuts at:      0    1020    2575    3435 
   Size:       1020    1555     860

  Fragments arranged by size: 

                1555    1020     860
 
BglII A'GATC_T

Cuts at:      0    1143    3435 
   Size:       1143    2292
 
Bme1580I G_kGCm'C

Cuts at:      0    1834    2072    3318    3435 
   Size:       1834     238    1246     117

  Fragments arranged by size: 

                1834    1246     238     117
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2211    2324    2751    3435 
   Size:       2211     113     427     684

  Fragments arranged by size: 

                2211     684     427     113
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3023    3435 
   Size:       3023     412
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1223    1746    2406    2910    3435 
   Size:       1223     523     660     504     525

  Fragments arranged by size: 

                1223     660     525     523     504
 
Bpu10I CC'TnA_GC

Cuts at:      0    2179    2621    3435 
   Size:       2179     442     814

  Fragments arranged by size: 

                2179     814     442
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2632    2711    3435 
   Size:       2632      79     724

  Fragments arranged by size: 

                2632     724      79
 
BsaHI Gr'CG_yC

Cuts at:      0    3185    3435 
   Size:       3185     250
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1838    1868    3435 
   Size:       1838      30    1567

  Fragments arranged by size: 

                1838    1567      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     956    1999    3435 
   Size:        956    1043    1436

  Fragments arranged by size: 

                1436    1043     956
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     713    1673    1823    3435 
   Size:        713     960     150    1612

  Fragments arranged by size: 

                1612     960     713     150
 
BsiHKAI G_wGCw'C

Cuts at:      0     966    2072    2736    3435 
   Size:        966    1106     664     699

  Fragments arranged by size: 

                1106     966     699     664
 
BsmI GAATG_Cn'

Cuts at:      0    2037    3435 
   Size:       2037    1398
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2345    3435 
   Size:       2345    1090
 
Bsp1286I G_dGCh'C

Cuts at:      0     139     966    1834    2072    2736    3318    3435 
   Size:        139     827     868     238     664     582     117

  Fragments arranged by size: 

                 868     827     664     582     238     139     117
 
BsrDI GCAATG_nn'

Cuts at:      0     905     944    1682    3188    3435 
   Size:        905      39     738    1506     247

  Fragments arranged by size: 

                1506     905     738     247      39
 
BsrGI T'GTAC_A

Cuts at:      0     448    3435 
   Size:        448    2987
 
BssSI C'ACGA_G

Cuts at:      0    3366    3435 
   Size:       3366      69
 
BstYI r'GATC_y

Cuts at:      0    1143    1339    1995    2322    3031    3435 
   Size:       1143     196     656     327     709     404

  Fragments arranged by size: 

                1143     709     656     404     327     196
 
Bsu36I CC'TnA_GG

Cuts at:      0    1991    3435 
   Size:       1991    1444
 
BtgI C'CryG_G

Cuts at:      0    1498    1980    3435 
   Size:       1498     482    1455

  Fragments arranged by size: 

                1498    1455     482
 
BtsI GCAGTG_nn'

Cuts at:      0    2070    2082    3018    3435 
   Size:       2070      12     936     417

  Fragments arranged by size: 

                2070     936     417      12
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1740    1775    3435 
   Size:       1740      35    1660

  Fragments arranged by size: 

                1740    1660      35
 
DraI TTT'AAA

Cuts at:      0     485     863    3422    3435 
   Size:        485     378    2559      13

  Fragments arranged by size: 

                2559     485     378      13
 
DraIII CAC_nnn'GTG

Cuts at:      0    1986    2332    3313    3435 
   Size:       1986     346     981     122

  Fragments arranged by size: 

                1986     981     346     122
 
EaeI y'GGCC_r

Cuts at:      0    1813    1972    3435 
   Size:       1813     159    1463

  Fragments arranged by size: 

                1813    1463     159
 
EarI CTCTTCn'nnn_

Cuts at:      0    1820    1935    3086    3435 
   Size:       1820     115    1151     349

  Fragments arranged by size: 

                1820    1151     349     115
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1223    1746    2022    2034    2406    2624    2653    2910 
   Size:       1223     523     276      12     372     218      29     257

Cuts at:   2910    2999    3353    3371    3435 
   Size:         89     354      18      64

  Fragments arranged by size: 

                1223     523     372     354     276     257     218      89
                  64      29      18      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1696    3435 
   Size:       1696    1739
 
EcoRI G'AATT_C

Cuts at:      0    2356    2436    2970    3435 
   Size:       2356      80     534     465

  Fragments arranged by size: 

                2356     534     465      80
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1486    3434    3435 
   Size:       1486    1948       1

  Fragments arranged by size: 

                1948    1486       1
 
HaeII r_GCGC'y

Cuts at:      0     152    3435 
   Size:        152    3283
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1612    1644    2512    2544    2604    2636    3435 
   Size:       1612      32     868      32      60      32     799

  Fragments arranged by size: 

                1612     868     799      60      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1569    2781    3435 
   Size:       1569    1212     654

  Fragments arranged by size: 

                1569    1212     654
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1412    3435 
   Size:       1412    2023
 
MscI TGG'CCA

Cuts at:      0    1974    3435 
   Size:       1974    1461
 
MslI CAynn'nnrTG

Cuts at:      0     223    2065    2075    3435 
   Size:        223    1842      10    1360

  Fragments arranged by size: 

                1842    1360     223      10
 
MspA1I CmG'CkG

Cuts at:      0    1500    1970    3435 
   Size:       1500     470    1465

  Fragments arranged by size: 

                1500    1465     470
 
NdeI CA'TA_TG

Cuts at:      0    2454    2763    3435 
   Size:       2454     309     672

  Fragments arranged by size: 

                2454     672     309
 
NsiI A_TGCA'T

Cuts at:      0    1022    2577    3435 
   Size:       1022    1555     858

  Fragments arranged by size: 

                1555    1022     858
 
NspI r_CATG'y

Cuts at:      0     200     898    1024    3435 
   Size:        200     698     126    2411

  Fragments arranged by size: 

                2411     698     200     126
 
PasI CC'CwG_GG

Cuts at:      0    1040    1700    3435 
   Size:       1040     660    1735

  Fragments arranged by size: 

                1735    1040     660
 
PciI A'CATG_T

Cuts at:      0     196    3435 
   Size:        196    3239
 
PfoI T'CCnGG_A

Cuts at:      0     690    1200    3435 
   Size:        690     510    2235

  Fragments arranged by size: 

                2235     690     510
 
PpuMI rG'GwC_Cy

Cuts at:      0    1696    3435 
   Size:       1696    1739
 
PsiI TTA'TAA

Cuts at:      0    1238    1442    1589    2584    3435 
   Size:       1238     204     147     995     851

  Fragments arranged by size: 

                1238     995     851     204     147
 
PstI C_TGCA'G

Cuts at:      0    2077    2913    3025    3435 
   Size:       2077     836     112     410

  Fragments arranged by size: 

                2077     836     410     112
 
PvuII CAG'CTG

Cuts at:      0    1970    3435 
   Size:       1970    1465
 
SacII CC_GC'GG

Cuts at:      0    1501    3435 
   Size:       1501    1934
 
ScaI AGT'ACT

Cuts at:      0    2740    3435 
   Size:       2740     695
 
SfcI C'TryA_G

Cuts at:      0     534    2073    2909    3021    3435 
   Size:        534    1539     836     112     414

  Fragments arranged by size: 

                1539     836     534     414     112
 
SspI AAT'ATT

Cuts at:      0     404     498     528    1153    3050    3435 
   Size:        404      94      30     625    1897     385

  Fragments arranged by size: 

                1897     625     404     385      94      30
 
StuI AGG'CCT

Cuts at:      0    1035    1177    2132    3435 
   Size:       1035     142     955    1303

  Fragments arranged by size: 

                1303    1035     955     142
 
StyI C'CwwG_G

Cuts at:      0    2918    3435 
   Size:       2918     517
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2343    3435 
   Size:       2343    1092
 
TatI w'GTAC_w

Cuts at:      0     379     448    2219    2738    3001    3435 
   Size:        379      69    1771     519     263     434

  Fragments arranged by size: 

                1771     519     434     379     263      69
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1785    1817    1931    1963    3435 
   Size:       1785      32     114      32    1472

  Fragments arranged by size: 

                1785    1472     114      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     977    3435 
   Size:        977    2458
 
XmnI GAAnn'nnTTC

Cuts at:      0     431    2035    3435 
   Size:        431    1604    1400

  Fragments arranged by size: 

                1604    1400     431
 
ZraI GAC'GTC

Cuts at:      0    3186    3435 
   Size:       3186     249
 

 Enzymes that do cut:

    AatII     AclI     AcuI     AfeI   AflIII     AleI     AlfI    ApaLI
     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
     BclI    BfrBI    BglII Bme1580I     BmrI     BplI     BpmI   Bpu10I
     BsaI    BsaHI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BsrDI    BsrGI    BssSI    BstYI   Bsu36I     BtgI     BtsI
    CspCI     DraI   DraIII     EaeI     EarI  Eco57MI EcoO109I    EcoRI
     FalI    HaeII    Hin4I   HincII     MmeI     MscI     MslI   MspA1I
     NdeI     NsiI     NspI     PasI     PciI     PfoI    PpuMI     PsiI
     PstI    PvuII    SacII     ScaI     SfcI     SspI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I    AflII     AgeI     AhdI     AloI    AlwNI
     ApaI     AscI    AsiSI    AvrII     BaeI     BbeI    BbvCI    BciVI
     BglI     BlpI    BmgBI     BmtI    BpuEI    BsaAI    BsaBI    BsaWI
    BseYI    BsiEI    BsiWI    BspEI    BspHI    BspMI    BsrBI    BsrFI
   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI    PflMI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SwaI  Tth111I     XbaI     XhoI     XmaI
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