Restriction map for pF1KB3472
 (Linear) (Six-base) MAPSORT of: KIBB3472.seq  Check: 1962  from: 1  to: 2349



KIBB3472.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AccI GT'mk_AC

Cuts at:      0    1871    2349 
   Size:       1871     478
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     215     469     602     822    1115    2154    2184 
   Size:        143      72     254     133     220     293    1039      30

Cuts at:   2184    2349 
   Size:        165

  Fragments arranged by size: 

                1039     293     254     220     165     143     133      72
                  30
 
AfeI AGC'GCT

Cuts at:      0    1879    2349 
   Size:       1879     470
 
AflII C'TTAA_G

Cuts at:      0     997    2349 
   Size:        997    1352
 
AlwNI CAG_nnn'CTG

Cuts at:      0     700     789    1359    2349 
   Size:        700      89     570     990

  Fragments arranged by size: 

                 990     700     570      89
 
ApoI r'AATT_y

Cuts at:      0     207    1368    2349 
   Size:        207    1161     981

  Fragments arranged by size: 

                1161     981     207
 
AvaI C'yCGr_G

Cuts at:      0    2259    2349 
   Size:       2259      90
 
BanI G'GyrC_C

Cuts at:      0    1858    2349 
   Size:       1858     491
 
BanII G_rGCy'C

Cuts at:      0     302    2349 
   Size:        302    2047
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     712    1103    2349 
   Size:        712     391    1246

  Fragments arranged by size: 

                1246     712     391
 
BclI T'GATC_A

Cuts at:      0    1050    1886    2349 
   Size:       1050     836     463

  Fragments arranged by size: 

                1050     836     463
 
BglII A'GATC_T

Cuts at:      0     984    2349 
   Size:        984    1365
 
Bme1580I G_kGCm'C

Cuts at:      0     780    2349 
   Size:        780    1569
 
BmgBI CAC'GTC

Cuts at:      0    1865    2349 
   Size:       1865     484
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     482     710    2349 
   Size:        482     228    1639

  Fragments arranged by size: 

                1639     482     228
 
Bpu10I CC'TnA_GC

Cuts at:      0     383    2349 
   Size:        383    1966
 
BsaAI yAC'GTr

Cuts at:      0    1495    2349 
   Size:       1495     854
 
BsaBI GATnn'nnATC

Cuts at:      0     928    2349 
   Size:        928    1421
 
BsaWI w'CCGG_w

Cuts at:      0     474    2349 
   Size:        474    1875
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1457    1487    1864    1894    2316    2346    2349 
   Size:       1457      30     377      30     422      30       3

  Fragments arranged by size: 

                1457     422     377      30      30      30       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      97     256     367     626    1822    2002    2349 
   Size:         97     159     111     259    1196     180     347

  Fragments arranged by size: 

                1196     347     259     180     159     111      97
 
BseYI C'CCAG_C

Cuts at:      0    1980    2034    2349 
   Size:       1980      54     315

  Fragments arranged by size: 

                1980     315      54
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1475    2349 
   Size:       1475     874
 
BsiHKAI G_wGCw'C

Cuts at:      0    1930    2349 
   Size:       1930     419
 
Bsp1286I G_dGCh'C

Cuts at:      0     302     780    1930    2349 
   Size:        302     478    1150     419

  Fragments arranged by size: 

                1150     478     419     302
 
BsrDI GCAATG_nn'

Cuts at:      0      53    1111    2349 
   Size:         53    1058    1238

  Fragments arranged by size: 

                1238    1058      53
 
BstYI r'GATC_y

Cuts at:      0     984    2299    2349 
   Size:        984    1315      50

  Fragments arranged by size: 

                1315     984      50
 
Bsu36I CC'TnA_GG

Cuts at:      0     782    1511    2349 
   Size:        782     729     838

  Fragments arranged by size: 

                 838     782     729
 
BtgI C'CryG_G

Cuts at:      0    2149    2349 
   Size:       2149     200
 
BtsI GCAGTG_nn'

Cuts at:      0      64    1763    1930    2349 
   Size:         64    1699     167     419

  Fragments arranged by size: 

                1699     419     167      64
 
ClaI AT'CG_AT

Cuts at:      0    1569    2349 
   Size:       1569     780
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1211    1246    2349 
   Size:       1211      35    1103

  Fragments arranged by size: 

                1211    1103      35
 
EaeI y'GGCC_r

Cuts at:      0    2120    2349 
   Size:       2120     229
 
EarI CTCTTCn'nnn_

Cuts at:      0     119     131     760    1055    2349 
   Size:        119      12     629     295    1294

  Fragments arranged by size: 

                1294     629     295     119      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1593    2349 
   Size:       1593     756
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     215     469     602     822    1115    2154    2184 
   Size:        143      72     254     133     220     293    1039      30

Cuts at:   2184    2349 
   Size:        165

  Fragments arranged by size: 

                1039     293     254     220     165     143     133      72
                  30
 
EcoRI G'AATT_C

Cuts at:      0     207    1368    2349 
   Size:        207    1161     981

  Fragments arranged by size: 

                1161     981     207
 
EcoRV GAT'ATC

Cuts at:      0     930    2349 
   Size:        930    1419
 
HaeII r_GCGC'y

Cuts at:      0     560    1881    2349 
   Size:        560    1321     468

  Fragments arranged by size: 

                1321     560     468
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     232     264     636     668    1307    1339    1378    1410 
   Size:        232      32     372      32     639      32      39      32

Cuts at:   1410    1540    1572    1864    1896    2349 
   Size:        130      32     292      32     453

  Fragments arranged by size: 

                 639     453     372     292     232     130      39      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     201    2349 
   Size:        201    2148
 
HindIII A'AGCT_T

Cuts at:      0    1718    2349 
   Size:       1718     631
 
MfeI C'AATT_G

Cuts at:      0    1931    2349 
   Size:       1931     418
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     918    1013    2349 
   Size:        918      95    1336

  Fragments arranged by size: 

                1336     918      95
 
MscI TGG'CCA

Cuts at:      0    2122    2349 
   Size:       2122     227
 
MslI CAynn'nnrTG

Cuts at:      0      46    2349 
   Size:         46    2303
 
MspA1I CmG'CkG

Cuts at:      0     665    1792    1980    2181    2211    2241    2349 
   Size:        665    1127     188     201      30      30     108

  Fragments arranged by size: 

                1127     665     201     188     108      30      30
 
NdeI CA'TA_TG

Cuts at:      0    1179    2349 
   Size:       1179    1170
 
NspI r_CATG'y

Cuts at:      0    1006    2349 
   Size:       1006    1343
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1032    2349 
   Size:       1032    1317
 
PfoI T'CCnGG_A

Cuts at:      0    1010    2349 
   Size:       1010    1339
 
PmlI CAC'GTG

Cuts at:      0    1495    2349 
   Size:       1495     854
 
PvuII CAG'CTG

Cuts at:      0    1792    1980    2349 
   Size:       1792     188     369

  Fragments arranged by size: 

                1792     369     188
 
SexAI A'CCwGG_T

Cuts at:      0     945    2349 
   Size:        945    1404
 
SmlI C'TyrA_G

Cuts at:      0     997    2349 
   Size:        997    1352
 
SspI AAT'ATT

Cuts at:      0    1947    2349 
   Size:       1947     402
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2026    2349 
   Size:       2026     323
 
TatI w'GTAC_w

Cuts at:      0    1647    2349 
   Size:       1647     702
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1457    1489    2349 
   Size:       1457      32     860

  Fragments arranged by size: 

                1457     860      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     970    1743    2349 
   Size:        970     773     606

  Fragments arranged by size: 

                 970     773     606
 
XmnI GAAnn'nnTTC

Cuts at:      0     709    2349 
   Size:        709    1640
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AflII    AlwNI     ApoI     AvaI     BanI
    BanII     BbsI     BclI    BglII Bme1580I    BmgBI     BmrI   Bpu10I
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BsrDI    BstYI   Bsu36I     BtgI     BtsI     ClaI    CspCI
     EaeI     EarI     EciI  Eco57MI    EcoRI    EcoRV    HaeII    Hin4I
   HincII  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NdeI
     NspI    PflMI     PfoI     PmlI    PvuII    SexAI     SmlI     SspI
    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI
     BlpI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaHI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I
     FalI     FseI     FspI    FspAI     HpaI     KasI     KpnI     MluI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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