Restriction map for pF1KE0298
##################################
# NO CUT ENZYMES (   51 enzymes) #
##################################

AatII       AccIII      AgeI        AhdI        Aor51HI     
AscI        AspEI       AviII       AvrII       BlnI        
BsaWI       BseAI       BsiEI       BsiWI       BspEI       
BspHI       BsrFI       BssHII      Bst1107I    BstZ17I     
Cfr10I      CpoI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       FseI        FspI        
HpaI        MluI        MroI        NaeI        NgoMIV      
NotI        NruI        PacI        PinAI       PmeI        
PshAI       PvuI        RcaI        RsrII       SfiI        
SgrAI       SmaI        SnaBI       SwaI        XmaCI       
XmaI        


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# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I       7218  GGTACC               G^GTACC
AclI         4504  AACGTT               AA^CGTT
ApaI         3294  GGGCCC               GGGCC^C
Asp718I      7218  GGTACC               G^GTACC
AspI         7788  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         2216  TGGCCA               TGG^CCA
BbrPI        7927  CACGTG               CAC^GTG
BbvCI        4399  CCTCAGC              CCTCAGC(-5/-2)
BciVI        6471  GGATAC               GTATCC(6/5)
BclI         3756  TGATCA               T^GATCA
BglI         7606  GCCCCTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         7012  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     7012  GCTGAGC              GC^T[ACGT]AGC
BsmBI        6444  CGTCTC               CGTCTC(1/5)
BspDI        4006  ATCGAT               AT^CGAT
BssSI        1429  CACGAG               CACGAG(-5/-1)
BstBI        5612  TTCGAA               TT^CGAA
BstXI        2778  CCATGCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        7012  GCTGAGC              GC^T[ACGT]AGC
ClaI         4006  ATCGAT               AT^CGAT
DraIII        644  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I       534  ATGCAT               ATGCA^T
FbaI         3756  TGATCA               T^GATCA
KpnI         7218  GGTACC               GGTAC^C
MfeI         4681  CAATTG               C^AATTG
MluNI        2216  TGGCCA               TGG^CCA
MscI         2216  TGGCCA               TGG^CCA
MunI         4681  CAATTG               C^AATTG
NdeI          793  CATATG               CA^TATG
NheI         6631  GCTAGC               G^CTAGC
NsiI          534  ATGCAT               ATGCA^T
NspV         5612  TTCGAA               TT^CGAA
PaeR7I       4232  CTCGAG               C^TCGAG
PflFI        7788  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        7927  CACGTG               CAC^GTG
PmlI         7927  CACGTG               CAC^GTG
Psp1406I     4504  AACGTT               AA^CGTT
SbfI         7858  CCTGCAGG             CCTGCA^GG
ScaI         6679  AGTACT               AGT^ACT
SfuI         5612  TTCGAA               TT^CGAA
SphI         7732  GCATGC               GCATG^C
Sse8387I     7858  CCTGCAGG             CCTGCA^GG
Tth111I      7788  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         6652  TCTAGA               T^CTAGA
XcmI         7505  CCAATACAAATATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         4232  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AflIII       1820  ACATGT               A^C[GA][CT]GT
             3651  ACATGT              
AseI         4111  ATTAAT               AT^TAAT
             4812  ATTAAT              
AsnI         4111  ATTAAT               AT^TAAT
             4812  ATTAAT              
AvaI         3560  CTCGGG               C^[CT]CG[GA]G
             4232  CTCGAG              
BbeI         1495  GGCGCC               GGCGC^C
             1525  GGCGCC              
BcgI          149  GCAAAAAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4154  GCAAATCATTCG        
BsaAI        1912  CACGTA               [CT]AC^GT[GA]
             7927  CACGTG              
BsoBI        3560  CTCGGG               C^[CT]CG[GA]G
             4232  CTCGAG              
Bsp1407I     4335  TGTACA               T^GTACA
             5662  TGTACA              
BspLU11I     1820  ACATGT               A^CATGT
             3651  ACATGT              
BsrBI        1275  CCGCTC               CCGCTC(-3/-3)
             2236  GAGCGG              
BsrGI        4335  TGTACA               T^GTACA
             5662  TGTACA              
DrdI         6031  GACTTATTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             6685  GACGAGGATGTC        
EaeI         2216  TGGCCA               [CT]^GGCC[GA]
             7407  CGGCCA              
EcoRI        1860  GAATTC               G^AATTC
             6109  GAATTC              
EcoRV        2241  GATATC               GAT^ATC
             4531  GATATC              
KasI         1495  GGCGCC               G^GCGCC
             1525  GGCGCC              
KspI         2305  CCGCGG               CCGC^GG
             8080  CCGCGG              
NarI         1495  GGCGCC               GG^CGCC
             1525  GGCGCC              
PshBI        4111  ATTAAT               AT^TAAT
             4812  ATTAAT              
SacI         4855  GAGCTC               GAGCT^C
             7700  GAGCTC              
SacII        2305  CCGCGG               CCGC^GG
             8080  CCGCGG              
SalI         3324  GTCGAC               G^TCGAC
             5195  GTCGAC              
SapI         4363  GCTCTTC              GCTCTTC(1/4)
             4718  GCTCTTC             
SexAI        2193  ACCAGGT              A^CC[AT]GGT
             7775  ACCAGGT             
SfoI         1495  GGCGCC               GGC^GCC
             1525  GGCGCC              
SpeI         4408  ACTAGT               A^CTAGT
             4974  ACTAGT              
SspBI        4335  TGTACA               T^GTACA
             5662  TGTACA              
SspI         6195  AATATT               AAT^ATT
             7489  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (  132 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflII       AluI        Alw44I      AlwI        
AlwNI       AocI        ApaLI       ApoI        Asp700I     
AspHI       AvaII       BamHI       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BfrI        
BglII       BmyI        BpmI        BpuAI       BsaBI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BspMI       BsrDI       BsrI        
BssKI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EarI        Eco81I      EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmnI        
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