Restriction map for pF1KE0100
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       Acc65I      AccIII      AflII       AgeI        
AhdI        Alw44I      AocI        ApaLI       AscI        
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BalI        BbvCI       BcgI        BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaWI       BseAI       BsiHKAI     BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        DraII       DrdI        
Eam1105I    Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        HincII      HindII      HindIII     HpaI        
KpnI        MamI        MluI        MluNI       MroI        
MscI        NaeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PpuMI       PshAI       
PvuI        RcaI        SacI        SalI        SbfI        
ScaI        SexAI       SfiI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AclI         1558  AACGTT               AA^CGTT
AcyI          148  GGCGCC               G[GA]^CG[CT]C
AflIII       2345  ACGTGT               A^C[GA][CT]GT
AlwNI         170  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      2230  AGCGCT               AGC^GCT
ApaI           61  GGGCCC               GGGCC^C
BamHI        1958  GGATCC               G^GATCC
BbeI          148  GGCGCC               GGCGC^C
BbrPI        1489  CACGTG               CAC^GTG
BsaHI         148  GGCGCC               G[GA]^CG[CT]C
BsgI          685  GTGCAG               GTGCAG(16/14)
BsiEI         188  CGGCCG               CG[GA][CT]^CG
BsmFI        1174  GGGAC                GGGAC(10/14)
BsrBI         251  CCGCTC               CCGCTC(-3/-3)
BsrI          270  ACTGG                ACTGG(1/-1)
BstAPI        169  GCAGTGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2042  TTCGAA               TT^CGAA
CpoI          156  CGGTCCG              CG^G[AT]CCG
DraI         2071  TTTAAA               TTT^AAA
DraIII        846  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          188  CGGCCG               C^GGCCG
EclXI         188  CGGCCG               C^GGCCG
Eco47III     2230  AGCGCT               AGC^GCT
Eco52I        188  CGGCCG               C^GGCCG
EcoNI        2205  CCTCCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I      2300  CCATGG               C^C[AT][AT]GG
Hin1I         148  GGCGCC               G[GA]^CG[CT]C
KasI          148  GGCGCC               G^GCGCC
KspI          256  CCGCGG               CCGC^GG
NarI          148  GGCGCC               GG^CGCC
NcoI         2300  CCATGG               C^CATGG
NdeI         1820  CATATG               CA^TATG
NspV         2042  TTCGAA               TT^CGAA
PacI         1642  TTAATTAA             TTAAT^TAA
PmaCI        1489  CACGTG               CAC^GTG
PmlI         1489  CACGTG               CAC^GTG
Psp1406I     1558  AACGTT               AA^CGTT
PvuII        1573  CAGCTG               CAG^CTG
RsrII         156  CGGTCCG              CG^G[AT]CCG
SacII         256  CCGCGG               CCGC^GG
SfoI          148  GGCGCC               GGC^GCC
SfuI         2042  TTCGAA               TT^CGAA
SmaI           64  CCCGGG               CCC^GGG
StyI         2300  CCATGG               C^C[AT][AT]GG
Tsp45I        701  GTCAC                ^GT[GC]AC
XmaCI          64  CCCGGG               C^CCGGG
XmaI           64  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI          890  GTCTAC               GT^[AC][GT]AC
             1374  GTCTAC              
AccII         102  CGCG                 CG^CG
              257  CGCG                
AseI         1356  ATTAAT               AT^TAAT
             2484  ATTAAT              
AsnI         1356  ATTAAT               AT^TAAT
             2484  ATTAAT              
BanI          148  GGCGCC               G^G[CT][GA]CC
              783  GGCACC              
BciVI         829  GGATAC               GTATCC(6/5)
             1843  GGATAC              
BglI          295  GCCTGCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1130  GCCAGCTAGGC         
BsaAI        1489  CACGTG               [CT]AC^GT[GA]
             2344  TACGTG              
BsaI         1411  GAGACC               GGTCTC(1/5)
             2168  GAGACC              
BseRI         307  GAGGAG               GAGGAG(10/8)
             1312  GAGGAG              
BsmI         1583  GAATGC               GAATGC(1/-1)
             2105  GCATTC              
BsrDI         929  CATTGC               GCAATG(2/0)
             1396  GCAATG              
BstUI         102  CGCG                 CG^CG
              257  CGCG                
MfeI         1838  CAATTG               C^AATTG
             2391  CAATTG              
MunI         1838  CAATTG               C^AATTG
             2391  CAATTG              
MvnI          102  CGCG                 CG^CG
              257  CGCG                
PshBI        1356  ATTAAT               AT^TAAT
             2484  ATTAAT              
SapI         1462  GAAGAGC              GCTCTTC(1/4)
             2217  GAAGAGC             
TaqI         2043  TCGA                 T^CGA
             2085  TCGA                
TspRI         170  CAGTG                CA[GC]TG[ACGT][ACGT]^
              948  CACTG               
TthHB8I      2043  TCGA                 T^CGA
             2085  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AvaI        AvaII       BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BsiYI       BslI        
BsmAI       BsoBI       Bsp1286I    BspMI       BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        TfiI        Tru9I       TseI        
Tsp509I     XhoII       XmnI        
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