Restriction map for pF1KA1526
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AgeI        Alw44I      ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
BamHI       BciVI       BclI        BglII       BsaBI       
BseAI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraI        
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tsp509I     Tth111I     Van91I      XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AflII        1036  CTTAAG               C^TTAAG
AflIII        500  ACGTGT               A^C[GA][CT]GT
AocI          771  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       132  AGCGCT               AGC^GCT
ApaI         1218  GGGCCC               GGGCC^C
AviII         224  TGCGCA               TGC^GCA
AvrII        2521  CCTAGG               C^CTAGG
BalI         2127  TGGCCA               TGG^CCA
BbrPI        2584  CACGTG               CAC^GTG
BbvCI        1485  GCTGAGG              CCTCAGC(-5/-2)
BfrI         1036  CTTAAG               C^TTAAG
BlnI         2521  CCTAGG               C^CTAGG
BsiWI         114  CGTACG               C^GTACG
BsrBI         160  GAGCGG               CCGCTC(-3/-3)
BssHII        199  GCGCGC               G^CGCGC
BstAPI        807  GCACCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         632  CCAAGAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1708  AGATCC               [GA]^GATC[CT]
Bsu36I        771  CCTGAGG              CC^T[ACGT]AGG
Eco47III      132  AGCGCT               AGC^GCT
Eco81I        771  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1425  CCTCTCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI          224  TGCGCA               TGC^GCA
HindIII      1731  AAGCTT               A^AGCTT
MflI         1708  AGATCC               [GA]^GATC[CT]
MluNI        2127  TGGCCA               TGG^CCA
MscI         2127  TGGCCA               TGG^CCA
MseI         1037  TTAA                 T^TAA
NaeI         1225  GCCGGC               GCC^GGC
NgoMIV       1225  GCCGGC               G^CCGGC
PmaCI        2584  CACGTG               CAC^GTG
PmlI         2584  CACGTG               CAC^GTG
PstI         2078  CTGCAG               CTGCA^G
SacI           30  GAGCTC               GAGCT^C
SapI          312  GCTCTTC              GCTCTTC(1/4)
StuI         2657  AGGCCT               AGG^CCT
Tru9I        1037  TTAA                 T^TAA
XbaI          978  TCTAGA               T^CTAGA
XhoII        1708  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AatII        1072  GACGTC               GACGT^C
             2200  GACGTC              
BbeI          263  GGCGCC               GGCGC^C
             2497  GGCGCC              
BbsI          211  GTCTTC               GAAGAC(2/6)
             1858  GTCTTC              
BcgI          206  GCAACGTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2480  GCATCGCCATCG        
BlpI          147  GCTGAGC              GC^T[ACGT]AGC
             2549  GCTCAGC             
Bpu1102I      147  GCTGAGC              GC^T[ACGT]AGC
             2549  GCTCAGC             
BpuAI         211  GTCTTC               GAAGAC(2/6)
             1858  GTCTTC              
BsaAI         499  TACGTG               [CT]AC^GT[GA]
             2584  CACGTG              
BsaWI         252  TCCGGT               [AT]^CCGG[AT]
              791  ACCGGA              
BsmBI        1359  CGTCTC               CGTCTC(1/5)
             1959  CGTCTC              
BspMI         344  ACCTGC               ACCTGC(4/8)
             1295  ACCTGC              
BssSI         445  CACGAG               CACGAG(-5/-1)
             1265  CACGAG              
CelII         147  GCTGAGC              GC^T[ACGT]AGC
             2549  GCTCAGC             
DraIII        484  CACGGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1477  CACCTGGTG           
EarI          313  CTCTTC               CTCTTC(1/4)
             1938  CTCTTC              
HincII        571  GTCAAC               GT[CT]^[GA]AC
             2014  GTCAAC              
HindII        571  GTCAAC               GT[CT]^[GA]AC
             2014  GTCAAC              
KasI          263  GGCGCC               G^GCGCC
             2497  GGCGCC              
Ksp632I       313  CTCTTC               CTCTTC(1/4)
             1938  CTCTTC              
NarI          263  GGCGCC               GG^CGCC
             2497  GGCGCC              
PleI         2274  GACTC                GAGTC(4/5)
             1501  GAGTC               
PpuMI        1814  AGGACCT              [GA]G^G[AT]CC[CT]
             1912  GGGACCT             
PvuII         121  CAGCTG               CAG^CTG
              307  CAGCTG              
SfoI          263  GGCGCC               GGC^GCC
             2497  GGCGCC              
SmaI          590  CCCGGG               CCC^GGG
             1531  CCCGGG              
XmaCI         590  CCCGGG               C^CCGGG
             1531  CCCGGG              
XmaI          590  CCCGGG               C^CCGGG
             1531  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AhdI        
AluI        AlwI        AlwNI       AspEI       AspHI       
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrFI       BsrI        
BssKI       BstEII      BstF5I      BstNI       BstPI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco52I      
EcoO109I    EcoO65I     EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SexAI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        TseI        Tsp45I      TspRI       TthHB8I     
XcmI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com