Restriction map for pF1KA1526
 (Linear) (Six-base) MAPSORT of: KIAA1526.seq  Check: 2160  from: 1  to: 2721



KIAA1526.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1304    2721 
   Size:       1304    1417
 
AatII G_ACGT'C

Cuts at:      0    1076    2204    2721 
   Size:       1076    1128     517

  Fragments arranged by size: 

                1128    1076     517
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     444     643     959    2721 
   Size:        444     199     316    1762

  Fragments arranged by size: 

                1762     444     316     199
 
AfeI AGC'GCT

Cuts at:      0     134    2721 
   Size:        134    2587
 
AflII C'TTAA_G

Cuts at:      0    1036    2721 
   Size:       1036    1685
 
AflIII A'CryG_T

Cuts at:      0     500    2721 
   Size:        500    2221
 
AhdI GACnn_n'nnGTC

Cuts at:      0      21     547     861    2721 
   Size:         21     526     314    1860

  Fragments arranged by size: 

                1860     526     314      21
 
AleI CACnn'nnGTG

Cuts at:      0     706     764     922    1102    2561    2721 
   Size:        706      58     158     180    1459     160

  Fragments arranged by size: 

                1459     706     180     160     158      58
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2392    2721 
   Size:       2392     329
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1113    1145    2721 
   Size:       1113      32    1576

  Fragments arranged by size: 

                1576    1113      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     564     937    1725    2095    2517    2721 
   Size:        564     373     788     370     422     204

  Fragments arranged by size: 

                 788     564     422     373     370     204
 
ApaI G_GGCC'C

Cuts at:      0    1222    2721 
   Size:       1222    1499
 
AvaI C'yCGr_G

Cuts at:      0     590     741    1531    1848    2721 
   Size:        590     151     790     317     873

  Fragments arranged by size: 

                 873     790     590     317     151
 
AvrII C'CTAG_G

Cuts at:      0    2521    2721 
   Size:       2521     200
 
BanI G'GyrC_C

Cuts at:      0     263     766    1049    1292    1441    1597    1894    2497 
   Size:        263     503     283     243     149     156     297     603

Cuts at:   2497    2721 
   Size:        224

  Fragments arranged by size: 

                 603     503     297     283     263     243     224     156
                 149
 
BanII G_rGCy'C

Cuts at:      0      34      58     478     631     959    1222    2435    2721 
   Size:         34      24     420     153     328     263    1213     286

  Fragments arranged by size: 

                1213     420     328     286     263     153      34      24
            
 
BbeI G_GCGC'C

Cuts at:      0     267    2501    2721 
   Size:        267    2234     220

  Fragments arranged by size: 

                2234     267     220
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     204    1851    2721 
   Size:        204    1647     870

  Fragments arranged by size: 

                1647     870     204
 
BbvCI CC'TCA_GC

Cuts at:      0    1486    2721 
   Size:       1486    1235
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     195     229    2469    2503    2721 
   Size:        195      34    2240      34     218

  Fragments arranged by size: 

                2240     218     195      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     616    1226    1324    2005    2092    2721 
   Size:        616     610      98     681      87     629

  Fragments arranged by size: 

                 681     629     616     610      98      87
 
BlpI GC'TnA_GC

Cuts at:      0     148    2550    2721 
   Size:        148    2402     171

  Fragments arranged by size: 

                2402     171     148
 
Bme1580I G_kGCm'C

Cuts at:      0     771    1222    1855    1897    2721 
   Size:        771     451     633      42     824

  Fragments arranged by size: 

                 824     771     633     451      42
 
BmgBI CAC'GTC

Cuts at:      0    2438    2721 
   Size:       2438     283
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1834    2721 
   Size:       1834     887
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1297    1660    1804    2449    2721 
   Size:       1297     363     144     645     272

  Fragments arranged by size: 

                1297     645     363     272     144
 
Bpu10I CC'TnA_GC

Cuts at:      0    1243    1486    2721 
   Size:       1243     243    1235

  Fragments arranged by size: 

                1243    1235     243
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     943    2557    2721 
   Size:        943    1614     164

  Fragments arranged by size: 

                1614     943     164
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1105    1138    1563    2721 
   Size:       1105      33     425    1158

  Fragments arranged by size: 

                1158    1105     425      33
 
BsaAI yAC'GTr

Cuts at:      0     501    2586    2721 
   Size:        501    2085     135

  Fragments arranged by size: 

                2085     501     135
 
BsaHI Gr'CG_yC

Cuts at:      0     264    1073    1997    2201    2498    2721 
   Size:        264     809     924     204     297     223

  Fragments arranged by size: 

                 924     809     297     264     223     204
 
BsaWI w'CCGG_w

Cuts at:      0     252     791    2721 
   Size:        252     539    1930

  Fragments arranged by size: 

                1930     539     252
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1534    1564    1585    1615    1963    1993    2422    2452 
   Size:       1534      30      21      30     348      30     429      30

Cuts at:   2452    2721 
   Size:        269

  Fragments arranged by size: 

                1534     429     348     269      30      30      30      30
                  21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     810    1294    1414    1908    2721 
   Size:        810     484     120     494     813

  Fragments arranged by size: 

                 813     810     494     484     120
 
BseYI C'CCAG_C

Cuts at:      0      51     752     849    1221    1537    1803    1975    2095 
   Size:         51     701      97     372     316     266     172     120

Cuts at:   2095    2252    2721 
   Size:        157     469

  Fragments arranged by size: 

                 701     469     372     316     266     172     157     120
                  97      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     790    1907    2721 
   Size:        109     681    1117     814

  Fragments arranged by size: 

                1117     814     681     109
 
BsiEI CG_ry'CG

Cuts at:      0      65     332     861    2721 
   Size:         65     267     529    1860

  Fragments arranged by size: 

                1860     529     267      65
 
BsiHKAI G_wGCw'C

Cuts at:      0      34     485     802    2630    2721 
   Size:         34     451     317    1828      91

  Fragments arranged by size: 

                1828     451     317      91      34
 
BsiWI C'GTAC_G

Cuts at:      0     114    2721 
   Size:        114    2607
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1365    1965    2721 
   Size:       1365     600     756

  Fragments arranged by size: 

                1365     756     600
 
Bsp1286I G_dGCh'C

Cuts at:      0      34      58     478     485     631     771     802     959 
   Size:         34      24     420       7     146     140      31     157

Cuts at:    959    1222    1855    1897    2435    2630    2721 
   Size:        263     633      42     538     195      91

  Fragments arranged by size: 

                 633     538     420     263     195     157     146     140
                  91      42      34      31      24       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     353    1304    2721 
   Size:        353     951    1417

  Fragments arranged by size: 

                1417     951     353
 
BsrBI CCG'CTC

Cuts at:      0     162    2721 
   Size:        162    2559
 
BsrFI r'CCGG_y

Cuts at:      0      43     860    1225    2721 
   Size:         43     817     365    1496

  Fragments arranged by size: 

                1496     817     365      43
 
BssHII G'CGCG_C

Cuts at:      0     199    2721 
   Size:        199    2522
 
BssSI C'ACGA_G

Cuts at:      0     445    1265    2721 
   Size:        445     820    1456

  Fragments arranged by size: 

                1456     820     445
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     813    2721 
   Size:        813    1908
 
BstEII G'GTnAC_C

Cuts at:      0     594     787    1236    2721 
   Size:        594     193     449    1485

  Fragments arranged by size: 

                1485     594     449     193
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     639    2721 
   Size:        639    2082
 
BstYI r'GATC_y

Cuts at:      0    1708    2721 
   Size:       1708    1013
 
Bsu36I CC'TnA_GG

Cuts at:      0     772    2721 
   Size:        772    1949
 
BtgI C'CryG_G

Cuts at:      0     468     759     882    1148    1322    1347    1553    1593 
   Size:        468     291     123     266     174      25     206      40

Cuts at:   1593    1694    2041    2721 
   Size:        101     347     680

  Fragments arranged by size: 

                 680     468     347     291     266     206     174     123
                 101      40      25
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2466    2721 
   Size:       2466     255
 
BtsI GCAGTG_nn'

Cuts at:      0     177    1741    2721 
   Size:        177    1564     980

  Fragments arranged by size: 

                1564     980     177
 
DraIII CAC_nnn'GTG

Cuts at:      0     489    1482    2721 
   Size:        489     993    1239

  Fragments arranged by size: 

                1239     993     489
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     253    1024    2695    2721 
   Size:        253     771    1671      26

  Fragments arranged by size: 

                1671     771     253      26
 
EaeI y'GGCC_r

Cuts at:      0      62     329     858    1619    2127    2468    2721 
   Size:         62     267     529     761     508     341     253

  Fragments arranged by size: 

                 761     529     508     341     267     253      62
 
EagI C'GGCC_G

Cuts at:      0      62     329     858    2721 
   Size:         62     267     529    1863

  Fragments arranged by size: 

                1863     529     267      62
 
EarI CTCTTCn'nnn_

Cuts at:      0     319    1944    2721 
   Size:        319    1625     777

  Fragments arranged by size: 

                1625     777     319
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     172    1008    2721 
   Size:        172     836    1713

  Fragments arranged by size: 

                1713     836     172
 
EcoICRI GAG'CTC

Cuts at:      0      32    2721 
   Size:         32    2689
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     444     643     959    1297    1660    1804    2449    2721 
   Size:        444     199     316     338     363     144     645     272

  Fragments arranged by size: 

                 645     444     363     338     316     272     199     144
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1429    2721 
   Size:       1429    1292
 
EcoO109I rG'GnC_Cy

Cuts at:      0     336    1218    1371    1528    1815    1913    2721 
   Size:        336     882     153     157     287      98     808

  Fragments arranged by size: 

                 882     808     336     287     157     153      98
 
FspI TGC'GCA

Cuts at:      0     226    2721 
   Size:        226    2495
 
FspAI rTGC'GCAy

Cuts at:      0     226    2721 
   Size:        226    2495
 
HaeII r_GCGC'y

Cuts at:      0     136     267    2221    2324    2501    2721 
   Size:        136     131    1954     103     177     220

  Fragments arranged by size: 

                1954     220     177     136     131     103
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       7      39     533     565     847     879     919     951 
   Size:          7      32     494      32     282      32      40      32

Cuts at:    951    1486    1518    1534    1566    1693    1725    1921    1953 
   Size:        535      32      16      32     127      32     196      32

Cuts at:   1953    2422    2454    2721 
   Size:        469      32     267

  Fragments arranged by size: 

                 535     494     469     282     267     196     127      40
                  32      32      32      32      32      32      32      32
                  32      16       7
 
HincII GTy'rAC

Cuts at:      0     573    2016    2721 
   Size:        573    1443     705

  Fragments arranged by size: 

                1443     705     573
 
HindIII A'AGCT_T

Cuts at:      0    1731    2721 
   Size:       1731     990
 
KasI G'GCGC_C

Cuts at:      0     263    2497    2721 
   Size:        263    2234     224

  Fragments arranged by size: 

                2234     263     224
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     326    2721 
   Size:        326    2395
 
MscI TGG'CCA

Cuts at:      0    2129    2721 
   Size:       2129     592
 
MslI CAynn'nnrTG

Cuts at:      0     499     706     764     922    1102    2561    2721 
   Size:        499     207      58     158     180    1459     160

  Fragments arranged by size: 

                1459     499     207     180     160     158      58
 
MspA1I CmG'CkG

Cuts at:      0      12     123     309     953    1891    1992    2267    2721 
   Size:         12     111     186     644     938     101     275     454

  Fragments arranged by size: 

                 938     644     454     275     186     111     101      12
            
 
NaeI GCC'GGC

Cuts at:      0    1227    2721 
   Size:       1227    1494
 
NarI GG'CG_CC

Cuts at:      0     264    2498    2721 
   Size:        264    2234     223

  Fragments arranged by size: 

                2234     264     223
 
NcoI C'CATG_G

Cuts at:      0    1148    1322    1553    2721 
   Size:       1148     174     231    1168

  Fragments arranged by size: 

                1168    1148     231     174
 
NgoMIV G'CCGG_C

Cuts at:      0    1225    2721 
   Size:       1225    1496
 
PasI CC'CwG_GG

Cuts at:      0     673     867    1719    1909    2475    2721 
   Size:        673     194     852     190     566     246

  Fragments arranged by size: 

                 852     673     566     246     194     190
 
PfoI T'CCnGG_A

Cuts at:      0    1132    2721 
   Size:       1132    1589
 
PmlI CAC'GTG

Cuts at:      0    2586    2721 
   Size:       2586     135
 
PpuMI rG'GwC_Cy

Cuts at:      0    1815    1913    2721 
   Size:       1815      98     808

  Fragments arranged by size: 

                1815     808      98
 
PspOMI G'GGCC_C

Cuts at:      0    1218    2721 
   Size:       1218    1503
 
PstI C_TGCA'G

Cuts at:      0    2082    2721 
   Size:       2082     639
 
PvuII CAG'CTG

Cuts at:      0     123     309    2721 
   Size:        123     186    2412

  Fragments arranged by size: 

                2412     186     123
 
SacI G_AGCT'C

Cuts at:      0      34    2721 
   Size:         34    2687
 
SapI GCTCTTCn'nnn_

Cuts at:      0     319    2721 
   Size:        319    2402
 
SexAI A'CCwGG_T

Cuts at:      0     218     842    1478    2721 
   Size:        218     624     636    1243

  Fragments arranged by size: 

                1243     636     624     218
 
SfcI C'TryA_G

Cuts at:      0     363    1563    2078    2721 
   Size:        363    1200     515     643

  Fragments arranged by size: 

                1200     643     515     363
 
SfoI GGC'GCC

Cuts at:      0     265    2499    2721 
   Size:        265    2234     222

  Fragments arranged by size: 

                2234     265     222
 
SmaI CCC'GGG

Cuts at:      0     592    1533    2721 
   Size:        592     941    1188

  Fragments arranged by size: 

                1188     941     592
 
SmlI C'TyrA_G

Cuts at:      0     958    1036    2572    2721 
   Size:        958      78    1536     149

  Fragments arranged by size: 

                1536     958     149      78
 
StuI AGG'CCT

Cuts at:      0    2659    2721 
   Size:       2659      62
 
StyI C'CwwG_G

Cuts at:      0     437     903    1148    1322    1553    1767    1795    2521 
   Size:        437     466     245     174     231     214      28     726

Cuts at:   2521    2721 
   Size:        200

  Fragments arranged by size: 

                 726     466     437     245     231     214     200     174
                  28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     190     696    1420    1917    2147    2721 
   Size:        190     506     724     497     230     574

  Fragments arranged by size: 

                 724     574     506     497     230     190
 
TatI w'GTAC_w

Cuts at:      0     653    2721 
   Size:        653    2068
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1585    1617    2721 
   Size:       1585      32    1104

  Fragments arranged by size: 

                1585    1104      32
 
XbaI T'CTAG_A

Cuts at:      0     978    2721 
   Size:        978    1743
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      48     282     760    1629    1989    2442    2721 
   Size:         48     234     478     869     360     453     279

  Fragments arranged by size: 

                 869     478     453     360     279     234      48
 
XmaI C'CCGG_G

Cuts at:      0     590    1531    2721 
   Size:        590     941    1190

  Fragments arranged by size: 

                1190     941     590
 
ZraI GAC'GTC

Cuts at:      0    1074    2202    2721 
   Size:       1074    1128     519

  Fragments arranged by size: 

                1128    1074     519
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI    AflII   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI     AvaI    AvrII     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BglI     BlpI Bme1580I    BmgBI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     PasI
     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI     SapI
    SexAI     SfcI     SfoI     SmaI     SmlI     StuI     StyI    TaqII
     TatI     TstI     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AgeI    ApaLI     ApoI     AscI     AseI
    AsiSI     BaeI    BamHI    BciVI     BclI    BfrBI    BglII     BmrI
     BmtI    BsaBI     BsmI    BspEI    BspHI    BsrDI    BsrGI    BstBI
  BstZ17I     ClaI    CspCI     DraI    EcoRI    EcoRV     FalI     FseI
     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI  Tth111I     XhoI     XmnI
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