Restriction map for
pF1KA0382
(Linear) (Six-base) MAPSORT of: KIAA0382.seq Check: 1005 from: 1 to: 4632
KIAA0382.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 699 3728 4632
Size: 699 3029 904
Fragments arranged by size:
3029 904 699
AccI GT'mk_AC
Cuts at: 0 16 4632
Size: 16 4616
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1994 2437 2797 3016 4632
Size: 497 1497 443 360 219 1616
Fragments arranged by size:
1616 1497 497 443 360 219
AfeI AGC'GCT
Cuts at: 0 4435 4632
Size: 4435 197
AflIII A'CryG_T
Cuts at: 0 1549 3116 3221 4632
Size: 1549 1567 105 1411
Fragments arranged by size:
1567 1549 1411 105
AlwNI CAG_nnn'CTG
Cuts at: 0 775 853 1972 2086 3352 3514 3786 4632
Size: 775 78 1119 114 1266 162 272 846
Fragments arranged by size:
1266 1119 846 775 272 162 114 78
ApoI r'AATT_y
Cuts at: 0 620 950 1689 2462 2489 3033 3762 4417
Size: 620 330 739 773 27 544 729 655
Cuts at: 4417 4632
Size: 215
Fragments arranged by size:
773 739 729 655 620 544 330 215
27
AscI GG'CGCG_CC
Cuts at: 0 1870 4632
Size: 1870 2762
AseI AT'TA_AT
Cuts at: 0 2375 4632
Size: 2375 2257
BanI G'GyrC_C
Cuts at: 0 4029 4632
Size: 4029 603
BanII G_rGCy'C
Cuts at: 0 1364 1624 2403 3029 4632
Size: 1364 260 779 626 1603
Fragments arranged by size:
1603 1364 779 626 260
BbsI GAAGACnn'nnnn_
Cuts at: 0 163 267 1359 1991 2761 3137 3833 4632
Size: 163 104 1092 632 770 376 696 799
Fragments arranged by size:
1092 799 770 696 632 376 163 104
BbvCI CC'TCA_GC
Cuts at: 0 740 4632
Size: 740 3892
BciVI GTATCCnnnnn_n'
Cuts at: 0 1969 2460 4632
Size: 1969 491 2172
Fragments arranged by size:
2172 1969 491
BclI T'GATC_A
Cuts at: 0 2427 2690 4632
Size: 2427 263 1942
Fragments arranged by size:
2427 1942 263
BglI GCCn_nnn'nGGC
Cuts at: 0 313 4632
Size: 313 4319
BglII A'GATC_T
Cuts at: 0 1338 2906 4632
Size: 1338 1568 1726
Fragments arranged by size:
1726 1568 1338
BmrI ACTGGGnnnn_n'
Cuts at: 0 874 899 2344 4261 4632
Size: 874 25 1445 1917 371
Fragments arranged by size:
1917 1445 874 371 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 403 557 789 875 2108 2973 4632
Size: 338 65 154 232 86 1233 865 1659
Fragments arranged by size:
1659 1233 865 338 232 154 86 65
Bpu10I CC'TnA_GC
Cuts at: 0 740 779 1358 1598 1880 3028 4632
Size: 740 39 579 240 282 1148 1604
Fragments arranged by size:
1604 1148 740 579 282 240 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1288 4632
Size: 1288 3344
BsaI GGTCTCn'nnnn_
Cuts at: 0 1809 2563 4632
Size: 1809 754 2069
Fragments arranged by size:
2069 1809 754
BsaAI yAC'GTr
Cuts at: 0 3616 4632
Size: 3616 1016
BsaBI GATnn'nnATC
Cuts at: 0 1379 3549 4172 4632
Size: 1379 2170 623 460
Fragments arranged by size:
2170 1379 623 460
BsaHI Gr'CG_yC
Cuts at: 0 438 4632
Size: 438 4194
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 779 809 1658 1688 4632
Size: 779 30 849 30 2944
Fragments arranged by size:
2944 849 779 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 138 190 515 2182 2644 3505 4306 4361
Size: 138 52 325 1667 462 861 801 55
Cuts at: 4361 4470 4632
Size: 109 162
Fragments arranged by size:
1667 861 801 462 325 162 138 109
55 52
BseYI C'CCAG_C
Cuts at: 0 1792 4436 4632
Size: 1792 2644 196
Fragments arranged by size:
2644 1792 196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1125 1574 2182 4291 4508 4632
Size: 1125 449 608 2109 217 124
Fragments arranged by size:
2109 1125 608 449 217 124
BsiHKAI G_wGCw'C
Cuts at: 0 578 1364 1624 1700 2403 3029 4139 4632
Size: 578 786 260 76 703 626 1110 493
Fragments arranged by size:
1110 786 703 626 578 493 260 76
Bsp1286I G_dGCh'C
Cuts at: 0 578 1364 1624 1700 2403 3029 4139 4632
Size: 578 786 260 76 703 626 1110 493
Fragments arranged by size:
1110 786 703 626 578 493 260 76
BspHI T'CATG_A
Cuts at: 0 515 1827 3063 4632
Size: 515 1312 1236 1569
Fragments arranged by size:
1569 1312 1236 515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 699 1988 3728 4632
Size: 699 1289 1740 904
Fragments arranged by size:
1740 1289 904 699
BsrBI CCG'CTC
Cuts at: 0 1545 4632
Size: 1545 3087
BsrDI GCAATG_nn'
Cuts at: 0 113 3962 4632
Size: 113 3849 670
Fragments arranged by size:
3849 670 113
BssHII G'CGCG_C
Cuts at: 0 1870 4632
Size: 1870 2762
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3751 4632
Size: 3751 881
BstBI TT'CG_AA
Cuts at: 0 2220 2409 2556 4632
Size: 2220 189 147 2076
Fragments arranged by size:
2220 2076 189 147
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1800 2303 3080 4632
Size: 1800 503 777 1552
Fragments arranged by size:
1800 1552 777 503
BstYI r'GATC_y
Cuts at: 0 184 713 849 1338 1374 2906 3471 4466
Size: 184 529 136 489 36 1532 565 995
Cuts at: 4466 4632
Size: 166
Fragments arranged by size:
1532 995 565 529 489 184 166 136
36
Bsu36I CC'TnA_GG
Cuts at: 0 1370 1919 4632
Size: 1370 549 2713
Fragments arranged by size:
2713 1370 549
BtgI C'CryG_G
Cuts at: 0 1810 1853 3982 4009 4100 4632
Size: 1810 43 2129 27 91 532
Fragments arranged by size:
2129 1810 532 91 43 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1366 4632
Size: 1366 3266
BtsI GCAGTG_nn'
Cuts at: 0 891 4632
Size: 891 3741
ClaI AT'CG_AT
Cuts at: 0 1293 2582 4632
Size: 1293 1289 2050
Fragments arranged by size:
2050 1293 1289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2810 2845 4632
Size: 2810 35 1787
Fragments arranged by size:
2810 1787 35
DraIII CAC_nnn'GTG
Cuts at: 0 4165 4632
Size: 4165 467
DrdI GACnn_nn'nnGTC
Cuts at: 0 271 1823 4632
Size: 271 1552 2809
Fragments arranged by size:
2809 1552 271
EaeI y'GGCC_r
Cuts at: 0 2857 4632
Size: 2857 1775
EarI CTCTTCn'nnn_
Cuts at: 0 1776 3017 3808 4202 4632
Size: 1776 1241 791 394 430
Fragments arranged by size:
1776 1241 791 430 394
EcoICRI GAG'CTC
Cuts at: 0 1362 1622 2401 3027 4632
Size: 1362 260 779 626 1605
Fragments arranged by size:
1605 1362 779 626 260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 403 497 557 789 875 1994 2108
Size: 338 65 94 60 232 86 1119 114
Cuts at: 2108 2437 2797 2973 3016 4632
Size: 329 360 176 43 1616
Fragments arranged by size:
1616 1119 360 338 329 232 176 114
94 86 65 60 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3449 4632
Size: 3449 1183
EcoO109I rG'GnC_Cy
Cuts at: 0 1804 2015 3372 4632
Size: 1804 211 1357 1260
Fragments arranged by size:
1804 1357 1260 211
EcoRI G'AATT_C
Cuts at: 0 2462 4632
Size: 2462 2170
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2530 3154 4135 4632
Size: 2530 624 981 497
Fragments arranged by size:
2530 981 624 497
HaeII r_GCGC'y
Cuts at: 0 2958 3320 4437 4632
Size: 2958 362 1117 195
Fragments arranged by size:
2958 1117 362 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 169 183 201 215 318 350
Size: 133 32 4 14 18 14 103 32
Cuts at: 350 510 542 742 774 939 971 991 1023
Size: 160 32 200 32 165 32 20 32
Cuts at: 1023 1315 1346 1347 1378 1581 1606 1613 1638
Size: 292 31 1 31 203 25 7 25
Cuts at: 1638 2041 2073 2682 2714 4162 4194 4458 4490
Size: 403 32 609 32 1448 32 264 32
Cuts at: 4490 4589 4603 4621 4632
Size: 99 14 18 11
Fragments arranged by size:
1448 609 403 292 264 203 200 165
160 133 103 99 32 32 32 32
32 32 32 32 32 32 31 31
25 25 20 18 18 14 14 14
11 7 4 1
HincII GTy'rAC
Cuts at: 0 3440 4200 4632
Size: 3440 760 432
Fragments arranged by size:
3440 760 432
HindIII A'AGCT_T
Cuts at: 0 3284 4632
Size: 3284 1348
HpaI GTT'AAC
Cuts at: 0 4200 4632
Size: 4200 432
MfeI C'AATT_G
Cuts at: 0 1841 4632
Size: 1841 2791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 996 1690 2049 2810 3364 3663 4632
Size: 996 694 359 761 554 299 969
Fragments arranged by size:
996 969 761 694 554 359 299
MscI TGG'CCA
Cuts at: 0 2859 4632
Size: 2859 1773
MslI CAynn'nnrTG
Cuts at: 0 346 1651 2797 3301 3662 3923 4078 4166
Size: 346 1305 1146 504 361 261 155 88
Cuts at: 4166 4388 4632
Size: 222 244
Fragments arranged by size:
1305 1146 504 361 346 261 244 222
155 88
MspA1I CmG'CkG
Cuts at: 0 744 1109 2618 3208 4632
Size: 744 365 1509 590 1424
Fragments arranged by size:
1509 1424 744 590 365
NcoI C'CATG_G
Cuts at: 0 1853 4100 4632
Size: 1853 2247 532
Fragments arranged by size:
2247 1853 532
NdeI CA'TA_TG
Cuts at: 0 503 3935 4632
Size: 503 3432 697
Fragments arranged by size:
3432 697 503
NspI r_CATG'y
Cuts at: 0 1553 2647 4632
Size: 1553 1094 1985
Fragments arranged by size:
1985 1553 1094
PciI A'CATG_T
Cuts at: 0 1549 4632
Size: 1549 3083
PflMI CCAn_nnn'nTGG
Cuts at: 0 4509 4590 4632
Size: 4509 81 42
Fragments arranged by size:
4509 81 42
PfoI T'CCnGG_A
Cuts at: 0 852 4632
Size: 852 3780
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2390 2422 3846 3878 4632
Size: 2390 32 1424 32 754
Fragments arranged by size:
2390 1424 754 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1804 3372 4632
Size: 1804 1568 1260
Fragments arranged by size:
1804 1568 1260
PshAI GACnn'nnGTC
Cuts at: 0 504 4632
Size: 504 4128
PstI C_TGCA'G
Cuts at: 0 1338 2892 4319 4632
Size: 1338 1554 1427 313
Fragments arranged by size:
1554 1427 1338 313
PvuII CAG'CTG
Cuts at: 0 744 1109 3208 4632
Size: 744 365 2099 1424
Fragments arranged by size:
2099 1424 744 365
SacI G_AGCT'C
Cuts at: 0 1364 1624 2403 3029 4632
Size: 1364 260 779 626 1603
Fragments arranged by size:
1603 1364 779 626 260
SanDI GG'GwC_CC
Cuts at: 0 1804 4632
Size: 1804 2828
SapI GCTCTTCn'nnn_
Cuts at: 0 3017 3808 4632
Size: 3017 791 824
Fragments arranged by size:
3017 824 791
SfcI C'TryA_G
Cuts at: 0 658 791 879 1334 1604 1861 2003 2888
Size: 658 133 88 455 270 257 142 885
Cuts at: 2888 3203 4315 4632
Size: 315 1112 317
Fragments arranged by size:
1112 885 658 455 317 315 270 257
142 133 88
SmlI C'TyrA_G
Cuts at: 0 1267 4632
Size: 1267 3365
SpeI A'CTAG_T
Cuts at: 0 565 4632
Size: 565 4067
SspI AAT'ATT
Cuts at: 0 2838 3906 4632
Size: 2838 1068 726
Fragments arranged by size:
2838 1068 726
StuI AGG'CCT
Cuts at: 0 3913 4632
Size: 3913 719
StyI C'CwwG_G
Cuts at: 0 1475 1853 4100 4632
Size: 1475 378 2247 532
Fragments arranged by size:
2247 1475 532 378
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1538 2313 4366 4632
Size: 1538 775 2053 266
Fragments arranged by size:
2053 1538 775 266
TatI w'GTAC_w
Cuts at: 0 321 861 3247 4514 4632
Size: 321 540 2386 1267 118
Fragments arranged by size:
2386 1267 540 321 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 347 379 1658 1690 4632
Size: 347 32 1279 32 2942
Fragments arranged by size:
2942 1279 347 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 162 3883 4632
Size: 162 3721 749
Fragments arranged by size:
3721 749 162
XbaI T'CTAG_A
Cuts at: 0 957 1287 1341 4632
Size: 957 330 54 3291
Fragments arranged by size:
3291 957 330 54
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4591 4632
Size: 4591 41
XmnI GAAnn'nnTTC
Cuts at: 0 986 1445 2462 2510 3134 4632
Size: 986 459 1017 48 624 1498
Fragments arranged by size:
1498 1017 986 624 459 48
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlwNI ApoI AscI
AseI BanI BanII BbsI BbvCI BciVI BclI BglI
BglII BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BssHII BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI
MslI MspA1I NcoI NdeI NspI PciI PflMI PfoI
PpiI PpuMI PshAI PstI PvuII SacI SanDI SapI
SfcI SmlI SpeI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI AsiSI AvaI AvrII BaeI BamHI
BbeI BcgI BfrBI BlpI Bme1580I BmgBI BmtI BplI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrFI BsrGI
BssSI BstEII BstZ17I DraI EagI EciI EcoRV FseI
FspI FspAI KasI KpnI MluI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SwaI XhoI XmaI ZraI