Restriction map for pF1KB5204
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspHI       
AspI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
Bst1107I    BstBI       BstF5I      BstNI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRII      
EcoRV       EcoT22I     FbaI        FokI        FseI        
FspI        HgaI        Hin1I       HindIII     KasI        
KpnI        KspI        MaeI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MunI        MvaI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AhdI          346  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          609  GATCC                GGATC(4/5)
ApaI          475  GGGCCC               GGGCC^C
AspEI         346  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI          416  GGCACC               G^G[CT][GA]CC
BanII         475  GGGCCC               G[GA]GC[CT]^C
BbsI          189  GAAGAC               GAAGAC(2/6)
BpuAI         189  GAAGAC               GAAGAC(2/6)
BsrFI         419  ACCGGC               [GA]^CCGG[CT]
BstAPI         75  GCAGCCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        726  GGTCACC              G^GT[ACGT]ACC
BstPI         726  GGTCACC              G^GT[ACGT]ACC
BstXI         174  CCACATCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        419  ACCGGC               [GA]^CCGG[CT]
DdeI          796  CTCAG                C^T[ACGT]AG
EaeI          712  CGGCCA               [CT]^GGCC[GA]
Eam1105I      346  GACCCCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          630  CTCTTC               CTCTTC(1/4)
EcoO65I       726  GGTCACC              G^GT[ACGT]ACC
EcoRI         124  GAATTC               G^AATTC
HaeII         109  GGCGCT               [GA]GCGC^[CT]
HincII        615  GTTAAC               GT[CT]^[GA]AC
HindII        615  GTTAAC               GT[CT]^[GA]AC
HpaI          615  GTTAAC               GTT^AAC
Ksp632I       630  CTCTTC               CTCTTC(1/4)
MseI          616  TTAA                 T^TAA
NcoI          515  CCATGG               C^CATGG
NspI          158  GCATGT               [GA]CATG^[CT]
PpuMI         705  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          411  CTGCAG               CTGCA^G
PvuII          64  CAGCTG               CAG^CTG
SfcI          411  CTGCAG               C^T[GA][CT]AG
TfiI          461  GATTC                G^A[AT]TC
Tru9I         616  TTAA                 T^TAA
Tsp45I        727  GTCAC                ^GT[GC]AC
XcmI          776  CCAAAGGGCACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII          51  CGCG                 CG^CG
              693  CGCG                
AcsI          124  GAATTC               [GA]^AATT[CT]
              487  AAATTC              
AluI           65  AGCT                 AG^CT
              138  AGCT                
AlwNI          61  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
               76  CAGCCGCTG           
ApoI          124  GAATTC               [GA]^AATT[CT]
              487  AAATTC              
AvaI          280  CCCGAG               C^[CT]CG[GA]G
              349  CCCGAG              
BmyI          475  GGGCCC               G[AGT]GC[ACT]^C
              781  GGGCAC              
BpmI          560  CTCCAG               CTGGAG(16/14)
              718  CTGGAG              
BsgI          337  CTGCAC               GTGCAG(16/14)
              312  GTGCAG              
BsoBI         280  CCCGAG               C^[CT]CG[GA]G
              349  CCCGAG              
Bsp1286I      475  GGGCCC               G[AGT]GC[ACT]^C
              781  GGGCAC              
BspMI         657  GCAGGT               ACCTGC(4/8)
              723  GCAGGT              
BssSI         454  CTCGTG               CACGAG(-5/-1)
              480  CTCGTG              
BstUI          51  CGCG                 CG^CG
              693  CGCG                
DsaI          384  CCGTGG               C^C[GA][CT]GG
              515  CCATGG              
EcoT14I       373  CCTTGG               C^C[AT][AT]GG
              515  CCATGG              
FauI          654  CCCGC                CCCGC(4/6)
              694  GCGGG               
HphI          184  GGTGA                GGTGA(8/7)
              728  TCACC               
MboII         189  GAAGA                GAAGA(8/7)
              631  TCTTC               
MspA1I         64  CAGCTG               C[AC]G^C[GT]G
               79  CCGCTG              
MvnI           51  CGCG                 CG^CG
              693  CGCG                
SfaNI         276  GATGC                GCATC(5/9)
              770  GATGC               
StyI          373  CCTTGG               C^C[AT][AT]GG
              515  CCATGG              
TaqI          344  TCGA                 T^CGA
              501  TCGA                
TthHB8I       344  TCGA                 T^CGA
              501  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        AvaII       BbvI        BcnI        BsaJI       
BsiYI       BslI        BsmFI       BsrI        BssKI       
Cac8I       CfoI        Cfr13I      DpnII       DraII       
EcoO109I    Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        MaeIII      
MboI        MnlI        MslI        MspI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       TseI        Tsp509I     
TspRI       
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