Restriction map for pF1KB5139
 (Linear) (Six-base) MAPSORT of: KIBB5139.seq  Check: 868  from: 1  to: 1701



KIBB5139.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1219    1701 
   Size:       1219     482
 
AccI GT'mk_AC

Cuts at:      0     695    1701 
   Size:        695    1006
 
Acc65I G'GTAC_C

Cuts at:      0    1038    1701 
   Size:       1038     663
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1470    1554    1701 
   Size:       1470      84     147

  Fragments arranged by size: 

                1470     147      84
 
AfeI AGC'GCT

Cuts at:      0     730     814    1162    1701 
   Size:        730      84     348     539

  Fragments arranged by size: 

                 730     539     348      84
 
AleI CACnn'nnGTG

Cuts at:      0     130     359    1701 
   Size:        130     229    1342

  Fragments arranged by size: 

                1342     229     130
 
AlwNI CAG_nnn'CTG

Cuts at:      0      90    1701 
   Size:         90    1611
 
ApaI G_GGCC'C

Cuts at:      0      25      68     168     411     641    1077    1176    1701 
   Size:         25      43     100     243     230     436      99     525

  Fragments arranged by size: 

                 525     436     243     230     100      99      43      25
            
 
ApaLI G'TGCA_C

Cuts at:      0     592     769    1270    1701 
   Size:        592     177     501     431

  Fragments arranged by size: 

                 592     501     431     177
 
AvaI C'yCGr_G

Cuts at:      0     799     978    1701 
   Size:        799     179     723

  Fragments arranged by size: 

                 799     723     179
 
BanI G'GyrC_C

Cuts at:      0     145     233     497    1038    1621    1701 
   Size:        145      88     264     541     583      80

  Fragments arranged by size: 

                 583     541     264     145      88      80
 
BanII G_rGCy'C

Cuts at:      0      25      68     168     411     486     641     988    1028 
   Size:         25      43     100     243      75     155     347      40

Cuts at:   1028    1077    1176    1701 
   Size:         49      99     525

  Fragments arranged by size: 

                 525     347     243     155     100      99      75      49
                  43      40      25
 
BbeI G_GCGC'C

Cuts at:      0    1625    1701 
   Size:       1625      76
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1249    1701 
   Size:       1249     452
 
BbvCI CC'TCA_GC

Cuts at:      0     112     602    1701 
   Size:        112     490    1099

  Fragments arranged by size: 

                1099     490     112
 
BglI GCCn_nnn'nGGC

Cuts at:      0     517    1701 
   Size:        517    1184
 
Bme1580I G_kGCm'C

Cuts at:      0      25      68     150     168     180     411     596     641 
   Size:         25      43      82      18      12     231     185      45

Cuts at:    641     773     799    1077    1176    1274    1701 
   Size:        132      26     278      99      98     427

  Fragments arranged by size: 

                 427     278     231     185     132      99      98      82
                  45      43      26      25      18      12
 
BmgBI CAC'GTC

Cuts at:      0     128    1701 
   Size:        128    1573
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     132     555    1701 
   Size:        132     423    1146

  Fragments arranged by size: 

                1146     423     132
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     631    1177    1701 
   Size:        631     546     524

  Fragments arranged by size: 

                 631     546     524
 
Bpu10I CC'TnA_GC

Cuts at:      0     112     602    1701 
   Size:        112     490    1099

  Fragments arranged by size: 

                1099     490     112
 
BsaAI yAC'GTr

Cuts at:      0     597    1701 
   Size:        597    1104
 
BsaHI Gr'CG_yC

Cuts at:      0    1622    1701 
   Size:       1622      79
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     677     707    1701 
   Size:        677      30     994

  Fragments arranged by size: 

                 994     677      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     628    1315    1678    1701 
   Size:        628     687     363      23

  Fragments arranged by size: 

                 687     628     363      23
 
BseYI C'CCAG_C

Cuts at:      0      44     203     380     989    1701 
   Size:         44     159     177     609     712

  Fragments arranged by size: 

                 712     609     177     159      44
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289    1701 
   Size:        289    1412
 
BsiEI CG_ry'CG

Cuts at:      0     699     894    1022    1103    1311    1368    1563    1701 
   Size:        699     195     128      81     208      57     195     138

  Fragments arranged by size: 

                 699     208     195     195     138     128      81      57
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     486     596     773    1088    1274    1701 
   Size:        486     110     177     315     186     427

  Fragments arranged by size: 

                 486     427     315     186     177     110
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     447    1701 
   Size:        447    1254
 
Bsp1286I G_dGCh'C

Cuts at:      0      25      68     150     168     180     411     486     596 
   Size:         25      43      82      18      12     231      75     110

Cuts at:    596     641     773     799     988    1028    1077    1088    1176 
   Size:         45     132      26     189      40      49      11      88

Cuts at:   1176    1274    1701 
   Size:         98     427

  Fragments arranged by size: 

                 427     231     189     132     110      98      88      82
                  75      49      45      43      40      26      25      18
                  12      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     926    1219    1701 
   Size:        926     293     482

  Fragments arranged by size: 

                 926     482     293
 
BsrBI CCG'CTC

Cuts at:      0     783    1200    1284    1536    1701 
   Size:        783     417      84     252     165

  Fragments arranged by size: 

                 783     417     252     165      84
 
BsrFI r'CCGG_y

Cuts at:      0     230     845     859    1000    1050    1430    1444    1514 
   Size:        230     615      14     141      50     380      14      70

Cuts at:   1514    1701 
   Size:        187

  Fragments arranged by size: 

                 615     380     230     187     141      70      50      14
                  14
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     887    1701 
   Size:        887     814
 
BstEII G'GTnAC_C

Cuts at:      0     663    1701 
   Size:        663    1038
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      39     361     633    1701 
   Size:         39     322     272    1068

  Fragments arranged by size: 

                1068     322     272      39
 
BtgI C'CryG_G

Cuts at:      0      32     105     659    1016    1701 
   Size:         32      73     554     357     685

  Fragments arranged by size: 

                 685     554     357      73      32
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     154     417    1701 
   Size:        154     263    1284

  Fragments arranged by size: 

                1284     263     154
 
BtsI GCAGTG_nn'

Cuts at:      0     301    1701 
   Size:        301    1400
 
DraIII CAC_nnn'GTG

Cuts at:      0    1254    1701 
   Size:       1254     447
 
EaeI y'GGCC_r

Cuts at:      0      29     270     546     891    1019    1308    1644    1701 
   Size:         29     241     276     345     128     289     336      57

  Fragments arranged by size: 

                 345     336     289     276     241     128      57      29
            
 
EagI C'GGCC_G

Cuts at:      0     891    1019    1308    1701 
   Size:        891     128     289     393

  Fragments arranged by size: 

                 891     393     289     128
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1393    1701 
   Size:       1393     308
 
EcoICRI GAG'CTC

Cuts at:      0     484    1701 
   Size:        484    1217
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     631    1177    1470    1554    1701 
   Size:        631     546     293      84     147

  Fragments arranged by size: 

                 631     546     293     147      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      94     613    1701 
   Size:         94     519    1088

  Fragments arranged by size: 

                1088     519      94
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21      22      65     164     606     637     638    1073 
   Size:         21       1      43      99     442      31       1     435

Cuts at:   1073    1074    1095    1701 
   Size:          1      21     606

  Fragments arranged by size: 

                 606     442     435      99      43      31      21      21
                   1       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     765    1701 
   Size:        765     936
 
HaeII r_GCGC'y

Cuts at:      0     732     816    1164    1625    1701 
   Size:        732      84     348     461      76

  Fragments arranged by size: 

                 732     461     348      84      76
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     677     709    1701 
   Size:        677      32     992

  Fragments arranged by size: 

                 992     677      32
 
HincII GTy'rAC

Cuts at:      0     696    1701 
   Size:        696    1005
 
KasI G'GCGC_C

Cuts at:      0    1621    1701 
   Size:       1621      80
 
KpnI G_GTAC'C

Cuts at:      0    1042    1701 
   Size:       1042     659
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1523    1607    1701 
   Size:       1523      84      94

  Fragments arranged by size: 

                1523      94      84
 
MscI TGG'CCA

Cuts at:      0      31    1646    1701 
   Size:         31    1615      55

  Fragments arranged by size: 

                1615      55      31
 
MslI CAynn'nnrTG

Cuts at:      0     130     173     359    1701 
   Size:        130      43     186    1342

  Fragments arranged by size: 

                1342     186     130      43
 
MspA1I CmG'CkG

Cuts at:      0     107     418     661     993    1018    1127    1182    1701 
   Size:        107     311     243     332      25     109      55     519

  Fragments arranged by size: 

                 519     332     311     243     109     107      55      25
            
 
NaeI GCC'GGC

Cuts at:      0    1002    1701 
   Size:       1002     699
 
NarI GG'CG_CC

Cuts at:      0    1622    1701 
   Size:       1622      79
 
NcoI C'CATG_G

Cuts at:      0      32    1701 
   Size:         32    1669
 
NdeI CA'TA_TG

Cuts at:      0     532    1701 
   Size:        532    1169
 
NgoMIV G'CCGG_C

Cuts at:      0    1000    1701 
   Size:       1000     701
 
NotI GC'GGCC_GC

Cuts at:      0     891    1308    1701 
   Size:        891     417     393

  Fragments arranged by size: 

                 891     417     393
 
PasI CC'CwG_GG

Cuts at:      0      90     647    1323    1701 
   Size:         90     557     676     378

  Fragments arranged by size: 

                 676     557     378      90
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      90    1701 
   Size:         90    1611
 
PfoI T'CCnGG_A

Cuts at:      0    1613    1701 
   Size:       1613      88
 
PpuMI rG'GwC_Cy

Cuts at:      0    1095    1701 
   Size:       1095     606
 
PspOMI G'GGCC_C

Cuts at:      0      21      64     164     407     637    1073    1172    1701 
   Size:         21      43     100     243     230     436      99     529

  Fragments arranged by size: 

                 529     436     243     230     100      99      43      21
            
 
PvuI CG_AT'CG

Cuts at:      0    1103    1368    1701 
   Size:       1103     265     333

  Fragments arranged by size: 

                1103     333     265
 
PvuII CAG'CTG

Cuts at:      0     418     993    1127    1701 
   Size:        418     575     134     574

  Fragments arranged by size: 

                 575     574     418     134
 
SacI G_AGCT'C

Cuts at:      0     486    1701 
   Size:        486    1215
 
SacII CC_GC'GG

Cuts at:      0     108     662    1019    1701 
   Size:        108     554     357     682

  Fragments arranged by size: 

                 682     554     357     108
 
SalI G'TCGA_C

Cuts at:      0     694    1701 
   Size:        694    1007
 
SexAI A'CCwGG_T

Cuts at:      0    1250    1502    1701 
   Size:       1250     252     199

  Fragments arranged by size: 

                1250     252     199
 
SfcI C'TryA_G

Cuts at:      0    1122    1701 
   Size:       1122     579
 
SfoI GGC'GCC

Cuts at:      0    1623    1701 
   Size:       1623      78
 
SgrAI Cr'CCGG_yG

Cuts at:      0     230     845     859    1430    1444    1514    1701 
   Size:        230     615      14     571      14      70     187

  Fragments arranged by size: 

                 615     571     230     187      70      14      14
 
SmaI CCC'GGG

Cuts at:      0     980    1701 
   Size:        980     721
 
StuI AGG'CCT

Cuts at:      0    1399    1701 
   Size:       1399     302
 
StyI C'CwwG_G

Cuts at:      0      32     626    1701 
   Size:         32     594    1075

  Fragments arranged by size: 

                1075     594      32
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     195     372    1103    1609    1701 
   Size:        195     177     731     506      92

  Fragments arranged by size: 

                 731     506     195     177      92
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     931     963    1701 
   Size:        931      32     738

  Fragments arranged by size: 

                 931     738      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     336    1701 
   Size:        336    1365
 
XmaI C'CCGG_G

Cuts at:      0     978    1701 
   Size:        978     723
 
XmnI GAAnn'nnTTC

Cuts at:      0    1231    1701 
   Size:       1231     470
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI     AleI    AlwNI     ApaI
    ApaLI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI     BglI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BsaAI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrFI   BstAPI   BstEII    BstXI     BtgI    BtgZI     BtsI
   DraIII     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I   HincII     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI
     PasI    PflMI     PfoI    PpuMI   PspOMI     PvuI    PvuII     SacI
    SacII     SalI    SexAI     SfcI     SfoI    SgrAI     SmaI     StuI
     StyI    TaqII     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII   AflIII     AgeI     AhdI     AlfI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI     BmtI     BplI    BpuEI
     BsaI    BsaBI    BsaWI    BsiWI     BsmI    BspEI    BspHI    BsrDI
    BsrGI   BssHII    BssSI    BstBI    BstYI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI     DrdI     EarI    EcoRI    EcoRV     FseI     FspI
    FspAI  HindIII     HpaI     MfeI     MluI     NheI     NruI     NsiI
     NspI     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PstI    RsrII    SanDI     SapI     SbfI     ScaI
     SfiI     SgfI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI  Tth111I     XbaI     XhoI     ZraI
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