Restriction map for pF1KB5023
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# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbvCI       BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmFI       BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstF5I      BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        EagI        
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRV       FauI        
FbaI        FokI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MaeII       MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


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# ONE CUT ENZYMES (   39 enzymes) #
###################################

AhdI           53  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          420  GATCC                GGATC(4/5)
AlwNI         426  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI          53  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         413  GAGCTC               G[AT]GC[AT]^C
BbsI          704  GTCTTC               GAAGAC(2/6)
BcgI          781  GCAAAGGAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI          443  CTGGAG               CTGGAG(16/14)
BpuAI         704  GTCTTC               GAAGAC(2/6)
BseRI         476  GAGGAG               GAGGAG(10/8)
BsiHKAI       413  GAGCTC               G[AT]GC[AT]^C
BsiYI         889  CCTGTGATAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          889  CCTGTGATAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          389  GCATTC               GAATGC(1/-1)
BspHI         933  TCATGA               T^CATGA
BssKI         205  CCTGG                ^CC[ACGT]GG
BssSI         767  CACGAG               CACGAG(-5/-1)
BstAPI        310  GCAGAGTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         205  CCTGG                CC^[AT]GG
BstXI         126  CCATGCATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         493  GCATGC               GC[ACGT]^[ACGT]GC
DraI          367  TTTAAA               TTT^AAA
EaeI          923  TGGCCG               [CT]^GGCC[GA]
Eam1105I       53  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         889  CCTGTGATAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        205  CCTGG                ^CC[AT]GG
HaeIII        924  GGCC                 GG^CC
HincII        139  GTCAAC               GT[CT]^[GA]AC
HindII        139  GTCAAC               GT[CT]^[GA]AC
MvaI          205  CCTGG                CC^[AT]GG
NcoI          776  CCATGG               C^CATGG
NlaIV         577  GGAGCC               GG[ACGT]^[ACGT]CC
PshAI         697  GACTTGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          933  TCATGA               T^CATGA
SacI          413  GAGCTC               GAGCT^C
ScrFI         205  CCTGG                CC^[ACGT]GG
SfaNI         250  GCATC                GCATC(5/9)
SmlI          699  CTTGAG               C^T[CT][GA]AG
SphI          493  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI           43  GAATTT               [GA]^AATT[CT]
              832  GAATTT              
ApoI           43  GAATTT               [GA]^AATT[CT]
              832  GAATTT              
AvaII          52  GGACC                G^G[AT]CC
              123  GGTCC               
BanII         413  GAGCTC               G[GA]GC[CT]^C
              578  GAGCCC              
BfaI          284  CTAG                 C^TAG
              506  CTAG                
BsmAI         151  GAGAC                GTCTC(1/5)
              770  GAGAC               
Cfr13I         52  GGACC                G^G[ACGT]CC
              123  GGTCC               
DsaI          146  CCGTGG               C^C[GA][CT]GG
              776  CCATGG              
EcoT14I       776  CCATGG               C^C[AT][AT]GG
              824  CCAAGG              
EcoT22I       128  ATGCAT               ATGCA^T
              248  ATGCAT              
MaeI          284  CTAG                 C^TAG
              506  CTAG                
MboII         705  TCTTC                GAAGA(8/7)
              978  TCTTC               
MslI          127  CATGCATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              949  CATGGTAGTG          
NsiI          128  ATGCAT               ATGCA^T
              248  ATGCAT              
NspI          130  GCATGT               [GA]CATG^[CT]
              493  GCATGC              
PleI          702  GAGTC                GAGTC(4/5)
              787  GAGTC               
Sau96I         52  GGACC                G^G[ACGT]CC
              123  GGTCC               
StyI          776  CCATGG               C^C[AT][AT]GG
              824  CCAAGG              
TaqI          411  TCGA                 T^CGA
              790  TCGA                
TfiI           93  GATTC                G^A[AT]TC
              975  GATTC               
Tsp45I        269  GTGAC                ^GT[GC]AC
              711  GTCAC               
TthHB8I       411  TCGA                 T^CGA
              790  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AfaI        AluI        BbvI        BmyI        BsaJI       
Bsp1286I    BsrI        DdeI        DpnII       Fnu4HI      
HinfI       HphI        ItaI        MaeIII      MboI        
MnlI        MseI        MwoI        NdeII       NlaIII      
RsaI        Sau3AI      Tru9I       TseI        Tsp509I     
TspRI       
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