Restriction map for pF1KB5023
 (Linear) (Six-base) MAPSORT of: KIBB5023.seq  Check: 1769  from: 1  to: 987



KIBB5023.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AhdI GACnn_n'nnGTC

Cuts at:      0      58     987 
   Size:         58     929
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     437     469     987 
   Size:        437      32     518

  Fragments arranged by size: 

                 518     437      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     431     987 
   Size:        431     556
 
ApoI r'AATT_y

Cuts at:      0      43     832     987 
   Size:         43     789     155

  Fragments arranged by size: 

                 789     155      43
 
BanII G_rGCy'C

Cuts at:      0     417     582     987 
   Size:        417     165     405

  Fragments arranged by size: 

                 417     405     165
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     697     987 
   Size:        697     290
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     770     804     987 
   Size:        770      34     183

  Fragments arranged by size: 

                 770     183      34
 
BfrBI ATG'CAT

Cuts at:      0     130     250     987 
   Size:        130     120     737

  Fragments arranged by size: 

                 737     130     120
 
Bme1580I G_kGCm'C

Cuts at:      0     535     987 
   Size:        535     452
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     826     987 
   Size:        826     161
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     464     987 
   Size:        464     523
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     720     987 
   Size:        720     267
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     437     467     710     740     987 
   Size:        437      30     243      30     247

  Fragments arranged by size: 

                 437     247     243      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     491     987 
   Size:        491     496
 
BseYI C'CCAG_C

Cuts at:      0     220     987 
   Size:        220     767
 
BsiHKAI G_wGCw'C

Cuts at:      0     417     987 
   Size:        417     570
 
BsmI GAATG_Cn'

Cuts at:      0     389     987 
   Size:        389     598
 
Bsp1286I G_dGCh'C

Cuts at:      0     417     535     582     987 
   Size:        417     118      47     405

  Fragments arranged by size: 

                 417     405     118      47
 
BspHI T'CATG_A

Cuts at:      0     933     987 
   Size:        933      54
 
BssSI C'ACGA_G

Cuts at:      0     767     987 
   Size:        767     220
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     316     987 
   Size:        316     671
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     133     987 
   Size:        133     854
 
BtgI C'CryG_G

Cuts at:      0     146     776     987 
   Size:        146     630     211

  Fragments arranged by size: 

                 630     211     146
 
BtsI GCAGTG_nn'

Cuts at:      0     533     987 
   Size:        533     454
 
DraI TTT'AAA

Cuts at:      0     369     987 
   Size:        369     618
 
EaeI y'GGCC_r

Cuts at:      0     923     987 
   Size:        923      64
 
EcoICRI GAG'CTC

Cuts at:      0     415     987 
   Size:        415     572
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     464     987 
   Size:        464     523
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     893     987 
   Size:        893      94
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      44      76     235     267     368     400     404     436 
   Size:         44      32     159      32     101      32       4      32

Cuts at:    436     885     917     987 
   Size:        449      32      70

  Fragments arranged by size: 

                 449     159     101      70      44      32      32      32
                  32      32       4
 
HincII GTy'rAC

Cuts at:      0     141     987 
   Size:        141     846
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     219     462     987 
   Size:        219     243     525

  Fragments arranged by size: 

                 525     243     219
 
MslI CAynn'nnrTG

Cuts at:      0     131     953     987 
   Size:        131     822      34

  Fragments arranged by size: 

                 822     131      34
 
NcoI C'CATG_G

Cuts at:      0     776     987 
   Size:        776     211
 
NsiI A_TGCA'T

Cuts at:      0     132     252     987 
   Size:        132     120     735

  Fragments arranged by size: 

                 735     132     120
 
NspI r_CATG'y

Cuts at:      0     134     497     987 
   Size:        134     363     490

  Fragments arranged by size: 

                 490     363     134
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     437     469     987 
   Size:        437      32     518

  Fragments arranged by size: 

                 518     437      32
 
PshAI GACnn'nnGTC

Cuts at:      0     701     987 
   Size:        701     286
 
SacI G_AGCT'C

Cuts at:      0     417     987 
   Size:        417     570
 
SmlI C'TyrA_G

Cuts at:      0     699     987 
   Size:        699     288
 
SphI G_CATG'C

Cuts at:      0     497     987 
   Size:        497     490
 
StyI C'CwwG_G

Cuts at:      0     776     824     987 
   Size:        776      48     163

  Fragments arranged by size: 

                 776     163      48
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     710     742     987 
   Size:        710      32     245

  Fragments arranged by size: 

                 710     245      32
 

 Enzymes that do cut:

     AhdI     AloI    AlwNI     ApoI    BanII     BbsI     BcgI    BfrBI
 Bme1580I     BplI     BpmI    BpuEI    BsaXI    BseRI    BseYI  BsiHKAI
     BsmI Bsp1286I    BspHI    BssSI   BstAPI    BstXI     BtgI     BtsI
     DraI     EaeI  EcoICRI  Eco57MI    EcoNI    Hin4I   HincII     MmeI
     MslI     NcoI     NsiI     NspI     PpiI    PshAI     SacI     SmlI
     SphI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI   DraIII     DrdI     EagI     EarI     EciI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI    PpuMI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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