Restriction map for pF1KB5008
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BciVI       BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BseRI       BsiEI       BsiWI       
BsmBI       BspDI       BspHI       BspLU11I    BsrDI       
BsrFI       BssSI       Bst1107I    BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DdeI        DraI        DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HaeII       HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MluNI       MscI        MseI        MspA1I      MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        Tru9I       Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccIII        347  TCCGGA               T^CCGGA
AluI          317  AGCT                 AG^CT
AlwI          299  GGATC                GGATC(4/5)
AlwNI         733  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       447  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          770  CCCGGG               C^[CT]CG[GA]G
BcgI          507  CGACTGCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          668  TGATCA               T^GATCA
BglI          172  GCCTTCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          182  CTCCAG               CTGGAG(16/14)
BsaAI         643  TACGTG               [CT]AC^GT[GA]
BsaI          225  GGTCTC               GGTCTC(1/5)
BsaWI         347  TCCGGA               [AT]^CCGG[AT]
BseAI         347  TCCGGA               T^CCGGA
BsmAI         226  GTCTC                GTCTC(1/5)
BsmI          280  GCATTC               GAATGC(1/-1)
BsoBI         770  CCCGGG               C^[CT]CG[GA]G
BspEI         347  TCCGGA               T^CCGGA
BsrBI         142  GAGCGG               CCGCTC(-3/-3)
BsrI          732  CCAGT                ACTGG(1/-1)
BstAPI        854  GCACGGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         383  TTCGAA               TT^CGAA
BstYI         299  GGATCT               [GA]^GATC[CT]
DraIII        585  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          386  GAAGAG               CTCTTC(1/4)
EcoT14I       294  CCTTGG               C^C[AT][AT]GG
FbaI          668  TGATCA               T^GATCA
HincII        580  GTCAAC               GT[CT]^[GA]AC
HindII        580  GTCAAC               GT[CT]^[GA]AC
HphI          767  TCACC                GGTGA(8/7)
Ksp632I       386  GAAGAG               CTCTTC(1/4)
MaeII         644  ACGT                 A^CGT
MboII         386  GAAGA                GAAGA(8/7)
MflI          299  GGATCT               [GA]^GATC[CT]
MroI          347  TCCGGA               T^CCGGA
NspV          383  TTCGAA               TT^CGAA
PflMI         732  CCAGTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI           13  GACTC                GAGTC(4/5)
PstI          510  CTGCAG               CTGCA^G
SacI          316  GAGCTC               GAGCT^C
SfcI          510  CTGCAG               C^T[GA][CT]AG
SfuI          383  TTCGAA               TT^CGAA
SmaI          770  CCCGGG               CCC^GGG
SmlI           58  CTCAAG               C^T[CT][GA]AG
StyI          294  CCTTGG               C^C[AT][AT]GG
TfiI          495  GAATC                G^A[AT]TC
Tsp509I       393  AATT                 ^AATT
Van91I        732  CCAGTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          830  CCAAGCCCAGCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         299  GGATCT               [GA]^GATC[CT]
XmaCI         770  CCCGGG               C^CCGGG
XmaI          770  CCCGGG               C^CCGGG
XmnI          447  GAAAAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcyI          208  GGCGTC               G[GA]^CG[CT]C
              246  GGCGTC              
ApaI          104  GGGCCC               GGGCC^C
              820  GGGCCC              
AspHI         316  GAGCTC               G[AT]GC[AT]^C
              793  GAGCAC              
AvaII         111  GGTCC                G^G[AT]CC
              523  GGACC               
BanI          540  GGTGCC               G^G[CT][GA]CC
              826  GGTGCC              
BsaHI         208  GGCGTC               G[GA]^CG[CT]C
              246  GGCGTC              
BsgI          654  CTGCAC               GTGCAG(16/14)
              628  GTGCAG              
BsiHKAI       316  GAGCTC               G[AT]GC[AT]^C
              793  GAGCAC              
BsmFI          11  GGGAC                GGGAC(10/14)
              249  GTCCC               
Bsp1407I      287  TGTACA               T^GTACA
              761  TGTACA              
BspMI         550  ACCTGC               ACCTGC(4/8)
               21  GCAGGT              
BsrGI         287  TGTACA               T^GTACA
              761  TGTACA              
BssHII         35  GCGCGC               G^CGCGC
               37  GCGCGC              
Hin1I         208  GGCGTC               G[GA]^CG[CT]C
              246  GGCGTC              
HinfI          13  GACTC                G^A[ACGT]TC
              495  GAATC               
MaeIII        257  GTGAC                ^GT[ACGT]AC
              802  GTCAC               
SspBI         287  TGTACA               T^GTACA
              761  TGTACA              
TaqI          384  TCGA                 T^CGA
              608  TCGA                
Tsp45I        257  GTGAC                ^GT[GC]AC
              802  GTCAC               
TthHB8I       384  TCGA                 T^CGA
              608  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AccII       AciI        AfaI        BanII       BbvI        
BcnI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DpnII       DraII       
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HpaII       ItaI        MboI        MnlI        MslI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TseI        TspRI       
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