Restriction map for pF1KB5008
 (Linear) (Six-base) MAPSORT of: KIBB5008.seq  Check: 5367  from: 1  to: 882



KIBB5008.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      12     559     882 
   Size:         12     547     323

  Fragments arranged by size: 

                 547     323      12
 
AleI CACnn'nnGTG

Cuts at:      0     772     882 
   Size:        772     110
 
AlwNI CAG_nnn'CTG

Cuts at:      0     738     882 
   Size:        738     144
 
ApaI G_GGCC'C

Cuts at:      0     108     824     882 
   Size:        108     716      58

  Fragments arranged by size: 

                 716     108      58
 
AvaI C'yCGr_G

Cuts at:      0     770     882 
   Size:        770     112
 
BanI G'GyrC_C

Cuts at:      0     540     826     882 
   Size:        540     286      56

  Fragments arranged by size: 

                 540     286      56
 
BanII G_rGCy'C

Cuts at:      0     108     320     815     824     882 
   Size:        108     212     495       9      58

  Fragments arranged by size: 

                 495     212     108      58       9
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     496     530     882 
   Size:        496      34     352

  Fragments arranged by size: 

                 496     352      34
 
BclI T'GATC_A

Cuts at:      0     668     882 
   Size:        668     214
 
BglI GCCn_nnn'nGGC

Cuts at:      0     178     882 
   Size:        178     704
 
Bme1580I G_kGCm'C

Cuts at:      0     108     519     545     824     882 
   Size:        108     411      26     279      58

  Fragments arranged by size: 

                 411     279     108      58      26
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     726     882 
   Size:        726     156
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     882 
   Size:        167     715
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     882 
   Size:         43     839
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     231     882 
   Size:        231     651
 
BsaAI yAC'GTr

Cuts at:      0     645     882 
   Size:        645     237
 
BsaHI Gr'CG_yC

Cuts at:      0     209     247     882 
   Size:        209      38     635

  Fragments arranged by size: 

                 635     209      38
 
BsaWI w'CCGG_w

Cuts at:      0     347     882 
   Size:        347     535
 
BseYI C'CCAG_C

Cuts at:      0     835     882 
   Size:        835      47
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     639     649     882 
   Size:        639      10     233

  Fragments arranged by size: 

                 639     233      10
 
BsiHKAI G_wGCw'C

Cuts at:      0     320     797     882 
   Size:        320     477      85

  Fragments arranged by size: 

                 477     320      85
 
BsmI GAATG_Cn'

Cuts at:      0     280     882 
   Size:        280     602
 
Bsp1286I G_dGCh'C

Cuts at:      0     108     320     519     545     797     815     824     882 
   Size:        108     212     199      26     252      18       9      58

  Fragments arranged by size: 

                 252     212     199     108      58      26      18       9
            
 
BspEI T'CCGG_A

Cuts at:      0     347     882 
   Size:        347     535
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      12     559     882 
   Size:         12     547     323

  Fragments arranged by size: 

                 547     323      12
 
BsrBI CCG'CTC

Cuts at:      0     144     882 
   Size:        144     738
 
BsrGI T'GTAC_A

Cuts at:      0     287     761     882 
   Size:        287     474     121

  Fragments arranged by size: 

                 474     287     121
 
BssHII G'CGCG_C

Cuts at:      0      35      37     882 
   Size:         35       2     845

  Fragments arranged by size: 

                 845      35       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     860     882 
   Size:        860      22
 
BstBI TT'CG_AA

Cuts at:      0     384     882 
   Size:        384     498
 
BstYI r'GATC_y

Cuts at:      0     299     882 
   Size:        299     583
 
BtsI GCAGTG_nn'

Cuts at:      0     519     882 
   Size:        519     363
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     444     479     882 
   Size:        444      35     403

  Fragments arranged by size: 

                 444     403      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     590     882 
   Size:        590     292
 
EarI CTCTTCn'nnn_

Cuts at:      0     381     882 
   Size:        381     501
 
EcoICRI GAG'CTC

Cuts at:      0     318     882 
   Size:        318     564
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     882 
   Size:        167     715
 
EcoO109I rG'GnC_Cy

Cuts at:      0     104     105     820     882 
   Size:        104       1     715      62

  Fragments arranged by size: 

                 715     104      62       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     480     512     882 
   Size:        480      32     370

  Fragments arranged by size: 

                 480     370      32
 
HincII GTy'rAC

Cuts at:      0     582     882 
   Size:        582     300
 
MslI CAynn'nnrTG

Cuts at:      0     220     238     643     697     772     787     882 
   Size:        220      18     405      54      75      15      95

  Fragments arranged by size: 

                 405     220      95      75      54      18      15
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     738     882 
   Size:        738     144
 
PspOMI G'GGCC_C

Cuts at:      0     104     820     882 
   Size:        104     716      62

  Fragments arranged by size: 

                 716     104      62
 
PstI C_TGCA'G

Cuts at:      0     514     882 
   Size:        514     368
 
SacI G_AGCT'C

Cuts at:      0     320     882 
   Size:        320     562
 
SfcI C'TryA_G

Cuts at:      0     510     882 
   Size:        510     372
 
SmaI CCC'GGG

Cuts at:      0     772     882 
   Size:        772     110
 
SmlI C'TyrA_G

Cuts at:      0      58     882 
   Size:         58     824
 
StyI C'CwwG_G

Cuts at:      0     294     882 
   Size:        294     588
 
TatI w'GTAC_w

Cuts at:      0     287     761     882 
   Size:        287     474     121

  Fragments arranged by size: 

                 474     287     121
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     837     882 
   Size:        837      45
 
XmaI C'CCGG_G

Cuts at:      0     770     882 
   Size:        770     112
 
XmnI GAAnn'nnTTC

Cuts at:      0     451     882 
   Size:        451     431
 

 Enzymes that do cut:

     AarI     AleI    AlwNI     ApaI     AvaI     BanI    BanII     BcgI
     BclI     BglI Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspMI    BsrBI    BsrGI   BssHII   BstAPI    BstBI    BstYI     BtsI
    CspCI   DraIII     EarI  EcoICRI  Eco57MI EcoO109I    Hin4I   HincII
     MslI    PflMI   PspOMI     PstI     SacI     SfcI     SmaI     SmlI
     StyI     TatI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI
    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BsaBI
    BsaXI    BseRI    BsiEI    BsiWI    BsmBI    BspHI    BsrDI    BsrFI
    BssSI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
     DraI     DrdI     EaeI     EagI     EciI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     ZraI
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