Restriction map for pF1KB4987
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AhdI        Alw44I      AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvrII       BalI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaI        BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       FbaI        
FseI        HincII      HindII      HindIII     HpaI        
KpnI        MaeI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PflFI       PflMI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SalI        SapI        ScaI        
SexAI       SfiI        SfuI        SmaI        SnaBI       
SpeI        SspI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII         964  GACGTC               GACGT^C
AccII         159  CGCG                 CG^CG
AflIII       1190  ACGTGT               A^C[GA][CT]GT
AgeI         1279  ACCGGT               A^CCGGT
ApaI          486  GGGCCC               GGGCC^C
Asp700I      1371  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         395  TGCGCA               TGC^GCA
BamHI         939  GGATCC               G^GATCC
BanI         1033  GGCGCC               G^G[CT][GA]CC
BbeI         1033  GGCGCC               GGCGC^C
BglI          844  GCCATCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         884  AGATCT               A^GATCT
BsaBI         424  GATATGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1279  ACCGGT               [AT]^CCGG[AT]
BsmI          651  GAATGC               GAATGC(1/-1)
Bsp1407I      473  TGTACA               T^GTACA
BspHI        1379  TCATGA               T^CATGA
BsrDI         502  GCAATG               GCAATG(2/0)
BsrGI         473  TGTACA               T^GTACA
BstEII        514  GGTCACC              G^GT[ACGT]ACC
BstPI         514  GGTCACC              G^GT[ACGT]ACC
BstUI         159  CGCG                 CG^CG
DdeI          732  CTCAG                C^T[ACGT]AG
DraIII        537  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         621  CCTGGACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       514  GGTCACC              G^GT[ACGT]ACC
EcoRV         904  GATATC               GAT^ATC
EcoT22I       505  ATGCAT               ATGCA^T
FspI          395  TGCGCA               TGC^GCA
HaeII        1033  GGCGCC               [GA]GCGC^[CT]
HgaI           91  GCGTC                GACGC(5/10)
KasI         1033  GGCGCC               G^GCGCC
KspI          158  CCGCGG               CCGC^GG
MamI          424  GATATGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI          159  CGCG                 CG^CG
NarI         1033  GGCGCC               GG^CGCC
NcoI          259  CCATGG               C^CATGG
NsiI          505  ATGCAT               ATGCA^T
PinAI        1279  ACCGGT               A^CCGGT
PleI         1383  GACTC                GAGTC(4/5)
RcaI         1379  TCATGA               T^CATGA
SacII         158  CCGCGG               CCGC^GG
SbfI         1026  CCTGCAGG             CCTGCA^GG
SfoI         1033  GGCGCC               GGC^GCC
SgrAI        1278  CACCGGTG             C[GA]^CCGG[CT]G
SphI         1122  GCATGC               GCATG^C
Sse8387I     1026  CCTGCAGG             CCTGCA^GG
SspBI         473  TGTACA               T^GTACA
StuI          629  AGGCCT               AGG^CCT
XmnI         1371  GAAAGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcyI          964  GACGTC               G[GA]^CG[CT]C
             1033  GGCGCC              
AlwNI         233  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              813  CAGCGACTG           
AvaI         1219  CTCGAG               C^[CT]CG[GA]G
             1237  CTCGAG              
BsaHI         964  GACGTC               G[GA]^CG[CT]C
             1033  GGCGCC              
BseRI        1068  CTCCTC               GAGGAG(10/8)
             1153  GAGGAG              
BsmAI         893  GAGAC                GTCTC(1/5)
             1142  GAGAC               
BsmFI         962  GGGAC                GGGAC(10/14)
               72  GTCCC               
BsoBI        1219  CTCGAG               C^[CT]CG[GA]G
             1237  CTCGAG              
BsrFI         370  ACCGGC               [GA]^CCGG[CT]
             1279  ACCGGT              
BsrI          209  CCAGT                ACTGG(1/-1)
              909  CCAGT               
BstYI         884  AGATCT               [GA]^GATC[CT]
              939  GGATCC              
Cfr10I        370  ACCGGC               [GA]^CCGG[CT]
             1279  ACCGGT              
DsaI          158  CCGCGG               C^C[GA][CT]GG
              259  CCATGG              
EaeI          372  CGGCCA               [CT]^GGCC[GA]
             1091  CGGCCA              
EarI          492  CTCTTC               CTCTTC(1/4)
              241  GAAGAG              
Hin1I         964  GACGTC               G[GA]^CG[CT]C
             1033  GGCGCC              
Ksp632I       492  CTCTTC               CTCTTC(1/4)
              241  GAAGAG              
MflI          884  AGATCT               [GA]^GATC[CT]
              939  GGATCC              
MseI          136  TTAA                 T^TAA
              560  TTAA                
MslI          576  CATCCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              720  CATGGGGATG          
MspA1I        158  CCGCGG               C[AC]G^C[GT]G
             1263  CAGCGG              
PaeR7I       1219  CTCGAG               C^TCGAG
             1237  CTCGAG              
PpuMI          70  AGGTCCC              [GA]G^G[AT]CC[CT]
              611  AGGACCT             
Tru9I         136  TTAA                 T^TAA
              560  TTAA                
Tsp45I        515  GTCAC                ^GT[GC]AC
             1354  GTCAC               
Tsp509I       105  AATT                 ^AATT
              333  AATT                
XhoI         1219  CTCGAG               C^TCGAG
             1237  CTCGAG              
XhoII         884  AGATCT               [GA]^GATC[CT]
              939  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BsiHKAI     BsiYI       BslI        
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DpnII       DraII       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PstI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        TseI        TspRI       
TthHB8I     
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