Restriction map for pF1KB4987
 (Linear) (Six-base) MAPSORT of: KIBB4987.seq  Check: 9352  from: 1  to: 1413



KIBB4987.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AatII G_ACGT'C

Cuts at:      0     968    1413 
   Size:        968     445
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     518     849    1413 
   Size:        260     258     331     564

  Fragments arranged by size: 

                 564     331     260     258
 
AflIII A'CryG_T

Cuts at:      0    1190    1413 
   Size:       1190     223
 
AgeI A'CCGG_T

Cuts at:      0    1279    1413 
   Size:       1279     134
 
AlwNI CAG_nnn'CTG

Cuts at:      0     238     818    1413 
   Size:        238     580     595

  Fragments arranged by size: 

                 595     580     238
 
ApaI G_GGCC'C

Cuts at:      0     490    1413 
   Size:        490     923
 
AvaI C'yCGr_G

Cuts at:      0    1219    1237    1413 
   Size:       1219      18     176

  Fragments arranged by size: 

                1219     176      18
 
BamHI G'GATC_C

Cuts at:      0     939    1413 
   Size:        939     474
 
BanI G'GyrC_C

Cuts at:      0    1033    1413 
   Size:       1033     380
 
BanII G_rGCy'C

Cuts at:      0      17      65     490     733    1289    1413 
   Size:         17      48     425     243     556     124

  Fragments arranged by size: 

                 556     425     243     124      48      17
 
BbeI G_GCGC'C

Cuts at:      0    1037    1413 
   Size:       1037     376
 
BfrBI ATG'CAT

Cuts at:      0     507    1413 
   Size:        507     906
 
BglI GCCn_nnn'nGGC

Cuts at:      0     850    1413 
   Size:        850     563
 
BglII A'GATC_T

Cuts at:      0     884    1413 
   Size:        884     529
 
Bme1580I G_kGCm'C

Cuts at:      0     490     569    1413 
   Size:        490      79     844

  Fragments arranged by size: 

                 844     490      79
 
BmgBI CAC'GTC

Cuts at:      0    1191    1413 
   Size:       1191     222
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1291    1413 
   Size:       1291     122
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     173     532    1154    1413 
   Size:         49     124     359     622     259

  Fragments arranged by size: 

                 622     359     259     124      49
 
Bpu10I CC'TnA_GC

Cuts at:      0     732    1413 
   Size:        732     681
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1273    1413 
   Size:       1273     140
 
BsaBI GATnn'nnATC

Cuts at:      0     428    1413 
   Size:        428     985
 
BsaHI Gr'CG_yC

Cuts at:      0     965    1034    1413 
   Size:        965      69     379

  Fragments arranged by size: 

                 965     379      69
 
BsaWI w'CCGG_w

Cuts at:      0    1279    1413 
   Size:       1279     134
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1051    1081    1413 
   Size:       1051      30     332

  Fragments arranged by size: 

                1051     332      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1059    1168    1413 
   Size:       1059     109     245

  Fragments arranged by size: 

                1059     245     109
 
BseYI C'CCAG_C

Cuts at:      0     198    1020    1413 
   Size:        198     822     393

  Fragments arranged by size: 

                 822     393     198
 
BsiHKAI G_wGCw'C

Cuts at:      0      65     733    1160    1289    1334    1413 
   Size:         65     668     427     129      45      79

  Fragments arranged by size: 

                 668     427     129      79      65      45
 
BsmI GAATG_Cn'

Cuts at:      0     657    1413 
   Size:        657     756
 
Bsp1286I G_dGCh'C

Cuts at:      0      17      65     490     569     733    1160    1289    1334 
   Size:         17      48     425      79     164     427     129      45

Cuts at:   1334    1413 
   Size:         79

  Fragments arranged by size: 

                 427     425     164     129      79      79      48      45
                  17
 
BspHI T'CATG_A

Cuts at:      0    1379    1413 
   Size:       1379      34
 
BsrDI GCAATG_nn'

Cuts at:      0     509    1413 
   Size:        509     904
 
BsrFI r'CCGG_y

Cuts at:      0     370    1279    1413 
   Size:        370     909     134

  Fragments arranged by size: 

                 909     370     134
 
BsrGI T'GTAC_A

Cuts at:      0     473    1413 
   Size:        473     940
 
BstEII G'GTnAC_C

Cuts at:      0     514    1413 
   Size:        514     899
 
BstYI r'GATC_y

Cuts at:      0     884     939    1413 
   Size:        884      55     474

  Fragments arranged by size: 

                 884     474      55
 
BtgI C'CryG_G

Cuts at:      0     158     259    1413 
   Size:        158     101    1154

  Fragments arranged by size: 

                1154     158     101
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      30     651    1413 
   Size:         30     621     762

  Fragments arranged by size: 

                 762     621      30
 
BtsI GCAGTG_nn'

Cuts at:      0     285    1413 
   Size:        285    1128
 
DraIII CAC_nnn'GTG

Cuts at:      0     542    1413 
   Size:        542     871
 
EaeI y'GGCC_r

Cuts at:      0     372    1091    1413 
   Size:        372     719     322

  Fragments arranged by size: 

                 719     372     322
 
EarI CTCTTCn'nnn_

Cuts at:      0     236     498    1413 
   Size:        236     262     915

  Fragments arranged by size: 

                 915     262     236
 
EcoICRI GAG'CTC

Cuts at:      0      63     731    1287    1413 
   Size:         63     668     556     126

  Fragments arranged by size: 

                 668     556     126      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     173     260     518     532     849    1154    1413 
   Size:         49     124      87     258      14     317     305     259

  Fragments arranged by size: 

                 317     305     259     258     124      87      49      14
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     625    1413 
   Size:        625     788
 
EcoO109I rG'GnC_Cy

Cuts at:      0      71     486     487     612    1413 
   Size:         71     415       1     125     801

  Fragments arranged by size: 

                 801     415     125      71       1
 
EcoRV GAT'ATC

Cuts at:      0     906    1413 
   Size:        906     507
 
FspI TGC'GCA

Cuts at:      0     397    1413 
   Size:        397    1016
 
HaeII r_GCGC'y

Cuts at:      0    1037    1413 
   Size:       1037     376
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       4      36     969    1001    1051    1083    1133    1165 
   Size:          4      32     933      32      50      32      50      32

Cuts at:   1165    1413 
   Size:        248

  Fragments arranged by size: 

                 933     248      50      50      32      32      32      32
                   4
 
KasI G'GCGC_C

Cuts at:      0    1033    1413 
   Size:       1033     380
 
MslI CAynn'nnrTG

Cuts at:      0     580     724    1413 
   Size:        580     144     689

  Fragments arranged by size: 

                 689     580     144
 
MspA1I CmG'CkG

Cuts at:      0     160    1265    1413 
   Size:        160    1105     148

  Fragments arranged by size: 

                1105     160     148
 
NarI GG'CG_CC

Cuts at:      0    1034    1413 
   Size:       1034     379
 
NcoI C'CATG_G

Cuts at:      0     259    1413 
   Size:        259    1154
 
NsiI A_TGCA'T

Cuts at:      0     509    1413 
   Size:        509     904
 
NspI r_CATG'y

Cuts at:      0     511     772    1126    1413 
   Size:        511     261     354     287

  Fragments arranged by size: 

                 511     354     287     261
 
PfoI T'CCnGG_A

Cuts at:      0     548    1413 
   Size:        548     865
 
PpuMI rG'GwC_Cy

Cuts at:      0      71     612    1413 
   Size:         71     541     801

  Fragments arranged by size: 

                 801     541      71
 
PspOMI G'GGCC_C

Cuts at:      0     486    1413 
   Size:        486     927
 
PspXI vC'TCGA_Gb

Cuts at:      0    1219    1237    1413 
   Size:       1219      18     176

  Fragments arranged by size: 

                1219     176      18
 
PstI C_TGCA'G

Cuts at:      0     117    1031    1316    1413 
   Size:        117     914     285      97

  Fragments arranged by size: 

                 914     285     117      97
 
SacI G_AGCT'C

Cuts at:      0      65     733    1289    1413 
   Size:         65     668     556     124

  Fragments arranged by size: 

                 668     556     124      65
 
SacII CC_GC'GG

Cuts at:      0     161    1413 
   Size:        161    1252
 
SbfI CC_TGCA'GG

Cuts at:      0    1031    1413 
   Size:       1031     382
 
SfcI C'TryA_G

Cuts at:      0     113     633    1027    1312    1413 
   Size:        113     520     394     285     101

  Fragments arranged by size: 

                 520     394     285     113     101
 
SfoI GGC'GCC

Cuts at:      0    1035    1413 
   Size:       1035     378
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1279    1413 
   Size:       1279     134
 
SmlI C'TyrA_G

Cuts at:      0    1219    1237    1288    1413 
   Size:       1219      18      51     125

  Fragments arranged by size: 

                1219     125      51      18
 
SphI G_CATG'C

Cuts at:      0    1126    1413 
   Size:       1126     287
 
StuI AGG'CCT

Cuts at:      0     631    1413 
   Size:        631     782
 
StyI C'CwwG_G

Cuts at:      0     259     569     656    1109    1413 
   Size:        259     310      87     453     304

  Fragments arranged by size: 

                 453     310     304     259      87
 
TatI w'GTAC_w

Cuts at:      0     267     473    1413 
   Size:        267     206     940

  Fragments arranged by size: 

                 940     267     206
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     374     406     872     895     904     927    1413 
   Size:        374      32     466      23       9      23     486

  Fragments arranged by size: 

                 486     466     374      32      23      23       9
 
XhoI C'TCGA_G

Cuts at:      0    1219    1237    1413 
   Size:       1219      18     176

  Fragments arranged by size: 

                1219     176      18
 
XmnI GAAnn'nnTTC

Cuts at:      0    1375    1413 
   Size:       1375      38
 
ZraI GAC'GTC

Cuts at:      0     966    1413 
   Size:        966     447
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AgeI    AlwNI     ApaI     AvaI    BamHI
     BanI    BanII     BbeI    BfrBI     BglI    BglII Bme1580I    BmgBI
     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI    BsrFI
    BsrGI   BstEII    BstYI     BtgI    BtgZI     BtsI   DraIII     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FspI    HaeII
    Hin4I     KasI     MslI   MspA1I     NarI     NcoI     NsiI     NspI
     PfoI    PpuMI   PspOMI    PspXI     PstI     SacI    SacII     SbfI
     SfcI     SfoI    SgrAI     SmlI     SphI     StuI     StyI     TatI
     TstI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AhdI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbsI    BbvCI     BcgI    BciVI     BclI     BlpI     BmrI
     BmtI     BsaI    BsaAI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspMI    BsrBI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI     DrdI     EagI     EciI    EcoRI
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI     PsrI     PvuI    PvuII    RsrII     SalI    SanDI
     SapI     ScaI    SexAI     SfiI     SgfI     SmaI    SnaBI     SpeI
     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI     XcmI     XmaI
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