Restriction map for pF1KB4697
 (Linear) (Six-base) MAPSORT of: KIBB4697.seq  Check: 1117  from: 1  to: 981



KIBB4697.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     894     981 
   Size:        894      87
 
AccI GT'mk_AC

Cuts at:      0     473     981 
   Size:        473     508
 
Acc65I G'GTAC_C

Cuts at:      0     710     981 
   Size:        710     271
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     808     952     981 
   Size:        808     144      29

  Fragments arranged by size: 

                 808     144      29
 
AflII C'TTAA_G

Cuts at:      0     664     981 
   Size:        664     317
 
AflIII A'CryG_T

Cuts at:      0     770     981 
   Size:        770     211
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     364     981 
   Size:        364     617
 
AlwNI CAG_nnn'CTG

Cuts at:      0      30     930     981 
   Size:         30     900      51

  Fragments arranged by size: 

                 900      51      30
 
ApaI G_GGCC'C

Cuts at:      0     155     981 
   Size:        155     826
 
ApoI r'AATT_y

Cuts at:      0     658     981 
   Size:        658     323
 
AvaI C'yCGr_G

Cuts at:      0     498     726     981 
   Size:        498     228     255

  Fragments arranged by size: 

                 498     255     228
 
BanI G'GyrC_C

Cuts at:      0      85     127     710     981 
   Size:         85      42     583     271

  Fragments arranged by size: 

                 583     271      85      42
 
BanII G_rGCy'C

Cuts at:      0     155     567     848     981 
   Size:        155     412     281     133

  Fragments arranged by size: 

                 412     281     155     133
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     318     524     665     981 
   Size:        318     206     141     316

  Fragments arranged by size: 

                 318     316     206     141
 
BbvCI CC'TCA_GC

Cuts at:      0     337     981 
   Size:        337     644
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     414     448     981 
   Size:        414      34     533

  Fragments arranged by size: 

                 533     414      34
 
Bme1580I G_kGCm'C

Cuts at:      0     155     236     981 
   Size:        155      81     745

  Fragments arranged by size: 

                 745     155      81
 
BmgBI CAC'GTC

Cuts at:      0     771     948     981 
   Size:        771     177      33

  Fragments arranged by size: 

                 771     177      33
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     303     747     981 
   Size:         90     213     444     234

  Fragments arranged by size: 

                 444     234     213      90
 
Bpu10I CC'TnA_GC

Cuts at:      0     337     981 
   Size:        337     644
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     723     835     981 
   Size:        723     112     146

  Fragments arranged by size: 

                 723     146     112
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     580     981 
   Size:        580     401
 
BsaHI Gr'CG_yC

Cuts at:      0     596     670     981 
   Size:        596      74     311

  Fragments arranged by size: 

                 596     311      74
 
BsaWI w'CCGG_w

Cuts at:      0     242     981 
   Size:        242     739
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     289     981 
   Size:        289     692
 
BseYI C'CCAG_C

Cuts at:      0     357     981 
   Size:        357     624
 
BsiEI CG_ry'CG

Cuts at:      0      15      44     981 
   Size:         15      29     937

  Fragments arranged by size: 

                 937      29      15
 
BsiHKAI G_wGCw'C

Cuts at:      0     567     981 
   Size:        567     414
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     236     567     848     981 
   Size:        155      81     331     281     133

  Fragments arranged by size: 

                 331     281     155     133      81
 
BspEI T'CCGG_A

Cuts at:      0     242     981 
   Size:        242     739
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     894     981 
   Size:        894      87
 
BsrFI r'CCGG_y

Cuts at:      0     421     713     981 
   Size:        421     292     268

  Fragments arranged by size: 

                 421     292     268
 
BsrGI T'GTAC_A

Cuts at:      0     827     981 
   Size:        827     154
 
BssHII G'CGCG_C

Cuts at:      0     110     981 
   Size:        110     871
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      30     981 
   Size:         30     951
 
BstEII G'GTnAC_C

Cuts at:      0     493     855     981 
   Size:        493     362     126

  Fragments arranged by size: 

                 493     362     126
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     810     981 
   Size:        810     171
 
BstYI r'GATC_y

Cuts at:      0     221     386     981 
   Size:        221     165     595

  Fragments arranged by size: 

                 595     221     165
 
BtgI C'CryG_G

Cuts at:      0      15      38     140     171     590     779     809     981 
   Size:         15      23     102      31     419     189      30     172

  Fragments arranged by size: 

                 419     189     172     102      31      30      23      15
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      21      60     981 
   Size:         21      39     921

  Fragments arranged by size: 

                 921      39      21
 
BtsI GCAGTG_nn'

Cuts at:      0     234     981 
   Size:        234     747
 
EaeI y'GGCC_r

Cuts at:      0      41      71     981 
   Size:         41      30     910

  Fragments arranged by size: 

                 910      41      30
 
EagI C'GGCC_G

Cuts at:      0      41     981 
   Size:         41     940
 
EarI CTCTTCn'nnn_

Cuts at:      0     436     684     981 
   Size:        436     248     297

  Fragments arranged by size: 

                 436     297     248
 
EcoICRI GAG'CTC

Cuts at:      0     565     981 
   Size:        565     416
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     303     747     808     952     981 
   Size:         90     213     444      61     144      29

  Fragments arranged by size: 

                 444     213     144      90      61      29
 
EcoO109I rG'GnC_Cy

Cuts at:      0     152     927     981 
   Size:        152     775      54

  Fragments arranged by size: 

                 775     152      54
 
EcoRI G'AATT_C

Cuts at:      0     658     981 
   Size:        658     323
 
HaeII r_GCGC'y

Cuts at:      0     726     981 
   Size:        726     255
 
KpnI G_GTAC'C

Cuts at:      0     714     981 
   Size:        714     267
 
MscI TGG'CCA

Cuts at:      0      73     981 
   Size:         73     908
 
MslI CAynn'nnrTG

Cuts at:      0     808     981 
   Size:        808     173
 
MspA1I CmG'CkG

Cuts at:      0      17     173     981 
   Size:         17     156     808

  Fragments arranged by size: 

                 808     156      17
 
NaeI GCC'GGC

Cuts at:      0     423     981 
   Size:        423     558
 
NcoI C'CATG_G

Cuts at:      0     140     779     809     981 
   Size:        140     639      30     172

  Fragments arranged by size: 

                 639     172     140      30
 
NgoMIV G'CCGG_C

Cuts at:      0     421     981 
   Size:        421     560
 
NspI r_CATG'y

Cuts at:      0     957     981 
   Size:        957      24
 
PpuMI rG'GwC_Cy

Cuts at:      0     927     981 
   Size:        927      54
 
PspOMI G'GGCC_C

Cuts at:      0     151     981 
   Size:        151     830
 
PspXI vC'TCGA_Gb

Cuts at:      0     726     981 
   Size:        726     255
 
PstI C_TGCA'G

Cuts at:      0      26     444     905     981 
   Size:         26     418     461      76

  Fragments arranged by size: 

                 461     418      76      26
 
SacI G_AGCT'C

Cuts at:      0     567     981 
   Size:        567     414
 
SacII CC_GC'GG

Cuts at:      0      18     174     981 
   Size:         18     156     807

  Fragments arranged by size: 

                 807     156      18
 
SbfI CC_TGCA'GG

Cuts at:      0     905     981 
   Size:        905      76
 
SfcI C'TryA_G

Cuts at:      0      22     390     440     705     873     901     981 
   Size:         22     368      50     265     168      28      80

  Fragments arranged by size: 

                 368     265     168      80      50      28      22
 
SmlI C'TyrA_G

Cuts at:      0     664     726     738     850     981 
   Size:        664      62      12     112     131

  Fragments arranged by size: 

                 664     131     112      62      12
 
SphI G_CATG'C

Cuts at:      0     957     981 
   Size:        957      24
 
StuI AGG'CCT

Cuts at:      0     446     754     981 
   Size:        446     308     227

  Fragments arranged by size: 

                 446     308     227
 
StyI C'CwwG_G

Cuts at:      0     140     779     809     981 
   Size:        140     639      30     172

  Fragments arranged by size: 

                 639     172     140      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     199     349     981 
   Size:        199     150     632

  Fragments arranged by size: 

                 632     199     150
 
TatI w'GTAC_w

Cuts at:      0     101     827     981 
   Size:        101     726     154

  Fragments arranged by size: 

                 726     154     101
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     138     981 
   Size:        138     843
 
XhoI C'TCGA_G

Cuts at:      0     726     981 
   Size:        726     255
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AflII   AflIII     AlfI    AlwNI
     ApaI     ApoI     AvaI     BanI    BanII     BbsI    BbvCI     BcgI
 Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrFI
    BsrGI   BssHII   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     EaeI     EagI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI
    HaeII     KpnI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NspI    PpuMI   PspOMI    PspXI     PstI     SacI    SacII     SbfI
     SfcI     SmlI     SphI     StuI     StyI    TaqII     TatI     XcmI
     XhoI

 Enzymes that do not cut: 

    AatII     AclI     AfeI     AgeI     AhdI     AleI     AloI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI
    BsaAI    BsaBI    BsaXI     BsgI    BsiWI     BsmI    BsmBI    BspHI
    BsrBI    BsrDI    BssSI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI
     DraI   DraIII     DrdI     EciI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     NarI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI     PsrI     PvuI    PvuII    RsrII     SalI    SanDI
     SapI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI
     XmaI     XmnI     ZraI
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