Restriction map for pF1KB4295
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BbeI        BbrPI       BbsI        
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BseAI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HphI        
KasI        Ksp632I     KspI        MaeI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StyI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        619  GGTACC               G^GTACC
Asp718I       619  GGTACC               G^GTACC
AspHI         256  GTGCTC               G[AT]GC[AT]^C
AvaII         668  GGACC                G^G[AT]CC
BamHI         537  GGATCC               G^GATCC
BanI          619  GGTACC               G^G[CT][GA]CC
BanII         242  GAGCCC               G[GA]GC[CT]^C
BbvI          695  GCAGC                GCAGC(8/12)
BglII         462  AGATCT               A^GATCT
BsaJI         245  CCCAGG               C^C[ACGT][ACGT]GG
BsaWI         604  TCCGGT               [AT]^CCGG[AT]
BseRI         239  GAGGAG               GAGGAG(10/8)
BsiHKAI       256  GTGCTC               G[AT]GC[AT]^C
BsiYI         272  CCACAGATAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          272  CCACAGATAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         188  GGGAC                GGGAC(10/14)
BsmI          552  GCATTC               GAATGC(1/-1)
BspMI         196  GCAGGT               ACCTGC(4/8)
BssKI         246  CCAGG                ^CC[ACGT]GG
BssSI         259  CTCGTG               CACGAG(-5/-1)
BstF5I        378  GGATG                GGATG(2/0)
BstNI         246  CCAGG                CC^[AT]GG
BstXI          26  CCATAAAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I        668  GGACC                G^G[ACGT]CC
EcoRI         612  GAATTC               G^AATTC
EcoRII        246  CCAGG                ^CC[AT]GG
Fnu4HI        695  GCAGC                GC^[ACGT]GC
FokI          378  GGATG                GGATG(9/13)
HaeIII        228  GGCC                 GG^CC
HapII         605  CCGG                 C^CGG
HpaII         605  CCGG                 C^CGG
ItaI          695  GCAGC                GC^[ACGT]GC
KpnI          619  GGTACC               GGTAC^C
MslI           27  CATAAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        696  CAGCTG               C[AC]G^C[GT]G
MspI          605  CCGG                 C^CGG
MvaI          246  CCAGG                CC^[AT]GG
MwoI          695  GCAGCTGTAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PstI          194  CTGCAG               CTGCA^G
PvuII         696  CAGCTG               CAG^CTG
Sau96I        668  GGACC                G^G[ACGT]CC
ScrFI         246  CCAGG                CC^[ACGT]GG
SfaNI         214  GATGC                GCATC(5/9)
StuI          227  AGGCCT               AGG^CCT
TaqI          237  TCGA                 T^CGA
TseI          695  GCAGC                G^C[AT]GC
Tsp45I        599  GTCAC                ^GT[GC]AC
TthHB8I       237  TCGA                 T^CGA
XcmI           26  CCATAAAGATGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AfaI          349  GTAC                 GT^AC
              620  GTAC                
AlwI          538  GATCC                GGATC(4/5)
              537  GGATC               
BbvCI         406  GCTGAGG              CCTCAGC(-5/-2)
              532  GCTGAGG             
BmyI          242  GAGCCC               G[AGT]GC[ACT]^C
              256  GTGCTC              
Bsp1286I      242  GAGCCC               G[AGT]GC[ACT]^C
              256  GTGCTC              
BsrI          414  ACTGG                ACTGG(1/-1)
              302  CCAGT               
BstYI         462  AGATCT               [GA]^GATC[CT]
              537  GGATCC              
MaeIII        563  GTAAC                ^GT[ACGT]AC
              599  GTCAC               
MflI          462  AGATCT               [GA]^GATC[CT]
              537  GGATCC              
RsaI          349  GTAC                 GT^AC
              620  GTAC                
XhoII         462  AGATCT               [GA]^GATC[CT]
              537  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AcsI        AluI        ApoI        DdeI        DpnII       
HinfI       MboI        MboII       MnlI        MseI        
NdeII       NlaIV       Sau3AI      SfcI        TfiI        
Tru9I       Tsp509I     TspRI       
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