Restriction map for
pF1KB4295
(Circular) (Six-base) MAPSORT of: pF1KB4295.seq Check: 8127 from: 1 to: 3824
pF1KB4295 3824 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 862 862
Size: 3824
Acc65I G'GTAC_C
Cuts at: 727 840 727
Size: 113 3711
AclI AA'CG_TT
Cuts at: 3664 3664
Size: 3824
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 1649 2081 2828 3096 601
Size: 1048 432 747 268 1329
Fragments arranged by size:
1329 1048 747 432 268
AflIII A'CryG_T
Cuts at: 1176 2280 3257 1176
Size: 1104 977 1743
Fragments arranged by size:
1743 1104 977
AgeI A'CCGG_T
Cuts at: 2212 2212
Size: 3824
AlwNI CAG_nnn'CTG
Cuts at: 1129 2696 1129
Size: 1567 2257
ApaLI G'TGCA_C
Cuts at: 2594 2594
Size: 3824
ApoI r'AATT_y
Cuts at: 257 316 720 828 3177 3433 257
Size: 59 404 108 2349 256 648
Fragments arranged by size:
2349 648 404 256 108 59
AseI AT'TA_AT
Cuts at: 20 20
Size: 3824
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3824
AvaI C'yCGr_G
Cuts at: 844 844
Size: 3824
BamHI G'GATC_C
Cuts at: 645 849 645
Size: 204 3620
BanI G'GyrC_C
Cuts at: 727 840 1254 1502 1537 727
Size: 113 414 248 35 3014
Fragments arranged by size:
3014 414 248 113 35
BanII G_rGCy'C
Cuts at: 354 838 1868 354
Size: 484 1030 2310
Fragments arranged by size:
2310 1030 484
BbeI G_GCGC'C
Cuts at: 1258 1506 1258
Size: 248 3576
BbvCI CC'TCA_GC
Cuts at: 515 641 515
Size: 126 3698
BciVI GTATCCnnnnn_n'
Cuts at: 1722 2483 3824 1722
Size: 761 1341 1722
Fragments arranged by size:
1722 1341 761
BclI T'GATC_A
Cuts at: 1344 1344
Size: 3824
BglII A'GATC_T
Cuts at: 570 1339 3376 570
Size: 769 2037 1018
Fragments arranged by size:
2037 1018 769
BlpI GC'TnA_GC
Cuts at: 936 936
Size: 3824
Bme1580I G_kGCm'C
Cuts at: 1449 1542 2598 1449
Size: 93 1056 2675
Fragments arranged by size:
2675 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1036 1117 1452 3596 1036
Size: 81 335 2144 1264
Fragments arranged by size:
2144 1264 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 3824
Bpu10I CC'TnA_GC
Cuts at: 515 641 515
Size: 126 3698
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1879 2371 2669 1000
Size: 879 492 298 2155
Fragments arranged by size:
2155 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3528 3528
Size: 3824
BsaAI yAC'GTr
Cuts at: 1177 1177
Size: 3824
BsaBI GATnn'nnATC
Cuts at: 6 1338 3375 6
Size: 1332 2037 455
Fragments arranged by size:
2037 1332 455
BsaHI Gr'CG_yC
Cuts at: 1255 1503 2205 3701 1255
Size: 248 702 1496 1378
Fragments arranged by size:
1496 1378 702 248
BsaWI w'CCGG_w
Cuts at: 712 1013 1534 2212 2486 2633 3044 712
Size: 301 521 678 274 147 411 1492
Fragments arranged by size:
1492 678 521 411 301 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 362 362
Size: 3824
BseYI C'CCAG_C
Cuts at: 1249 2007 2584 3080 1249
Size: 758 577 496 1993
Fragments arranged by size:
1993 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1366 1412 2620 104
Size: 1262 46 1208 1308
Fragments arranged by size:
1308 1262 1208 46
BsiHKAI G_wGCw'C
Cuts at: 368 838 1616 1806 2598 368
Size: 470 778 190 792 1594
Fragments arranged by size:
1594 792 778 470 190
BsmI GAATG_Cn'
Cuts at: 660 660
Size: 3824
Bsp1286I G_dGCh'C
Cuts at: 354 368 838 1449 1542 1616 1806 1868 2598
Size: 14 470 611 93 74 190 62 730
Cuts at: 2598 354
Size: 1580
Fragments arranged by size:
1580 730 611 470 190 93 74 62
14
BspEI T'CCGG_A
Cuts at: 3044 3044
Size: 3824
BspHI T'CATG_A
Cuts at: 3000 3000
Size: 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 295 874 1088 1390 1771 295
Size: 579 214 302 381 2348
Fragments arranged by size:
2348 579 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2116 2170 3654 3819 50
Size: 2066 54 1484 165 55
Fragments arranged by size:
2066 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1736 1736
Size: 3824
BsrFI r'CCGG_y
Cuts at: 1822 2003 2212 1822
Size: 181 209 3434
Fragments arranged by size:
3434 209 181
BssSI C'ACGA_G
Cuts at: 367 2095 2453 367
Size: 1728 358 1738
Fragments arranged by size:
1738 1728 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1201 1201
Size: 3824
BstBI TT'CG_AA
Cuts at: 2185 2185
Size: 3824
BstXI CCAn_nnnn'nTGG
Cuts at: 141 141
Size: 3824
BstYI r'GATC_y
Cuts at: 1 570 645 849 1316 1339 1674 1920 2932
Size: 569 75 204 467 23 335 246 1012
Cuts at: 2932 3018 3030 3376 1
Size: 86 12 346 449
Fragments arranged by size:
1012 569 467 449 346 335 246 204
86 75 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1774 1955 2118 1774
Size: 181 163 3480
Fragments arranged by size:
3480 181 163
BtsI GCAGTG_nn'
Cuts at: 299 1067 299
Size: 768 3056
DraI TTT'AAA
Cuts at: 823 823
Size: 3824
DrdI GACnn_nn'nnGTC
Cuts at: 1530 2388 1530
Size: 858 2966
EaeI y'GGCC_r
Cuts at: 1409 1583 1974 2001 3346 1409
Size: 174 391 27 1345 1887
Fragments arranged by size:
1887 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1409 1409
Size: 3824
EarI CTCTTCn'nnn_
Cuts at: 1847 2057 1847
Size: 210 3614
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2032 2342 2488 3634 2032
Size: 310 146 1146 2222
Fragments arranged by size:
2222 1146 310 146
EcoICRI GAG'CTC
Cuts at: 836 836
Size: 3824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 1649 2081 2828 3096 601
Size: 1048 432 747 268 1329
Fragments arranged by size:
1329 1048 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 963 963
Size: 3824
EcoRI G'AATT_C
Cuts at: 720 828 720
Size: 108 3716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1843 1843
Size: 3824
FspI TGC'GCA
Cuts at: 1605 1605
Size: 3824
HaeII r_GCGC'y
Cuts at: 1258 1506 2528 3507 1258
Size: 248 1022 979 1575
Fragments arranged by size:
1575 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 376 408 734 766 1666 1696 1698 1728 376
Size: 32 326 32 900 30 2 30 2472
Fragments arranged by size:
2472 900 326 32 32 30 30 2
HincII GTy'rAC
Cuts at: 863 863
Size: 3824
KasI G'GCGC_C
Cuts at: 1254 1502 1254
Size: 248 3576
KpnI G_GTAC'C
Cuts at: 731 844 731
Size: 113 3711
MluI A'CGCG_T
Cuts at: 3257 3257
Size: 3824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 147 394 2495 2679 92
Size: 55 247 2101 184 1237
Fragments arranged by size:
2101 1237 247 184 55
MscI TGG'CCA
Cuts at: 1585 1585
Size: 3824
MslI CAynn'nnrTG
Cuts at: 139 1813 1940 139
Size: 1674 127 2023
Fragments arranged by size:
2023 1674 127
MspA1I CmG'CkG
Cuts at: 806 935 1249 1609 2622 2867 806
Size: 129 314 360 1013 245 1763
Fragments arranged by size:
1763 1013 360 314 245 129
NaeI GCC'GGC
Cuts at: 2005 2005
Size: 3824
NarI GG'CG_CC
Cuts at: 1255 1503 1255
Size: 248 3576
NgoMIV G'CCGG_C
Cuts at: 2003 2003
Size: 3824
NspI r_CATG'y
Cuts at: 877 1377 2284 877
Size: 500 907 2417
Fragments arranged by size:
2417 907 500
PciI A'CATG_T
Cuts at: 2280 2280
Size: 3824
PmeI GTTT'AAAC
Cuts at: 823 823
Size: 3824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 3792
PstI C_TGCA'G
Cuts at: 306 871 1556 306
Size: 565 685 2574
Fragments arranged by size:
2574 685 565
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3824
PvuII CAG'CTG
Cuts at: 806 1249 1609 806
Size: 443 360 3021
Fragments arranged by size:
3021 443 360
RsrII CG'GwC_CG
Cuts at: 2019 2019
Size: 3824
SacI G_AGCT'C
Cuts at: 838 838
Size: 3824
SalI G'TCGA_C
Cuts at: 861 861
Size: 3824
SapI GCTCTTCn'nnn_
Cuts at: 1847 2057 1847
Size: 210 3614
SbfI CC_TGCA'GG
Cuts at: 871 871
Size: 3824
ScaI AGT'ACT
Cuts at: 3341 3341
Size: 3824
SfcI C'TryA_G
Cuts at: 33 302 549 807 867 1552 2545 2736 33
Size: 269 247 258 60 685 993 191 1121
Fragments arranged by size:
1121 993 685 269 258 247 191 60
SfoI GGC'GCC
Cuts at: 1256 1504 1256
Size: 248 3576
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3824
SmaI CCC'GGG
Cuts at: 846 846
Size: 3824
SmlI C'TyrA_G
Cuts at: 979 1894 2386 2648 979
Size: 915 492 262 2155
Fragments arranged by size:
2155 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3824
SphI G_CATG'C
Cuts at: 877 1377 877
Size: 500 3324
StuI AGG'CCT
Cuts at: 337 337
Size: 3824
StyI C'CwwG_G
Cuts at: 958 1312 958
Size: 354 3470
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1407 1540 1999 2208 1407
Size: 133 459 209 3023
Fragments arranged by size:
3023 459 209 133
TatI w'GTAC_w
Cuts at: 456 3339 456
Size: 2883 941
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 3792
Tth111I GACn'n_nGTC
Cuts at: 1621 1621
Size: 3824
XbaI T'CTAG_A
Cuts at: 855 855
Size: 3824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 1319 141
Size: 1178 2646
XmaI C'CCGG_G
Cuts at: 844 844
Size: 3824
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BpmI BsaXI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI