Restriction map for
pF1KB4223
(Linear) (Six-base) MAPSORT of: KIBB4223.seq Check: 9667 from: 1 to: 1248
KIBB4223.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 0 1043 1248
Size: 1043 205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 841 1248
Size: 811 30 407
Fragments arranged by size:
811 407 30
AflIII A'CryG_T
Cuts at: 0 409 608 1248
Size: 409 199 640
Fragments arranged by size:
640 409 199
AleI CACnn'nnGTG
Cuts at: 0 28 1248
Size: 28 1220
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 790 822 1248
Size: 790 32 426
Fragments arranged by size:
790 426 32
AlwNI CAG_nnn'CTG
Cuts at: 0 549 747 1248
Size: 549 198 501
Fragments arranged by size:
549 501 198
ApoI r'AATT_y
Cuts at: 0 354 1021 1248
Size: 354 667 227
Fragments arranged by size:
667 354 227
BanI G'GyrC_C
Cuts at: 0 39 1248
Size: 39 1209
BbeI G_GCGC'C
Cuts at: 0 43 1248
Size: 43 1205
BbvCI CC'TCA_GC
Cuts at: 0 127 991 1248
Size: 127 864 257
Fragments arranged by size:
864 257 127
BclI T'GATC_A
Cuts at: 0 339 1248
Size: 339 909
BglII A'GATC_T
Cuts at: 0 1181 1248
Size: 1181 67
BlpI GC'TnA_GC
Cuts at: 0 46 169 1248
Size: 46 123 1079
Fragments arranged by size:
1079 123 46
BmgBI CAC'GTC
Cuts at: 0 468 861 1248
Size: 468 393 387
Fragments arranged by size:
468 393 387
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1224 1248
Size: 1224 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 1248
Size: 802 446
Bpu10I CC'TnA_GC
Cuts at: 0 127 991 1199 1248
Size: 127 864 208 49
Fragments arranged by size:
864 208 127 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 1248
Size: 634 614
BsaHI Gr'CG_yC
Cuts at: 0 40 1040 1248
Size: 40 1000 208
Fragments arranged by size:
1000 208 40
BsaWI w'CCGG_w
Cuts at: 0 969 1248
Size: 969 279
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 775 895 910 928 979 1248
Size: 775 120 15 18 51 269
Fragments arranged by size:
775 269 120 51 18 15
BseYI C'CCAG_C
Cuts at: 0 268 1248
Size: 268 980
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1124 1248
Size: 1124 124
BsiEI CG_ry'CG
Cuts at: 0 432 1248
Size: 432 816
BspEI T'CCGG_A
Cuts at: 0 969 1248
Size: 969 279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 272 1248
Size: 272 976
BsrBI CCG'CTC
Cuts at: 0 45 900 1248
Size: 45 855 348
Fragments arranged by size:
855 348 45
BsrDI GCAATG_nn'
Cuts at: 0 180 1248
Size: 180 1068
BsrFI r'CCGG_y
Cuts at: 0 431 1248
Size: 431 817
BstAPI GCAn_nnn'nTGC
Cuts at: 0 747 1147 1248
Size: 747 400 101
Fragments arranged by size:
747 400 101
BstEII G'GTnAC_C
Cuts at: 0 597 1248
Size: 597 651
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 1248
Size: 30 1218
BstYI r'GATC_y
Cuts at: 0 1181 1248
Size: 1181 67
BtgI C'CryG_G
Cuts at: 0 23 299 569 1248
Size: 23 276 270 679
Fragments arranged by size:
679 276 270 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 619 1248
Size: 619 629
DraIII CAC_nnn'GTG
Cuts at: 0 861 1248
Size: 861 387
EaeI y'GGCC_r
Cuts at: 0 1094 1248
Size: 1094 154
EarI CTCTTCn'nnn_
Cuts at: 0 253 1248
Size: 253 995
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 487 1248
Size: 487 761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 811 841 1248
Size: 802 9 30 407
Fragments arranged by size:
802 407 30 9
EcoRI G'AATT_C
Cuts at: 0 354 1021 1248
Size: 354 667 227
Fragments arranged by size:
667 354 227
EcoRV GAT'ATC
Cuts at: 0 1107 1248
Size: 1107 141
HaeII r_GCGC'y
Cuts at: 0 43 53 374 438 1248
Size: 43 10 321 64 810
Fragments arranged by size:
810 321 64 43 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1216 1248
Size: 1216 32
HincII GTy'rAC
Cuts at: 0 1038 1248
Size: 1038 210
KasI G'GCGC_C
Cuts at: 0 39 1248
Size: 39 1209
MluI A'CGCG_T
Cuts at: 0 409 1248
Size: 409 839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 1248
Size: 32 1216
MscI TGG'CCA
Cuts at: 0 1096 1248
Size: 1096 152
MslI CAynn'nnrTG
Cuts at: 0 28 196 1248
Size: 28 168 1052
Fragments arranged by size:
1052 168 28
MspA1I CmG'CkG
Cuts at: 0 819 1248
Size: 819 429
NarI GG'CG_CC
Cuts at: 0 40 1248
Size: 40 1208
NcoI C'CATG_G
Cuts at: 0 299 1248
Size: 299 949
NspI r_CATG'y
Cuts at: 0 558 754 1147 1248
Size: 558 196 393 101
Fragments arranged by size:
558 393 196 101
PflMI CCAn_nnn'nTGG
Cuts at: 0 861 1248
Size: 861 387
PfoI T'CCnGG_A
Cuts at: 0 779 1248
Size: 779 469
PvuII CAG'CTG
Cuts at: 0 819 1248
Size: 819 429
ScaI AGT'ACT
Cuts at: 0 1207 1248
Size: 1207 41
SfcI C'TryA_G
Cuts at: 0 219 834 1248
Size: 219 615 414
Fragments arranged by size:
615 414 219
SfoI GGC'GCC
Cuts at: 0 41 1248
Size: 41 1207
SmlI C'TyrA_G
Cuts at: 0 649 1248
Size: 649 599
StyI C'CwwG_G
Cuts at: 0 299 695 1248
Size: 299 396 553
Fragments arranged by size:
553 396 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 925 1248
Size: 925 323
TatI w'GTAC_w
Cuts at: 0 1205 1248
Size: 1205 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 901 933 1248
Size: 901 32 315
Fragments arranged by size:
901 315 32
Tth111I GACn'n_nGTC
Cuts at: 0 453 1248
Size: 453 795
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 400 465 1248
Size: 400 65 783
Fragments arranged by size:
783 400 65
XmnI GAAnn'nnTTC
Cuts at: 0 79 103 610 1248
Size: 79 24 507 638
Fragments arranged by size:
638 507 79 24
ZraI GAC'GTC
Cuts at: 0 1041 1248
Size: 1041 207
Enzymes that do cut:
AatII AcuI AflIII AleI AloI AlwNI ApoI BanI
BbeI BbvCI BclI BglII BlpI BmgBI BplI BpmI
Bpu10I BpuEI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI
BstYI BtgI BtgZI DraIII EaeI EarI EciI Eco57MI
EcoRI EcoRV HaeII Hin4I HincII KasI MluI MmeI
MscI MslI MspA1I NarI NcoI NspI PflMI PfoI
PvuII ScaI SfcI SfoI SmlI StyI TaqII TatI
TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbsI BcgI BciVI BfrBI BglI
Bme1580I BmrI BmtI BsaI BsaAI BsaBI BsaXI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspHI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI
EagI EcoICRI EcoNI EcoO109I FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XhoI XmaI