Restriction map for pF1KB4099
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AflIII      AgeI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmI        BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BssHII      Bst1107I    BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraIII      
DrdI        EaeI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        Tth111I     XbaI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AclI          214  AACGTT               AA^CGTT
AcsI          192  AAATTT               [GA]^AATT[CT]
AhdI           91  GACTCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         845  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          192  AAATTT               [GA]^AATT[CT]
AspEI          91  GACTCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         478  GTGCTC               G[AT]GC[AT]^C
AvaI         1265  CTCGAG               C^[CT]CG[GA]G
BanI          415  GGTGCC               G^G[CT][GA]CC
BcnI         1239  CCGGG                CC^[GC]GG
BmyI          478  GTGCTC               G[AGT]GC[ACT]^C
BsaI          930  GGTCTC               GGTCTC(1/5)
BsiHKAI       478  GTGCTC               G[AT]GC[AT]^C
BsmBI        1020  CGTCTC               CGTCTC(1/5)
BsmFI         515  GTCCC                GGGAC(10/14)
BsoBI        1265  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      478  GTGCTC               G[AGT]GC[ACT]^C
BsrFI           5  ACCGGC               [GA]^CCGG[CT]
BssSI        1094  CTCGTG               CACGAG(-5/-1)
BstAPI        844  GCAGCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         462  TTCGAA               TT^CGAA
CfoI         1047  GCGC                 GCG^C
Cfr10I          5  ACCGGC               [GA]^CCGG[CT]
DraI          196  TTTAAA               TTT^AAA
DraII         201  AGGTCCT              [GA]G^G[ACGT]CC[CT]
Eam1105I       91  GACTCCAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      201  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRV        1260  GATATC               GAT^ATC
HhaI         1047  GCGC                 GCG^C
HinP1I       1047  GCGC                 G^CGC
NciI         1239  CCGGG                CC^[GC]GG
NspV          462  TTCGAA               TT^CGAA
PaeR7I       1265  CTCGAG               C^TCGAG
PflMI        1201  CCAAAAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         201  AGGTCCT              [GA]G^G[AT]CC[CT]
Psp1406I      214  AACGTT               AA^CGTT
SfcI          483  CTACAG               C^T[GA][CT]AG
SfuI          462  TTCGAA               TT^CGAA
SmlI         1265  CTCGAG               C^T[CT][GA]AG
Van91I       1201  CCAAAAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1265  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

BbsI         1058  GAAGAC               GAAGAC(2/6)
             1084  GAAGAC              
BfaI           13  CTAG                 C^TAG
              370  CTAG                
BpuAI        1058  GAAGAC               GAAGAC(2/6)
             1084  GAAGAC              
BsaBI         780  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              948  GATTGACATC          
BspMI         724  ACCTGC               ACCTGC(4/8)
             1176  ACCTGC              
Cac8I         318  GCTGGC               GC[ACGT]^[ACGT]GC
              794  GCCTGC              
DdeI           48  CTTAG                C^T[ACGT]AG
              292  CTGAG               
DsaI          266  CCATGG               C^C[GA][CT]GG
              518  CCATGG              
EarI          673  GAAGAG               CTCTTC(1/4)
             1065  GAAGAG              
EcoT14I       266  CCATGG               C^C[AT][AT]GG
              518  CCATGG              
HapII           6  CCGG                 C^CGG
             1239  CCGG                
HpaII           6  CCGG                 C^CGG
             1239  CCGG                
Ksp632I       673  GAAGAG               CTCTTC(1/4)
             1065  GAAGAG              
MaeI           13  CTAG                 C^TAG
              370  CTAG                
MaeII         215  ACGT                 A^CGT
             1088  ACGT                
MamI          780  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              948  GATTGACATC          
MslI          954  CATCTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1336  CATGAAGATG          
MspI            6  CCGG                 C^CGG
             1239  CCGG                
NcoI          266  CCATGG               C^CATGG
              518  CCATGG              
PvuII         565  CAGCTG               CAG^CTG
             1160  CAGCTG              
SspI          226  AATATT               AAT^ATT
              283  AATATT              
StyI          266  CCATGG               C^C[AT][AT]GG
              518  CCATGG              
TaqI          463  TCGA                 T^CGA
             1266  TCGA                
TfiI          182  GAATC                G^A[AT]TC
              310  GATTC               
TthHB8I       463  TCGA                 T^CGA
             1266  TCGA                
XcmI          854  CCAGGGTCGCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1143  CCAAGTGAAAGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BbvI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1407I    BsrGI       BsrI        BssKI       
BstF5I      BstNI       Cfr13I      DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeIII      MboI        MboII       MnlI        
MseI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SspBI       Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       
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