Restriction map for pF1KB4080
 (Linear) (Six-base) MAPSORT of: KIBB4080.seq  Check: 4588  from: 1  to: 1014



KIBB4080.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AccI GT'mk_AC

Cuts at:      0     675    1014 
   Size:        675     339
 
Acc65I G'GTAC_C

Cuts at:      0     613    1014 
   Size:        613     401
 
AfeI AGC'GCT

Cuts at:      0     304    1014 
   Size:        304     710
 
AflIII A'CryG_T

Cuts at:      0      83     137    1014 
   Size:         83      54     877

  Fragments arranged by size: 

                 877      83      54
 
ApoI r'AATT_y

Cuts at:      0     241     503     512     625     776     915    1014 
   Size:        241     262       9     113     151     139      99

  Fragments arranged by size: 

                 262     241     151     139     113      99       9
 
AseI AT'TA_AT

Cuts at:      0     590    1014 
   Size:        590     424
 
AvaI C'yCGr_G

Cuts at:      0     839    1014 
   Size:        839     175
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     389     422    1014 
   Size:        389      33     592

  Fragments arranged by size: 

                 592     389      33
 
BanI G'GyrC_C

Cuts at:      0     613    1014 
   Size:        613     401
 
BanII G_rGCy'C

Cuts at:      0     321    1014 
   Size:        321     693
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     481    1014 
   Size:        481     533
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     144    1014 
   Size:        144     870
 
BglII A'GATC_T

Cuts at:      0     956    1014 
   Size:        956      58
 
BlpI GC'TnA_GC

Cuts at:      0     557    1014 
   Size:        557     457
 
BmgBI CAC'GTC

Cuts at:      0      86    1014 
   Size:         86     928
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      90     393    1014 
   Size:         90     303     621

  Fragments arranged by size: 

                 621     303      90
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     386     547    1014 
   Size:        386     161     467

  Fragments arranged by size: 

                 467     386     161
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     856     886    1014 
   Size:        856      30     128

  Fragments arranged by size: 

                 856     128      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412    1014 
   Size:        412     602
 
BsmI GAATG_Cn'

Cuts at:      0     644    1014 
   Size:        644     370
 
Bsp1286I G_dGCh'C

Cuts at:      0     321    1014 
   Size:        321     693
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     383     781    1014 
   Size:        383     398     233

  Fragments arranged by size: 

                 398     383     233
 
BstYI r'GATC_y

Cuts at:      0     956    1014 
   Size:        956      58
 
BstZ17I GTA'TAC

Cuts at:      0     676    1014 
   Size:        676     338
 
BtsI GCAGTG_nn'

Cuts at:      0     183     627    1014 
   Size:        183     444     387

  Fragments arranged by size: 

                 444     387     183
 
EcoRI G'AATT_C

Cuts at:      0     503     512    1014 
   Size:        503       9     502

  Fragments arranged by size: 

                 503     502       9
 
EcoRV GAT'ATC

Cuts at:      0     186    1014 
   Size:        186     828
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     276     321    1014 
   Size:        276      45     693

  Fragments arranged by size: 

                 693     276      45
 
HaeII r_GCGC'y

Cuts at:      0     306    1014 
   Size:        306     708
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     468     500    1014 
   Size:        468      32     514

  Fragments arranged by size: 

                 514     468      32
 
HindIII A'AGCT_T

Cuts at:      0     195    1014 
   Size:        195     819
 
KpnI G_GTAC'C

Cuts at:      0     617    1014 
   Size:        617     397
 
MslI CAynn'nnrTG

Cuts at:      0     370    1014 
   Size:        370     644
 
MspA1I CmG'CkG

Cuts at:      0     801     929    1014 
   Size:        801     128      85

  Fragments arranged by size: 

                 801     128      85
 
NspI r_CATG'y

Cuts at:      0     141     885    1014 
   Size:        141     744     129

  Fragments arranged by size: 

                 744     141     129
 
PciI A'CATG_T

Cuts at:      0     137    1014 
   Size:        137     877
 
PspXI vC'TCGA_Gb

Cuts at:      0     839    1014 
   Size:        839     175
 
PvuII CAG'CTG

Cuts at:      0     929    1014 
   Size:        929      85
 
ScaI AGT'ACT

Cuts at:      0     997    1014 
   Size:        997      17
 
SmlI C'TyrA_G

Cuts at:      0     401     562     839    1014 
   Size:        401     161     277     175

  Fragments arranged by size: 

                 401     277     175     161
 
TatI w'GTAC_w

Cuts at:      0     995    1014 
   Size:        995      19
 
XhoI C'TCGA_G

Cuts at:      0     839    1014 
   Size:        839     175
 
XmnI GAAnn'nnTTC

Cuts at:      0     769    1014 
   Size:        769     245
 

 Enzymes that do cut:

     AccI   Acc65I     AfeI   AflIII     ApoI     AseI     AvaI     BaeI
     BanI    BanII     BbsI    BciVI    BglII     BlpI    BmgBI     BmrI
    BpuEI    BsaXI     BsgI     BsmI Bsp1286I    BstXI    BstYI  BstZ17I
     BtsI    EcoRI    EcoRV     FalI    HaeII    Hin4I  HindIII     KpnI
     MslI   MspA1I     NspI     PciI    PspXI    PvuII     ScaI     SmlI
     TatI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI    AvrII
    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI Bme1580I
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FseI     FspI    FspAI   HincII     HpaI
     KasI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XmaI
     ZraI
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