Restriction map for pF1KB4044
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
AocI        Aor51HI     ApaI        AscI        Asp718I     
AspEI       AspI        AvaI        AviII       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmAI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     XhoI        XmaCI       
XmaI        


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# ONE CUT ENZYMES (   44 enzymes) #
###################################

Alw44I       1440  GTGCAC               G^TGCAC
ApaLI        1440  GTGCAC               G^TGCAC
AseI           91  ATTAAT               AT^TAAT
AsnI           91  ATTAAT               AT^TAAT
Asp700I      1165  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII        1278  GGACC                G^G[AT]CC
AvrII        1465  CCTAGG               C^CTAGG
BalI          483  TGGCCA               TGG^CCA
BamHI         540  GGATCC               G^GATCC
BcnI         1067  CCGGG                CC^[GC]GG
BglII         815  AGATCT               A^GATCT
BlnI         1465  CCTAGG               C^CTAGG
BlpI          971  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      971  GCTTAGC              GC^T[ACGT]AGC
BseRI         740  GAGGAG               GAGGAG(10/8)
BsgI          586  CTGCAC               GTGCAG(16/14)
BsmI         1432  GAATGC               GAATGC(1/-1)
BsrBI        1084  GAGCGG               CCGCTC(-3/-3)
BstAPI       1479  GCAGCATGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         605  TTCGAA               TT^CGAA
CelII         971  GCTTAGC              GC^T[ACGT]AGC
DsaI          794  CCATGG               C^C[GA][CT]GG
HapII        1067  CCGG                 C^CGG
HincII       1015  GTTGAC               GT[CT]^[GA]AC
HindII       1015  GTTGAC               GT[CT]^[GA]AC
HpaII        1067  CCGG                 C^CGG
MluNI         483  TGGCCA               TGG^CCA
MscI          483  TGGCCA               TGG^CCA
MspI         1067  CCGG                 C^CGG
MwoI         1479  GCAGCATGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI         1067  CCGGG                CC^[GC]GG
NcoI          794  CCATGG               C^CATGG
NspV          605  TTCGAA               TT^CGAA
PflMI         528  CCATGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI          91  ATTAAT               AT^TAAT
SacI          743  GAGCTC               GAGCT^C
SfuI          605  TTCGAA               TT^CGAA
SmlI         1138  CTCAAG               C^T[CT][GA]AG
StuI          164  AGGCCT               AGG^CCT
TaqI          606  TCGA                 T^CGA
TthHB8I       606  TCGA                 T^CGA
Van91I        528  CCATGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1026  TCTAGA               T^CTAGA
XmnI         1165  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwNI         580  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1511  CAGGGCCTG           
BpmI           37  CTGGAG               CTGGAG(16/14)
              535  CTGGAG              
BsmFI         631  GGGAC                GGGAC(10/14)
             1277  GGGAC               
BspLU11I      282  ACATGT               A^CATGT
             1372  ACATGT              
BspMI         584  ACCTGC               ACCTGC(4/8)
             1327  ACCTGC              
Cac8I        1270  GCCCGC               GC[ACGT]^[ACGT]GC
             1313  GCCAGC              
DdeI          887  CTCAG                C^T[ACGT]AG
              972  CTTAG               
DraII        1404  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1512  AGGGCCT             
EaeI          483  TGGCCA               [CT]^GGCC[GA]
             1453  CGGCCA              
EarI         1044  GAAGAG               CTCTTC(1/4)
             1165  GAAGAG              
EcoO109I     1404  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1512  AGGGCCT             
FauI         1271  CCCGC                CCCGC(4/6)
             1086  GCGGG               
Ksp632I      1044  GAAGAG               CTCTTC(1/4)
             1165  GAAGAG              
MaeII         428  ACGT                 A^CGT
             1013  ACGT                
MspA1I       1077  CCGCTG               C[AC]G^C[GT]G
             1272  CCGCTG              
PleI           84  GAGTC                GAGTC(4/5)
              369  GAGTC               
PstI          380  CTGCAG               CTGCA^G
              614  CTGCAG              
SfcI          380  CTGCAG               C^T[GA][CT]AG
              614  CTGCAG              
Tsp45I        349  GTGAC                ^GT[GC]AC
             1369  GTCAC               
XcmI          659  CCAGACTTTATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1134  CCAGCTCAAGGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AspHI       BanII       BbvI        
BfaI        BmyI        BsaJI       BsiHKAI     BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cfr13I      DpnII       DraI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MvaI        NdeII       NlaIII      NlaIV       
NspI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XhoII       
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