Restriction map for pF1KB4044
 (Linear) (Six-base) MAPSORT of: KIBB4044.seq  Check: 9414  from: 1  to: 1548



KIBB4044.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     593    1548 
   Size:        593     955
 
AflIII A'CryG_T

Cuts at:      0     282     428    1372    1548 
   Size:        282     146     944     176

  Fragments arranged by size: 

                 944     282     176     146
 
AlwNI CAG_nnn'CTG

Cuts at:      0     585    1516    1548 
   Size:        585     931      32

  Fragments arranged by size: 

                 931     585      32
 
ApaLI G'TGCA_C

Cuts at:      0    1440    1548 
   Size:       1440     108
 
ApoI r'AATT_y

Cuts at:      0     308     856     991    1548 
   Size:        308     548     135     557

  Fragments arranged by size: 

                 557     548     308     135
 
AseI AT'TA_AT

Cuts at:      0      92    1548 
   Size:         92    1456
 
AvrII C'CTAG_G

Cuts at:      0    1465    1548 
   Size:       1465      83
 
BamHI G'GATC_C

Cuts at:      0     540    1548 
   Size:        540    1008
 
BanII G_rGCy'C

Cuts at:      0     747    1272    1504    1548 
   Size:        747     525     232      44

  Fragments arranged by size: 

                 747     525     232      44
 
BglII A'GATC_T

Cuts at:      0     815    1548 
   Size:        815     733
 
BlpI GC'TnA_GC

Cuts at:      0     972    1548 
   Size:        972     576
 
Bme1580I G_kGCm'C

Cuts at:      0     794    1444    1548 
   Size:        794     650     104

  Fragments arranged by size: 

                 794     650     104
 
BmgBI CAC'GTC

Cuts at:      0     429    1548 
   Size:        429    1119
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1299    1548 
   Size:       1299     249
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     556    1548 
   Size:         58     498     992

  Fragments arranged by size: 

                 992     498      58
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1123    1548 
   Size:       1123     425
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     755    1548 
   Size:        755     793
 
BseYI C'CCAG_C

Cuts at:      0      97    1274    1548 
   Size:         97    1177     274

  Fragments arranged by size: 

                1177     274      97
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571    1548 
   Size:        571     977
 
BsiHKAI G_wGCw'C

Cuts at:      0     361     512     747    1444    1548 
   Size:        361     151     235     697     104

  Fragments arranged by size: 

                 697     361     235     151     104
 
BsmI GAATG_Cn'

Cuts at:      0    1438    1548 
   Size:       1438     110
 
Bsp1286I G_dGCh'C

Cuts at:      0     361     512     747     794    1272    1444    1504    1548 
   Size:        361     151     235      47     478     172      60      44

  Fragments arranged by size: 

                 478     361     235     172     151      60      47      44
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     593    1336    1548 
   Size:        593     743     212

  Fragments arranged by size: 

                 743     593     212
 
BsrBI CCG'CTC

Cuts at:      0    1086    1548 
   Size:       1086     462
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1485    1548 
   Size:       1485      63
 
BstBI TT'CG_AA

Cuts at:      0     606    1548 
   Size:        606     942
 
BstYI r'GATC_y

Cuts at:      0     540     551     815    1548 
   Size:        540      11     264     733

  Fragments arranged by size: 

                 733     540     264      11
 
BtgI C'CryG_G

Cuts at:      0     794    1548 
   Size:        794     754
 
BtsI GCAGTG_nn'

Cuts at:      0     498    1548 
   Size:        498    1050
 
DraI TTT'AAA

Cuts at:      0      74     335     401    1425    1548 
   Size:         74     261      66    1024     123

  Fragments arranged by size: 

                1024     261     123      74      66
 
EaeI y'GGCC_r

Cuts at:      0     483    1453    1548 
   Size:        483     970      95

  Fragments arranged by size: 

                 970     483      95
 
EarI CTCTTCn'nnn_

Cuts at:      0    1039    1160    1548 
   Size:       1039     121     388

  Fragments arranged by size: 

                1039     388     121
 
EcoICRI GAG'CTC

Cuts at:      0     745    1548 
   Size:        745     803
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     556    1548 
   Size:         58     498     992

  Fragments arranged by size: 

                 992     498      58
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1405    1513    1548 
   Size:       1405     108      35

  Fragments arranged by size: 

                1405     108      35
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     427     697    1548 
   Size:        427     270     851

  Fragments arranged by size: 

                 851     427     270
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1231    1263    1548 
   Size:       1231      32     285

  Fragments arranged by size: 

                1231     285      32
 
HincII GTy'rAC

Cuts at:      0    1017    1548 
   Size:       1017     531
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     223    1548 
   Size:        223    1325
 
MscI TGG'CCA

Cuts at:      0     485    1548 
   Size:        485    1063
 
MslI CAynn'nnrTG

Cuts at:      0     281     302     422    1548 
   Size:        281      21     120    1126

  Fragments arranged by size: 

                1126     281     120      21
 
MspA1I CmG'CkG

Cuts at:      0    1079    1274    1548 
   Size:       1079     195     274

  Fragments arranged by size: 

                1079     274     195
 
NcoI C'CATG_G

Cuts at:      0     794    1548 
   Size:        794     754
 
NspI r_CATG'y

Cuts at:      0     286     301    1376    1486    1548 
   Size:        286      15    1075     110      62

  Fragments arranged by size: 

                1075     286     110      62      15
 
PciI A'CATG_T

Cuts at:      0     282    1372    1548 
   Size:        282    1090     176

  Fragments arranged by size: 

                1090     282     176
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     534    1548 
   Size:        534    1014
 
PstI C_TGCA'G

Cuts at:      0     384     618    1548 
   Size:        384     234     930

  Fragments arranged by size: 

                 930     384     234
 
SacI G_AGCT'C

Cuts at:      0     747    1548 
   Size:        747     801
 
SfcI C'TryA_G

Cuts at:      0     380     614    1548 
   Size:        380     234     934

  Fragments arranged by size: 

                 934     380     234
 
SmlI C'TyrA_G

Cuts at:      0    1138    1548 
   Size:       1138     410
 
StuI AGG'CCT

Cuts at:      0     166    1548 
   Size:        166    1382
 
StyI C'CwwG_G

Cuts at:      0      24     794     963    1109    1465    1548 
   Size:         24     770     169     146     356      83

  Fragments arranged by size: 

                 770     356     169     146      83      24
 
TatI w'GTAC_w

Cuts at:      0     130    1058    1548 
   Size:        130     928     490

  Fragments arranged by size: 

                 928     490     130
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     624     656     694     726    1423    1455    1548 
   Size:        624      32      38      32     697      32      93

  Fragments arranged by size: 

                 697     624      93      38      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1026    1548 
   Size:       1026     522
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     666    1141    1548 
   Size:        666     475     407

  Fragments arranged by size: 

                 666     475     407
 
XmnI GAAnn'nnTTC

Cuts at:      0    1169    1548 
   Size:       1169     379
 

 Enzymes that do cut:

     AarI   AflIII    AlwNI    ApaLI     ApoI     AseI    AvrII    BamHI
    BanII    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI    BpuEI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI
   BstAPI    BstBI    BstYI     BtgI     BtsI     DraI     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I     FalI    Hin4I   HincII     MmeI     MscI
     MslI   MspA1I     NcoI     NspI     PciI    PflMI     PstI     SacI
     SfcI     SmlI     StuI     StyI     TatI     TstI     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     AscI    AsiSI     AvaI
     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EciI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII  Tth111I
     XhoI     XmaI     ZraI
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