Restriction map for pF1KB3964
 (Linear) (Six-base) MAPSORT of: KIBB3964.seq  Check: 8226  from: 1  to: 567



KIBB3964.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      22     567 
   Size:         22     545
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     478     511     567 
   Size:        478      33      56

  Fragments arranged by size: 

                 478      56      33
 
BbvCI CC'TCA_GC

Cuts at:      0     432     567 
   Size:        432     135
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     162     567 
   Size:        162     405
 
Bpu10I CC'TnA_GC

Cuts at:      0     432     567 
   Size:        432     135
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     197     567 
   Size:        197     370
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      22     567 
   Size:         22     545
 
BtsI GCAGTG_nn'

Cuts at:      0     525     567 
   Size:        525      42
 
EaeI y'GGCC_r

Cuts at:      0     398     567 
   Size:        398     169
 
EarI CTCTTCn'nnn_

Cuts at:      0     179     567 
   Size:        179     388
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     137     567 
   Size:          6     131     430

  Fragments arranged by size: 

                 430     131       6
 
MscI TGG'CCA

Cuts at:      0     400     567 
   Size:        400     167
 
MspA1I CmG'CkG

Cuts at:      0     175     567 
   Size:        175     392
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     396     567 
   Size:        396     171
 
PvuII CAG'CTG

Cuts at:      0     175     567 
   Size:        175     392
 
SfcI C'TryA_G

Cuts at:      0     285     567 
   Size:        285     282
 
TatI w'GTAC_w

Cuts at:      0       8     188     567 
   Size:          8     180     379

  Fragments arranged by size: 

                 379     180       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      44      76     567 
   Size:         44      32     491

  Fragments arranged by size: 

                 491      44      32
 

 Enzymes that do cut:

     AarI     BaeI    BbvCI    BciVI   Bpu10I     BsaI    BspMI     BtsI
     EaeI     EarI     MmeI     MscI   MspA1I    PflMI    PvuII     SfcI
     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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