Restriction map for pF1KB3919
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AfaI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BalI        BbeI        BbrPI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspEI       BspMI       BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CelII       Cfr10I      
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MfeI        MluNI       
MroI        MscI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfoI        SfuI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII         866  GACGTC               GACGT^C
AccII         117  CGCG                 CG^CG
AocI         1157  CCTCAGG              CC^T[ACGT]AGG
AspHI         211  GAGCAC               G[AT]GC[AT]^C
AspI          672  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          503  CCCGAG               C^[CT]CG[GA]G
BamHI         556  GGATCC               G^GATCC
BbsI          105  GAAGAC               GAAGAC(2/6)
BciVI         562  GTATCC               GTATCC(6/5)
BpuAI         105  GAAGAC               GAAGAC(2/6)
BsaI          548  GGTCTC               GGTCTC(1/5)
BsiHKAI       211  GAGCAC               G[AT]GC[AT]^C
BsoBI         503  CCCGAG               C^[CT]CG[GA]G
BspDI         685  ATCGAT               AT^CGAT
BspHI        1028  TCATGA               T^CATGA
BspLU11I     1003  ACATGT               A^CATGT
BssSI        1571  CTCGTG               CACGAG(-5/-1)
BstAPI       1336  GCACCAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         117  CGCG                 CG^CG
BstXI         612  CCAATTTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1157  CCTCAGG              CC^T[ACGT]AGG
ClaI          685  ATCGAT               AT^CGAT
EaeI          473  TGGCCG               [CT]^GGCC[GA]
Eco81I       1157  CCTCAGG              CC^T[ACGT]AGG
EcoRV         933  GATATC               GAT^ATC
EcoT14I      1115  CCTTGG               C^C[AT][AT]GG
EcoT22I       790  ATGCAT               ATGCA^T
HaeII         165  GGCGCT               [GA]GCGC^[CT]
MaeII         867  ACGT                 A^CGT
MluI          116  ACGCGT               A^CGCGT
MslI         1624  CACTTCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1328  CAGCGG               C[AC]G^C[GT]G
MvnI          117  CGCG                 CG^CG
NsiI          790  ATGCAT               ATGCA^T
NspI         1003  ACATGT               [GA]CATG^[CT]
PflFI         672  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI          90  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          356  CTGCAG               CTGCA^G
RcaI         1028  TCATGA               T^CATGA
SfcI          356  CTGCAG               C^T[GA][CT]AG
SfiI          147  GGCCCTCAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI          300  ACTAGT               A^CTAGT
StyI         1115  CCTTGG               C^C[AT][AT]GG
Tth111I       672  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcyI           70  GACGCC               G[GA]^CG[CT]C
              866  GACGTC              
AflIII        116  ACGCGT               A^C[GA][CT]GT
             1003  ACATGT              
ApaI          146  GGGCCC               GGGCC^C
             1162  GGGCCC              
BbvCI          24  CCTCAGC              CCTCAGC(-5/-2)
             1015  GCTGAGG             
BcgI          231  GCAAAGGATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1287  GCAGCTTTCTCG        
BglI          148  GCCCTCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              157  GCCATCGAGGC         
BsaBI         557  GATCCGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              729  GATTACTATC          
BsaHI          70  GACGCC               G[GA]^CG[CT]C
              866  GACGTC              
BsgI          187  CTGCAC               GTGCAG(16/14)
             1553  GTGCAG              
BsrDI         941  CATTGC               GCAATG(2/0)
              992  GCAATG              
CfoI          166  GCGC                 GCG^C
              594  GCGC                
EarI          823  GAAGAG               CTCTTC(1/4)
             1450  GAAGAG              
HgaI           70  GACGC                GACGC(5/10)
              954  GACGC               
HhaI          166  GCGC                 GCG^C
              594  GCGC                
Hin1I          70  GACGCC               G[GA]^CG[CT]C
              866  GACGTC              
HinP1I        166  GCGC                 G^CGC
              594  GCGC                
Ksp632I       823  GAAGAG               CTCTTC(1/4)
             1450  GAAGAG              
MamI          557  GATCCGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              729  GATTACTATC          
SfaNI        1143  GATGC                GCATC(5/9)
             1342  GATGC               
TfiI          237  GATTC                G^A[AT]TC
             1074  GAATC               
Tsp45I       1574  GTGAC                ^GT[GC]AC
             1622  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AvaII       BanI        BanII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       SmlI        
TaqI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     XhoII       
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