Restriction map for pF1KB3650
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AflIII      AgeI        Alw44I      AlwI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
AspI        AvaI        AviII       AvrII       BamHI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspEI       BspHI       
BspLU11I    BspMI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstYI       BstZ17I     CelII       CpoI        DpnII       
DraI        DrdI        EagI        EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KspI        MaeI        MamI        
MboI        MfeI        MflI        MluI        MroI        
MseI        MunI        NdeI        NdeII       NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacII       SalI        
SapI        Sau3AI      SbfI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       Tsp509I     Tth111I     XbaI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          811  GTCTAC               GT^[AC][GT]AC
AhdI           58  GACCAAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1418  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       474  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI          58  GACCAAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         124  GAGCTC               G[AT]GC[AT]^C
BbeI          382  GGCGCC               GGCGC^C
BbrPI        1197  CACGTG               CAC^GTG
BbvCI         439  CCTCAGC              CCTCAGC(-5/-2)
BglI           31  GCCCTGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1197  CACGTG               [CT]AC^GT[GA]
BsiHKAI       124  GAGCTC               G[AT]GC[AT]^C
BspDI         616  ATCGAT               AT^CGAT
BsrBI         805  GAGCGG               CCGCTC(-3/-3)
BsrFI        1181  GCCGGC               [GA]^CCGG[CT]
BstXI        1152  CCACAGCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I       1181  GCCGGC               [GA]^CCGG[CT]
ClaI          616  ATCGAT               AT^CGAT
DraIII       1018  CACGGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I       58  GACCAAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         660  CCTCTTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI          382  GGCGCC               G^GCGCC
MslI         1153  CACAGCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         1181  GCCGGC               GCC^GGC
NarI          382  GGCGCC               GG^CGCC
NcoI         1046  CCATGG               C^CATGG
NgoMIV       1181  GCCGGC               G^CCGGC
PflMI        1417  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1197  CACGTG               CAC^GTG
PmlI         1197  CACGTG               CAC^GTG
PvuII         933  CAGCTG               CAG^CTG
SacI          124  GAGCTC               GAGCT^C
SfoI          382  GGCGCC               GGC^GCC
TfiI          324  GAATC                G^A[AT]TC
Van91I       1417  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          786  CCATAGCTACAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          474  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Acc65I        572  GGTACC               G^GTACC
              982  GGTACC              
AccII         190  CGCG                 CG^CG
             1192  CGCG                
AcyI          382  GGCGCC               G[GA]^CG[CT]C
              484  GACGCC              
ApaI          282  GGGCCC               GGGCC^C
              609  GGGCCC              
Asp718I       572  GGTACC               G^GTACC
              982  GGTACC              
AvaII         705  GGACC                G^G[AT]CC
             1371  GGACC               
BalI         1094  TGGCCA               TGG^CCA
             1425  TGGCCA              
BbsI          651  GAAGAC               GAAGAC(2/6)
             1306  GTCTTC              
BpuAI         651  GAAGAC               GAAGAC(2/6)
             1306  GTCTTC              
BsaHI         382  GGCGCC               G[GA]^CG[CT]C
              484  GACGCC              
BsmFI         929  GGGAC                GGGAC(10/14)
             1010  GGGAC               
BstAPI         40  GCATGGGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1017  GCACGGTGTGC         
BstUI         190  CGCG                 CG^CG
             1192  CGCG                
DsaI         1046  CCATGG               C^C[GA][CT]GG
             1158  CCGTGG              
HaeII         382  GGCGCC               [GA]GCGC^[CT]
              683  GGCGCT              
HgaI          484  GACGC                GACGC(5/10)
             1039  GACGC               
Hin1I         382  GGCGCC               G[GA]^CG[CT]C
              484  GACGCC              
HphI          491  TCACC                GGTGA(8/7)
              629  TCACC               
KpnI          572  GGTACC               GGTAC^C
              982  GGTACC              
MaeIII        119  GTGAC                ^GT[ACGT]AC
              184  GTGAC               
MluNI        1094  TGGCCA               TGG^CCA
             1425  TGGCCA              
MscI         1094  TGGCCA               TGG^CCA
             1425  TGGCCA              
MvnI          190  CGCG                 CG^CG
             1192  CGCG                
SfcI          792  CTACAG               C^T[GA][CT]AG
             1257  CTACAG              
SmlI          408  CTCAAG               C^T[CT][GA]AG
              499  CTCAAG              
StuI          412  AGGCCT               AGG^CCT
              565  AGGCCT              
Tsp45I        119  GTGAC                ^GT[GC]AC
              184  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AfaI        AluI        AocI        BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaI        BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DraII       EaeI        EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        Ksp632I     
MaeII       MboII       MnlI        MspA1I      MspI        
MvaI        MwoI        NciI        NlaIII      NlaIV       
PleI        RsaI        Sau96I      ScaI        ScrFI       
SfaNI       StyI        TaqI        TseI        TspRI       
TthHB8I     
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