Restriction map for pF1KB3650
 (Circular) (Six-base) MAPSORT of: pF1KB3650.seq  Check: 3206  from: 1  to: 4547



pF1KB3650  4547 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AccI GT'mk_AC

Cuts at:    920    1585     920 
   Size:        665    3882
 
Acc65I G'GTAC_C

Cuts at:    680    1090    1563     680 
   Size:        410     473    3664

  Fragments arranged by size: 

                3664     473     410
 
AclI AA'CG_TT

Cuts at:   4387    4387 
   Size:       4547
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2372    2804    3551    3819    2372 
   Size:        432     747     268    3100

  Fragments arranged by size: 

                3100     747     432     268
 
AflIII A'CryG_T

Cuts at:   1899    3003    3980    1899 
   Size:       1104     977    2466

  Fragments arranged by size: 

                2466    1104     977
 
AgeI A'CCGG_T

Cuts at:   2935    2935 
   Size:       4547
 
AhdI GACnn_n'nnGTC

Cuts at:    171     171 
   Size:       4547
 
AleI CACnn'nnGTG

Cuts at:   1265    1265 
   Size:       4547
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    940     972     940 
   Size:         32    4515
 
AlwNI CAG_nnn'CTG

Cuts at:   1531    1852    3419    1531 
   Size:        321    1567    2659

  Fragments arranged by size: 

                2659    1567     321
 
ApaI G_GGCC'C

Cuts at:    394     721     394 
   Size:        327    4220
 
ApaLI G'TGCA_C

Cuts at:   3317    3317 
   Size:       4547
 
ApoI r'AATT_y

Cuts at:   1551    3900    4156    1551 
   Size:       2349     256    1942

  Fragments arranged by size: 

                2349    1942     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4547
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4547
 
AvaI C'yCGr_G

Cuts at:   1567    1567 
   Size:       4547
 
BamHI G'GATC_C

Cuts at:   1572    1572 
   Size:       4547
 
BanI G'GyrC_C

Cuts at:    111     490     680    1090    1185    1563    1977    2225    2260 
   Size:        379     190     410      95     378     414     248      35

Cuts at:   2260     111 
   Size:       2398

  Fragments arranged by size: 

                2398     414     410     379     378     248     190      95
                  35
 
BanII G_rGCy'C

Cuts at:    236     394     721    1561    2591     236 
   Size:        158     327     840    1030    2192

  Fragments arranged by size: 

                2192    1030     840     327     158
 
BbeI G_GCGC'C

Cuts at:    494    1981    2229     494 
   Size:       1487     248    2812

  Fragments arranged by size: 

                2812    1487     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    766    1407     766 
   Size:        641    3906
 
BbvCI CC'TCA_GC

Cuts at:    548     548 
   Size:       4547
 
BciVI GTATCCnnnnn_n'

Cuts at:   2445    3206    4547    2445 
   Size:        761    1341    2445

  Fragments arranged by size: 

                2445    1341     761
 
BclI T'GATC_A

Cuts at:   2067    2067 
   Size:       4547
 
BglI GCCn_nnn'nGGC

Cuts at:    145     145 
   Size:       4547
 
BglII A'GATC_T

Cuts at:   2062    4099    2062 
   Size:       2037    2510
 
BlpI GC'TnA_GC

Cuts at:   1659    1659 
   Size:       4547
 
Bme1580I G_kGCm'C

Cuts at:    394     721    2172    2265    3321     394 
   Size:        327    1451      93    1056    1620

  Fragments arranged by size: 

                1620    1451    1056     327      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    414     954     965    1098    1759    1840    2175    4319     414 
   Size:        540      11     133     661      81     335    2144     642

  Fragments arranged by size: 

                2144     661     642     540     335     133      81      11
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    255    2914     255 
   Size:       2659    1888
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    397    1308    1448     397 
   Size:        911     140    3496

  Fragments arranged by size: 

                3496     911     140
 
Bpu10I CC'TnA_GC

Cuts at:    437     548     437 
   Size:        111    4436
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    501     592    1723    2602    3094    3392     501 
   Size:         91    1131     879     492     298    1656

  Fragments arranged by size: 

                1656    1131     879     492     298      91
 
BsaI GGTCTCn'nnnn_

Cuts at:    114     520     537    4251     114 
   Size:        406      17    3714     410

  Fragments arranged by size: 

                3714     410     406      17
 
BsaAI yAC'GTr

Cuts at:   1307    1900    1307 
   Size:        593    3954
 
BsaBI GATnn'nnATC

Cuts at:      6    2061    4098       6 
   Size:       2055    2037     455

  Fragments arranged by size: 

                2055    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    491     593    1978    2226    2928    4424     491 
   Size:        102    1385     248     702    1496     614

  Fragments arranged by size: 

                1496    1385     702     614     248     102
 
BsaWI w'CCGG_w

Cuts at:   1736    2257    2935    3209    3356    3767    1736 
   Size:        521     678     274     147     411    2516

  Fragments arranged by size: 

                2516     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    313     343     556     586     940     970    1267    1297    1480 
   Size:         30     213      30     354      30     297      30     183

Cuts at:   1480    1510     313 
   Size:         30    3350

  Fragments arranged by size: 

                3350     354     297     213     183      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    367     577     580     994    1345     367 
   Size:        210       3     414     351    3569

  Fragments arranged by size: 

                3569     414     351     210       3
 
BseYI C'CCAG_C

Cuts at:    555    1043    1515    1524    1972    2730    3307    3803     555 
   Size:        488     472       9     448     758     577     496    1299

  Fragments arranged by size: 

                1299     758     577     496     488     472     448       9
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    174     243     594     174 
   Size:         69     351    4127

  Fragments arranged by size: 

                4127     351      69
 
BsiEI CG_ry'CG

Cuts at:    104    2089    2135    3343     104 
   Size:       1985      46    1208    1308

  Fragments arranged by size: 

                1985    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    236    1561    2339    2529    3321     236 
   Size:       1325     778     190     792    1462

  Fragments arranged by size: 

                1462    1325     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    236     394     721    1561    2172    2265    2339    2529    2591 
   Size:        158     327     840     611      93      74     190      62

Cuts at:   2591    3321     236 
   Size:        730    1462

  Fragments arranged by size: 

                1462     840     730     611     327     190     158      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   3767    3767 
   Size:       4547
 
BspHI T'CATG_A

Cuts at:   3723    3723 
   Size:       4547
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1597    1811    2113    2494    1597 
   Size:        214     302     381    3650

  Fragments arranged by size: 

                3650     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     915    2839    2893    4377    4542      50 
   Size:        865    1924      54    1484     165      55

  Fragments arranged by size: 

                1924    1484     865     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2459    2459 
   Size:       4547
 
BsrFI r'CCGG_y

Cuts at:   1289    2545    2726    2935    1289 
   Size:       1256     181     209    2901

  Fragments arranged by size: 

                2901    1256     209     181
 
BssSI C'ACGA_G

Cuts at:   2818    3176    2818 
   Size:        358    4189
 
BstAPI GCAn_nnn'nTGC

Cuts at:    154    1131    1924     154 
   Size:        977     793    2777

  Fragments arranged by size: 

                2777     977     793
 
BstBI TT'CG_AA

Cuts at:   2908    2908 
   Size:       4547
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1267    1267 
   Size:       4547
 
BstYI r'GATC_y

Cuts at:      1    1572    2039    2062    2397    2643    3655    3741    3753 
   Size:       1571     467      23     335     246    1012      86      12

Cuts at:   3753    4099       1 
   Size:        346     449

  Fragments arranged by size: 

                1571    1012     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    461     482     503     461 
   Size:         21      21    4505

  Fragments arranged by size: 

                4505      21      21
 
BtgI C'CryG_G

Cuts at:    107    1154    1266     107 
   Size:       1047     112    3388

  Fragments arranged by size: 

                3388    1047     112
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1210    1485    2497    2678    2841    1210 
   Size:        275    1012     181     163    2916

  Fragments arranged by size: 

                2916    1012     275     181     163
 
BtsI GCAGTG_nn'

Cuts at:    582     674    1790     582 
   Size:         92    1116    3339

  Fragments arranged by size: 

                3339    1116      92
 
ClaI AT'CG_AT

Cuts at:    725     725 
   Size:       4547
 
DraI TTT'AAA

Cuts at:   1546    1546 
   Size:       4547
 
DraIII CAC_nnn'GTG

Cuts at:   1131    1131 
   Size:       4547
 
DrdI GACnn_nn'nnGTC

Cuts at:   2253    3111    2253 
   Size:        858    3689
 
EaeI y'GGCC_r

Cuts at:    906    1161    1202    1269    1291    1533    2132    2306    2697 
   Size:        255      41      67      22     242     599     174     391

Cuts at:   2697    2724    4069     906 
   Size:         27    1345    1384

  Fragments arranged by size: 

                1384    1345     599     391     255     242     174      67
                  41      27      22
 
EagI C'GGCC_G

Cuts at:   2132    2132 
   Size:       4547
 
EarI CTCTTCn'nnn_

Cuts at:    461     775    1072    2570    2780     461 
   Size:        314     297    1498     210    2228

  Fragments arranged by size: 

                2228    1498     314     297     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2755    3065    3211    4357    2755 
   Size:        310     146    1146    2945

  Fragments arranged by size: 

                2945    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    234    1559     234 
   Size:       1325    3222
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    397    1308    1448    2372    2804    3551    3819     397 
   Size:        911     140     924     432     747     268    1125

  Fragments arranged by size: 

                1125     924     911     747     432     268     140
 
EcoNI CCTnn'n_nnAGG

Cuts at:    772     772 
   Size:       4547
 
EcoO109I rG'GnC_Cy

Cuts at:    390     391     471     717     718    1686     390 
   Size:          1      80     246       1     968    3251

  Fragments arranged by size: 

                3251     968     246      80       1       1
 
EcoRI G'AATT_C

Cuts at:   1551    1551 
   Size:       4547
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2566    2566 
   Size:       4547
 
FspI TGC'GCA

Cuts at:   2328    2328 
   Size:       4547
 
HaeII r_GCGC'y

Cuts at:    494     795    1981    2229    3251    4230     494 
   Size:        301    1186     248    1022     979     811

  Fragments arranged by size: 

                1186    1022     979     811     301     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    113     145     157     189     519     551    2389    2419    2421 
   Size:         32      12      32     330      32    1838      30       2

Cuts at:   2421    2451     113 
   Size:         30    2209

  Fragments arranged by size: 

                2209    1838     330      32      32      32      30      30
                  12       2
 
HincII GTy'rAC

Cuts at:   1586    1586 
   Size:       4547
 
KasI G'GCGC_C

Cuts at:    490    1977    2225     490 
   Size:       1487     248    2812

  Fragments arranged by size: 

                2812    1487     248
 
KpnI G_GTAC'C

Cuts at:    684    1094    1567     684 
   Size:        410     473    3664

  Fragments arranged by size: 

                3664     473     410
 
MluI A'CGCG_T

Cuts at:   3980    3980 
   Size:       4547
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3218    3402    3218 
   Size:        184    4363
 
MscI TGG'CCA

Cuts at:   1204    1535    2308    1204 
   Size:        331     773    3443

  Fragments arranged by size: 

                3443     773     331
 
MslI CAynn'nnrTG

Cuts at:   1265    2536    2663    1265 
   Size:       1271     127    3149

  Fragments arranged by size: 

                3149    1271     127
 
MspA1I CmG'CkG

Cuts at:    188    1035    1043    1658    1972    2332    3345    3590     188 
   Size:        847       8     615     314     360    1013     245    1145

  Fragments arranged by size: 

                1145    1013     847     615     360     314     245       8
            
 
NaeI GCC'GGC

Cuts at:   1291    2728    1291 
   Size:       1437    3110
 
NarI GG'CG_CC

Cuts at:    491    1978    2226     491 
   Size:       1487     248    2812

  Fragments arranged by size: 

                2812    1487     248
 
NcoI C'CATG_G

Cuts at:    107    1154     107 
   Size:       1047    3500
 
NgoMIV G'CCGG_C

Cuts at:   1289    2726    1289 
   Size:       1437    3110
 
NspI r_CATG'y

Cuts at:   1600    2100    3007    1600 
   Size:        500     907    3140

  Fragments arranged by size: 

                3140     907     500
 
PciI A'CATG_T

Cuts at:   3003    3003 
   Size:       4547
 
PflMI CCAn_nnn'nTGG

Cuts at:   1531    1531 
   Size:       4547
 
PfoI T'CCnGG_A

Cuts at:    809     857     809 
   Size:         48    4499
 
PmeI GTTT'AAAC

Cuts at:   1546    1546 
   Size:       4547
 
PmlI CAC'GTG

Cuts at:   1307    1307 
   Size:       4547
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    940     972    3686    3718     940 
   Size:         32    2714      32    1769

  Fragments arranged by size: 

                2714    1769      32      32
 
PspOMI G'GGCC_C

Cuts at:    390     717     390 
   Size:        327    4220
 
PstI C_TGCA'G

Cuts at:   1594    2279    1594 
   Size:        685    3862
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4547
 
PvuII CAG'CTG

Cuts at:   1043    1972    2332    1043 
   Size:        929     360    3258

  Fragments arranged by size: 

                3258     929     360
 
RsrII CG'GwC_CG

Cuts at:   2742    2742 
   Size:       4547
 
SacI G_AGCT'C

Cuts at:    236    1561     236 
   Size:       1325    3222
 
SalI G'TCGA_C

Cuts at:   1584    1584 
   Size:       4547
 
SapI GCTCTTCn'nnn_

Cuts at:   2570    2780    2570 
   Size:        210    4337
 
SbfI CC_TGCA'GG

Cuts at:   1594    1594 
   Size:       4547
 
ScaI AGT'ACT

Cuts at:    643     787     943    1438    1510    4064     643 
   Size:        144     156     495      72    2554    1126

  Fragments arranged by size: 

                2554    1126     495     156     144      72
 
SfcI C'TryA_G

Cuts at:     33     900    1365    1590    2275    3268    3459      33 
   Size:        867     465     225     685     993     191    1121

  Fragments arranged by size: 

                1121     993     867     685     465     225     191
 
SfoI GGC'GCC

Cuts at:    492    1979    2227     492 
   Size:       1487     248    2812

  Fragments arranged by size: 

                2812    1487     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4547
 
SmaI CCC'GGG

Cuts at:   1569    1569 
   Size:       4547
 
SmlI C'TyrA_G

Cuts at:    516     607    1702    2617    3109    3371     516 
   Size:         91    1095     915     492     262    1692

  Fragments arranged by size: 

                1692    1095     915     492     262      91
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4547
 
SphI G_CATG'C

Cuts at:   1600    2100    1600 
   Size:        500    4047
 
StuI AGG'CCT

Cuts at:    522     675     522 
   Size:        153    4394
 
StyI C'CwwG_G

Cuts at:    107     878    1154    1199    1681    2035     107 
   Size:        771     276      45     482     354    2619

  Fragments arranged by size: 

                2619     771     482     354     276      45
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2130    2263    2722    2931    2130 
   Size:        133     459     209    3746

  Fragments arranged by size: 

                3746     459     209     133
 
TatI w'GTAC_w

Cuts at:    329     641     785     941    1436    1508    4062     329 
   Size:        312     144     156     495      72    2554     814

  Fragments arranged by size: 

                2554     814     495     312     156     144      72
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    202     234     556     588    4064    4096     202 
   Size:         32     322      32    3476      32     653

  Fragments arranged by size: 

                3476     653     322      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2344    2344 
   Size:       4547
 
XbaI T'CTAG_A

Cuts at:   1578    1578 
   Size:       4547
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    901    2042     901 
   Size:       1141    3406
 
XmaI C'CCGG_G

Cuts at:   1567    1567 
   Size:       4547
 
XmnI GAAnn'nnTTC

Cuts at:    586     586 
   Size:       4547
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AleI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AlfI     AscI    AvrII     BaeI
     BcgI    BfrBI    BmgBI     BmtI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII  BstZ17I    CspCI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PpuMI    PshAI     PsiI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI
     ZraI
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